Identifikasi Dan Analisis Akrilamida Dalam Kopi Serbuk (Tubruk) Dan Kopi Instan Dengan Metode Kromatografi Cair Kinerja Tinggi

Acrylamide is a substance that can cause cancer on human and is neurotoxic. Acrylamide is formed due to high temperature heating of foods that contains carbohydrates and amino acids. Carbohydrates and amino acids are the major compounds that contained in coffee beans. This study aims to determine the levels of acrylamide in ground coffee and instant coffee that have different process of manufacture. Method of analyze of acrylamide were perfomed by HPLC (High Performance of Liquid Chromatography) method using mobile phase that consists of phosphoric acid : acetonitrile : aquabides (1:5:94 v/v/v), the stationary phase was Sunfire C18 column (150 x 4.6 mm, 5μm), and the flow rate was 0-15 mL/minute and the detection using UV 202 nm. The result of the study was validation of method that provide the linearity 0.999 (range 2-20 μg/mL), LOD of 0.94 μg/mL and LOQ of 2.86 μg/mL, the precision with RSD of 0.47%, and accuracy for ground coffee of 91-94% and instant coffee of 99-102%. The study found acrylamide levels in ground coffee and instant coffee were 7.03 ±0.01 μg/g dan 5.71 ± 0.03 μg/g respectively. These levels were considered safe for up to 16 g for consume of coffee

Similarity Check: PERBANDINGAN AKTIVITAS ANTIINFLAMASI ANTARA MINYAK ATSIRI DAN FRAKSI ETANOL RIMPANG DRINGO (Acorus calamus, L.) PADA TIKUS PUTIH JANTAN

Telah dilakukan penelitian aktivitas antiinflamasi dari rimpang dringo (Acorus calamus, L). Aktivitas antiinflaması dikaji dengan membandingkan pengaruh pemberian minyak atsiri & fraksi etanol terhadap penurunan volume udem buatan yang ditimbulkan oleh penyuntikan 0,10 ml karagenin 1% subplantar dalam natrium klorida fisiologis. Penelitian ini menggunakan 20 ekor tikus putih jantan galur wistar dengan berat badan berkisar antara 150-200 gram. Tikus dibagi menjadi 4 kelompok, masing-masing 5 ekor tikus sebelum perlakuan, tikus dipuasakan 18 jam. Kelompok I hanya diradangkan dengan karagenin 1% secara subplantar. Untuk kelompok lainnya, sesaat sebelum diberi kargenin 1% subplantar, masing-masing diberi perlakuan, yaitu untuk kelompok Il diberi minyak atsiri Dringo. kelompok III diberi fraksi etanol Dringo, Kelompok IV diberi Natrium Diklofenak, Volume udem diukur pada jam ketiga, setelah pemberian karagenin dengan alat plestismograf Hasil penelitian menunjukkan untuk kelompok II diberi minyak atsiri dringo 100 mg kg BB mempunyai daya antinflamasi 52,81 ± 29,13, dan kelompok III diberi fraksi etanol dringo 100 mg/kgBB mempunyai daya antiinflamasi 28,56 ± 33,57%. Hasil yang diperoleh menunjukkan tidak ada perbedaan yang bermakna daya antiinflamasi antara fraksi etanol dengan minyak atsiri rimpang dringo terhadap natrium diklofenak

Pengaruh Penambahan Bit (Beta Vulgaris L.) Sebagai Pewarna Alami Dan Bahan Pengisi Terhadap Karakteristik Fisikokimia Dan Sensori Sosis Nabati

