Soluble Fraction of Trichosanthes diocia Peroxidase in Decolorization of Reactive Orange 15

Salt fractionated proteins from Trichosanthes diocia was used to study decolorization of Reactive Orange 15 under multifarious experimental conditions like pH, temperature, time interval, enzyme concentration and in the presence of redox mediator 1-hydroxybenzotriazole (HOBT)/ vanillin. T. diocia peroxidase showed extensive decolorization of Reactive Orange15 in the presence of 1-hydroxybenzotriazole (HOBT) / vanillin. The dye was decolorized effectively with HOBT and so further studies were performed in the presence of HOBT.  At an enzyme concentration of 0.45 EUmL-1 the peroxidase was able to remove the reactive dye up to a maximum of 94.6% with 1.0 mM 1-hydroxybenzotriazole. Maximum decolorization was achieved at a temperature of 40°C, pH 5.0 and with an incubation period of 90 min. Thus, the data indicates that T. diocia peroxidase could be a potential source for developing an inexpensive and efficient method for the treatment of recalcitrant Reactive Orange15 dyes that are potentially toxic. ---Department of Biochemistry (DST-FIST & UGC-SAP Supported), Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P. (India)---Please Cite This Article As: Farrukh Jamal, Tabish Qidwai and Prabhash K Pandey. 2010. Soluble Fraction of Trichosanthes diocia Peroxidase in Decolorization of Reactive Orange 15. J. Ecobiotechnol. 2(5):36-41.Â

A Comparative Study on Environmental Flows Assessment Methods in Lower Reach of Mahanadi River

Environmental flows assessment is a process which determines the allocated water for maintaining aquatic habitats and ecological processes in a environment. The river system attains zero flow in low flow period due to construction of hydropower generating structures, water retaining structure and withdrawal of water by water users, which possesses a tremendous threat to the environment, ecology & aquatic life. Therefore a need arises to regulate the reservoirs for releasing the adequate water in the river throughout the year as well as a flushing flow once in a year. Thus environmental flows assessment is done in Lower Mahanadi sub-basin and recommended to provide the EFRs on average 26 % of MAF with a range of Low flow 56% of mean low flow & High flow 21% of mean high flow to be ensured at any circumstances to avoid any degradation of river ecosystem. In the present study the assessment of the environmental flows on the basis of Tennant method, Tessman method, VMF method, Q90_Q50 method, Smakhtin method,  low flow index (7Q10) method and FDC (EMC shifting technique) method using Global Environmental Flow Calculator (GEFC) software. Keywords: EFR, GIS, Tennant, Tessman, VMF, Q90_Q50, 7Q10, FDC method

Optimizing University Mobility : An Internal Navigation and Crowd Management System

In the evolving landscape of educational technology, the article explores the critical frontier of indoor navigation systems, focusing on universities. Traditional approaches in higher education often fall short of meeting dynamic user expectations, necessitating revolutionary solutions. This research introduces an innovative internal navigation and crowd management system that seamlessly integrates augmented reality, natural language processing, machine learning, and image processing technologies. The Android platform serves as the foundation, harnessing augmented reality's transformative capabilities to provide real-time visual cues and personalized wayfinding experiences. The voice interaction module, backed by NLP and ML, creates an intelligent, context-aware assistant. The crowd management module, employing advanced image processing, delivers real-time crowd density insights. Personalized recommendations, powered by NLP and ML, offer tailored canteen suggestions based on user preferences. The agmented reality navigation module, using Mapbox, Unity Hub, AR Core, and Vuforia, enriches the user experience with dynamic visual cues. Results reveal the success of each module: the voice interaction module showcases continuous learning, user-centric feedback, contextual guidance excellence, robust security, and multimodal interaction flexibility. The crowd management module excels in video feed processing, image processing with OpenCV, and real-time availability information retrieval. The personalized recommendations module demonstrates high accuracy, equilibrium, and robust performance. The AR navigation module impresses with precision, enriched navigation, and tailored routes through machine learning. This cohesive system sets new benchmarks for user-centric technology in universities. Future work includes multi-university integration, intelligent spatial design, and real-time decision support, paving the way for more efficient, user-centered university experiences and contributing to the advancement of smart university environments. The research serves as a pivotal force in reshaping interactions within university spaces, envisioning a future where technology seamlessly enhances the essence of human interaction in educational environments

