Electrocardiographic reference values and configuration of electrocardiogram waves recorded in Black Bengal goats of different age groups

Aim: A study on age-related electrocardiographic (ECG) changes was conducted on 20 apparently healthy Black Bengal goats with no history of cardiac disorders during 2015-2016. Materials and Methods: The goats selected for the study belonged to four different age groups; Group 1: Goats up to 6 months of age, Group 2: Above 6 months and below 1 year of age, Group 3: Above 1 year and below 2 years of age, and Group 4: Above 2 years of age. The ECG was recorded with the animals in standing position using a 12-lead standard ECG recorder (Model-Cardiart-108 MK VII, manufactured by BPL, India). The paper speed was set to 25 mm/s with the sensitivity of the machine was adjusted at 1 (1 cm=mV). Results: The ECG parameters were compared within different age groups, and the data were analyzed statistically using SPSS 16.0 taking a significant level of 95% (p<0.05) in all cases. The lead-I ECG revealed a significant difference in amplitude of QRS complex, PR interval, QT interval, RR interval, PQ segment, ST segment, TP segment, and heart rate among some age groups. In bipolar limb lead-II, the amplitude of T-wave, RR interval, ST segment, TP segment, and heart rate was a significant difference among some age groups. Lead-III presented significant difference among age groups in different parameters such as QRS complex duration, T-wave duration, RR interval, ST segment, TP segment, and heart rate. Conclusion: The study concluded that there is a significant variation in the ECG parameters both in terms of values and configuration of ECG waves when age is taken into consideration. The results of the study might be used as a reference value for field veterinarians

Alternative oxidase causes cell type- and tissue-specific responses in mutator mice

Energetic insufficiency, excess production of reactive oxygen species (ROS), and aberrant signaling partially account for the diverse pathology of mitochondrial diseases. Whether interventions affecting ROS, a regulator of stem cell pools, could modify somatic stem cell homeostasis remains unknown. Previous data from mitochondrial DNA mutator mice showed that increased ROS leads to oxidative damage in erythroid progenitors, causing lifespan-limiting anemia. Also unclear is how ROS-targeted interventions affect terminally differentiated tissues. Here, we set out to test in mitochondrial DNA mutator mice how ubiquitous expression of the Ciona intestinalis alternative oxidase (AOX), which attenuates ROS production, affects murine stem cell pools. We found that AOX does not affect neural stem cells but delays the progression of mutator-driven anemia. Furthermore, when combined with the mutator, AOX potentiates mitochondrial stress and inflammatory responses in skeletal muscle. These differential cell type-specific findings demonstrate that AOX expression is not a global panacea for curing mitochondrial dysfunction. ROS attenuation must be carefully studied regarding specific underlying defects before AOX can be safely used in therapy.Peer reviewe

Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions

AbstractMitochondrial dysfunction elicits stress responses that safeguard cellular homeostasis against metabolic insults. Mitochondrial integrated stress response (ISRmt) is a major response to mitochondrial (mt)DNA expression stress (mtDNA maintenance, translation defects), but the knowledge of dynamics or interdependence of components is lacking. We report that in mitochondrial myopathy, ISRmt progresses in temporal stages and development from early to chronic and is regulated by autocrine and endocrine effects of FGF21, a metabolic hormone with pleiotropic effects. Initial disease signs induce transcriptional ISRmt (ATF5, mitochondrial one-carbon cycle, FGF21, and GDF15). The local progression to 2nd metabolic ISRmt stage (ATF3, ATF4, glucose uptake, serine biosynthesis, and transsulfuration) is FGF21 dependent. Mitochondrial unfolded protein response marks the 3rd ISRmt stage of failing tissue. Systemically, FGF21 drives weight loss and glucose preference, and modifies metabolism and respiratory chain deficiency in a specific hippocampal brain region. Our evidence indicates that FGF21 is a local and systemic messenger of mtDNA stress in mice and humans with mitochondrial disease.</div

A study on aetiopathogenesis and management of rectal prolapse in tertiary care centre in Odisha, India

Introduction: Rectal prolapse refers to a circumferential, full-thickness protrusion of the rectum through the anus and also has been called complete prolapse, or procidentia. Internal prolapse occurs when the rectal wall intussuscepts but does not protrude, and is more accurately described as internal intussusception. In adults, rectal prolapse is far more common among women. The diagnosis is obvious on inspection, except in the case of concealed rectal prolapse. At present, the fixation of the rectum to the sacral hollow is considered to be the most rational operation in the surgical management of complete rectal prolapse. The present study is undertaken at S.C.B.Medical college hospital, Cuttack to evaluate the aetio-pathological factors associated with rectal prolapse and the different surgical methods available to repair them. Methods: A hospital-based Cross-sectional study was done among the patients Presenting .with Rectal Prolapse either attending Surgery Out-Patient Department or admitted to the Department through Emergency Department and giving consent for the study. Results: In the study 91.2% were complete and&nbsp; 8.8% were partial prolapse. The maximum age group showing thus prolapse was from 31-50 years age group, with males having more incidence of prolapse than females. Among the females, multi-porous were having increased incidence of prolapse.&nbsp

Evaluation of surgical abdomen in pediatric subjects admitted for acute abdomen at our tertiary care centre

Acute abdominal pain is one of the most common problems in children admitted to the pediatric emergency department (ED), and often presents a diagnostic dilemma for primary clinicians. Acute abdominal pain in patients presenting to the ED is often diagnosed as a disorder that does not require surgical intervention, such as acute gastroenteritis, functional digestive disorders or constipation. Objectives of our study: to evaluate various etilogies of acute abdomen in children presenting to paediatric emergency department and to estimate the prevalence of surgical abdomen in children presenting to pediatric emergency department. Methodology: Demographic information of the patients was noted down from the hospital case records, which include age, gender. Presenting complaints, clinical signs, etiologies of acute abdomen, laboratory investigations results TLC, DC, CRP, USG findings, CT abdomen findings, time and date of admission, daily progress, time and date of discharge were noted. In traumatic group apart of these observations additional lab investigations like amylase, lipase, ALT, AST, total protein and A:G ratio were noted. Results: We had a total of 132 subjects in non-traumatic group out of which 73% had acute appendicitis, 14% had incarcerated hernia, 7% had instussception, 5% had perforation and 0.75% had torsion.&nbsp

Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions

Mitochondrial dysfunction elicits stress responses that safeguard cellular homeostasis against metabolic insults. Mitochondrial integrated stress response (ISRmt) is a major response to mitochondrial (mt)DNA expression stress (mtDNA maintenance, translation defects), but the knowledge of dynamics or interdependence of components is lacking. We report that in mitochondrial myopathy, ISRmt progresses in temporal stages and development from early to chronic and is regulated by autocrine and endocrine effects of FGF21, a metabolic hormone with pleiotropic effects. Initial disease signs induce transcriptional ISRmt (ATF5, mitochondria) one-carbon cycle, FGF21, and GDF15). The local progression to 2nd metabolic ISRmt stage (ATF3, ATF4, glucose uptake, serine biosynthesis, and transsulfuration) is FGF21 dependent. Mitochondria! unfolded protein response marks the 3rd ISRmt stage of failing tissue. Systemically, FGF21 drives weight loss and glucose preference, and modifies metabolism and respiratory chain deficiency in a specific hippocampal brain region. Our evidence indicates that FGF21 is a local and systemic messenger of mtDNA stress in mice and humans with mitochondrial disease.Peer reviewe