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan berbagai konsentrasi pasta bit terhadap karakteristik fisik (tekstur danwarna), karakteristik kimia (analisis proksimat dan aktivitas antioksidan), dan karakteristik sensori sosis nabati yang terbuat dari jamur tiram putih.Analisis proksimat meliputi kadar abu, kadar lemak, kadar air, kadar karbohidrat, dan kadar protein. Pada seluruh analisis digunakan RancanganAcak Lengkap (RAL) dengan satu faktor yaitu variasi konsentrasi pasta bit pada sosis nabati. Konsentrasi pasta bit yang ditambahkan 0%, 5%,10% and 15. Karakteristik sensori meliputi parameter tekstur, warna, aroma, rasa dan overall. Karakteristik kimia yang diamati meliputi analisisproksimat dan aktivitas antioksidan. Karakteristik fisik yang diuji meliputi tekstur dan warna. Hasil penelitian menunjukkan bahwa pada analisissensoris (Uji Perbandingan Jamak), panelis dapat membedakan sosis yang ditambah pasta bit pada konsentrasi 0%, 5%, 10%, dan 15%.Penambahan pasta bit sebanyak 5%, 10% dan 15% dapat meningkatkan warna produk tanpa menyebabkan Perubahan rasa, bau, dan tekstur, dansecara hedonik panelis memilih penambahan pasta bit 10% sebagai perlakuan terbaik. Hal ini juga berdasarkan pertimbangan dari segi efisiensibahan. Kadar abu, kadar lemak dan kadar protein memiliki nilai yang cenderung konstan seiring dengan meningkatnya konsentrasi pasta bit. Kadarair, aktivitas antioksidan dan warna memiliki nilai yang semakin meningkat seiring meningkatnya konsentrasi pasta bit yang digunakan,konsentrasi 15% memiliki nilai tertinggi dan pada konsentrasi 0% memiliki nilai terendah. Pada analisis tekstur dan kadar karbohidrat diketahuinilai semakin menurun seiring meningkatnya konsentrasi pasta bit yang digunakan, diperoleh hasil bahwa konsentrasi 0% memiliki nilai tertinggidan konsentrasi 15% memiliki nilai terendah

Standardization of Leaf Extract of Red Betel(Piper Crocatum) Leaves Using Ethanol

Background: Red betel vine (Piper crocatum Ruiz & Pav) is known empirically to have properties to cure various kinds of diseases. It contains flavonoids, alkaloids, polifenolat compounds, tannins and essential oil compounds. Standardization in the pharmacy is required to ensure the level of quality, fulfill the standard requirement of chemical, biological, and pharmaceutical, including the guarantee of stability as pharmaceutical products generally.Objective: To investigate the standard specific and non-specific parameters set by Food and Drug Supervisory Agency (FDSA) of ethanol leaf extract of red betel leaves.Methods: The study began with sample preparation, then extracted by maceration method to get the active compounds in the lumpy extract. Extract was analyzed with standard specific and non-specific parameters set by FDSA. Results were analyzed with descriptive analysis method.Results: The features of leaf extract of red betel vine leaves using are organolepticly viscous, dark green,and has a distinctive odor with a bitter spicy taste. The yield, water content, ash content, and specific gravity of extract were 14.8%, 0.353%, 0.16%, 0.729 respectively. Total mold contamination of extract satisfied the standard criteria that was below the limit of a maximum of 10 colonies/gram. Metal contamination of lead (Pb) was 1. 404 mg/kg and Cadmium (Cd) was 0. 223 mg/kg. Chromatographic profile of the Gas Chromatography-Mass Spectrometry (GC-MS) red betel vine leaves ethanol extract contains compounds caryophyllene, germacrene-D and some other compounds with low similiaritas index, and has a marker compound which is suspected possibly trimethoxyallyl benzen.Conclusion : The ethanol extract of red betel vine (Piper crocatum Ruiz & Pav) leaves is an extract of the organolepticly viscous, dark green color, distinctive smell, bitter taste, and spicy. The ethanol extract of red betel vine leaves satisfies the standard level set by FDSA and has the marker compound which is expected likely Trimethoxyallyl benzen

Pengembangan Dan Validasi Metode Analisis Rifampicin Isoniazid-pirazinamid Dalam Fixed Dose Combination Dengan Metode Kromatografi Lapis Tipis-densitometri

Rifampicin, isoniazid (INH) and pyrazinamide are anti tuberculosis drugs (ATD) available in fixed dose combination (FDC) form. The FDC is more practical in USAge so can improve tuberculosis patient obedience in consuming the drug. However, in some researches, there are still found subdose of FDC. Subdose of FDC that effected in less optimal TB medication and increase risk of ATD resistance. The high TB case in developing countries such as Indonesia require test of FDC drug dose evaluation. Objective of this research was to develop new analytical method, Thin Layer Chromatography (TLC)-densitometry having good validity so it may be easier, faster, cheaper and more practical analytical method alternative. Validation parameters consist of linearity, precision, accuracy, Limit Of Detection (LOD), and Limit Of Quantitation (LOQ). Parameter of method validation results was compared with requirement in Association of Official Analytical Chemist (AOAC) and United States pharmacopeia (USP) for determine active ingredient in sample. FDC sample containing rifampicin, INH and pyrazinamide can be separated with n-hexane: 2-propanol: acetone: ammonia: formic acid with proportion of 3:3.6:3:0.3:0.1 (v/vv/v) as mobile phase and Rf value for rifampicin, INH, and pyrazinamide were 0.85, 0.6, and 0.7, respectively. The results indicated that TLC-densitometry can be developed and all validation parameters complied with AOAC requirements. The correlation coefficient (r) of rifampicin 0.999, INH 0.999 and pyrazinamide 0.999; recovery of rifampicin, INH and pyrazinamide were 101.00 %, 94.36 % and 95.69 %, respectively. In addition, precision, % RSD for rifampicin, INH and pyrazinamide were 0.55 %, 0.96 %, and 0.98 % respectively; LOD for rifampicin, INH and pyrazinamide were 10.91 ppm, 10.38 ppm and 42.14 ppm, respectively; LOQ for rifampicin, INH and pyrazinamide were 33.07 ppm, 31.45 ppm and 127.7 ppm, respectively. Concentration of rifampicin, INH and pyrazinamide in a tablet were 157.37 mg, 75.26 mg and 400.79 mg that comply with USP standard

Types and Abundance of Phytoplankton of the Boko-Boko Peat Swamp in the Langgam Village, Langgam District, Pelalawan Regency, Riau Province

The area around the Boko-Boko peat swamp has been used for palm plantation. Remains of fertilizer and pestiside used in the plantation may enter the water and decrease the water quality of the swamp and thus reduce the water quality in general. To understand the type and abundance of phytoplankton in the Boko-Boko peat swamp's waters, a research was conducted in March 2016. There were three sampling stations namely Station 1, Station 2, and Station 3. Samplings were conducted 3 times, once/week. Results shown that there are 18 species of phytoplankton present in the peat swamp. They were classified into four classes, namely: Bacillariophyceae (6 species), Chloropyhceae (1 species), Cyanophyceae (5 species) and Xanthophycea (3 Species). The average of phytoplankton abundance was around 10,948 – 18,186 cells/L, species diversity index (H') was 2.082 -2.424, index equatibility (E) was 0.81 – 0.86 and dominance index (C) was 0.218 – 0.288. While the water quality parameters were as follows: temperature 30.1 – 30.4 0C, transparency 48.6 - 60 cm, depth 148.3 – 180 cm, pH 5, CO2 : 7.32 – 9.99 mg/L, DO : 2.54 – 3.26 mg/L, nitrate : 0.4444 – 0.6436 mg/L and phosphate : 0.7779 – 0.123 mg/L. Based on the abundance of pyhtoplankton, it can be concluded that the peat swamp Boko - Boko can be categorized as mesotropic

Studi Tentang Pengaruh Penggunaan Teknologi dan Sistem Informasi, Sistem Manajemen Mutu, dan Budaya Organisasi terhadap Kinerja Manajemen Perguruan Tinggi Swasta: Studi Kasus Universitas Bina Nusantara

Article discusses factors that influences college management performance, those are technology and information system, quality management system, and organization culture. The research is done by using Pearson correlation method, linear regression, and Customer Satisfaction Index (CSI) using cartesius diagram. Based on correlation test, there are only two out of three variables having significance correlation with performance management, that are quality management system and organization culture

Sistem Pendukung Keputusan untuk Pemberian Reward Kader Terbaik Organisasi Ikatan Mahasiswa Muhammadiyah Kota Bengulu Menggunakan Algoritma Simple Additive Weighting

Ikatan Mahasiswa Muhammadiyah disingkat IMM adalah gerakan Mahasiswa Islam yang beraqidah Islam bersumber al-Quran dan As-Sunah. Pendapatan keanggotaan Ikatan Mahasiswa Muhammadiyah yang berupa Anggota Biasa, Anggota Luar biasa dan Anggota Kehormatan memerlukan sistem pendukung keputusan untuk menunjang kondisi internal yang baik di level Pimpinan di Kota Bengkulu. Pengambilan keputusan dianggap sebagai sautu proses pemilihan suatu jalur dengan beberapa alternatif-alternatif yang ada, setiap pengambilan keputusan selalu menghasilkan satu pilihan. Seorang anggota organisasi yang menerima reward tentunya harus memenuhi beberapa kriteria-kriteria tertentu yang berhubungan dengan kriteria yang ditentukan oleh masing-masing organisasi. Algoritma SAW (Simple Additive Weighting) dikenal istilah metode penjumlahan terbobot adalah menggunakan proses normalisasi matriks keputusan yang dibandingkan dengan semua alternatif yang ada dalam mencari rating atau kinerja, metode penyelasaian masalah multi atribut ini paling sederhana paling banyak digunakan merupakan metode paling mudah diaplikasikan karena mempunyai algoritma yang tidak terlalu rumit. Penelitian ini bertujuan untuk memberikan kemudahan kepada organisasi IMM dalam menilai dan memberikan reward kepada anggotanya