Learning Management System Built Using the MERN Stack

Web based applications play a main role in our day-to-day life and therefore, it is important to ensure the quality and reliability of web applications. With the sudden increase in use of web based applications for online and distant learning, it is important to address existing issues in current Learning Management Systems (LMS), so that users can benefit from a better, uninterrupted learning experience. This work mainly contributes to understanding how the MERN stack can be efficiently used in building a reliable and secure LMS that will provide its services free of charge so that students are provided with a free and uninterrupted learning experience. An LMS that is equipped with user handling functionality, managing courses and course materials, and maintaining a library, has been the focus of the study that resulted in producing this paper. The work also includes reasoning as to why the MERN stack was selected for developing the proposed system

Understanding future water challenges in a highly regulated Indian river basin — modelling the impact of climate change on the hydrology of the upper Narmada

The Narmada river basin is a highly regulated catchment in central India, supporting a population of over 16 million people. In such extensively modified hydrological systems, the influence of anthropogenic alterations is often underrepresented or excluded entirely by large-scale hydrological models. The Global Water Availability Assessment (GWAVA) model is applied to the Upper Narmada, with all major dams, water abstractions and irrigation command areas included, which allows for the development of a holistic methodology for the assessment of water resources in the basin. The model is driven with 17 Global Circulation Models (GCMs) from the Coupled Model Intercomparison Project Phase 5 (CMIP5) ensemble to assess the impact of climate change on water resources in the basin for the period 2031–2060. The study finds that the hydrological regime within the basin is likely to intensify over the next half-century as a result of future climate change, causing long-term increases in monsoon season flow across the Upper Narmada. Climate is expected to have little impact on dry season flows, in comparison to water demand intensification over the same period, which may lead to increased water stress in parts of the basin

Two successful pregnancies in a woman with chronic myeloid leukemia exposed to nilotinib during the first trimester of her second pregnancy: case study

The occurrence of chronic myeloid leukemia in pregnancy is rare and its management poses a clinical challenge for physicians treating these patients. We report a 30-year-old woman with chronic myeloid leukemia who became pregnant twice successfully. Philadelphia-positive CML in its chronic phase was diagnosed at 16 weeks of her first gestation. At that time, she received no treatment throughout her pregnancy. At 38 weeks of gestation, a normal infant was delivered by cesarean section. At six weeks postpartum, the patient underwent imatinib mesylate therapy but she could not tolerate the treatment. The treatment was then changed to nilotinib at 400 mg orally b.i.d. Two years later, she became pregnant again while she was on nilotinib 200 mg b.i.d. The unplanned pregnancy was identified during her 7.4 weeks of gestation. Because the patient elected to continue her pregnancy, nilotinib was stopped immediately, and no further treatment was given until delivery. Neither obstetrical complications nor structural malformations in neonates in both pregnancies were observed. Both babies' growth and development have been normal. Although this experience is limited to a single patient, the success of this patient demonstrates that the management of chronic myeloid leukemia in pregnant women may be individualized based on the relative risks and benefits of the patient and fetus

Data for: Salvage surgery in head and neck cancer: Does it improve outcomes?

Whether salvage surgery after chemoradiation in recurrent or residual head and neck cancer improves outcomes is an open question. The proponents of salvage surgery point to the multiple small, retrospective, single-center analyses performed across the globe which hint towards the efficacy of salvage surgery. The opponents on the other hand point that diseases with small volumes that are accessible without involving critical structures are usually selected for salvage surgery. This selection gives a biological advantage to patients undergoing salvage surgery over those treated with non-surgical modalities and therefore it is possible that the improved outcomes seen with salvage surgery are a result of selection bias. To answer this question we performed this analysis.This was a post hoc analysis of data from a phase 3 study comparing the efficacy of cisplatin - radiation versus Nimotuzumab-cisplatin-radiation. Patients in this study who had the recurrent or residual disease and who were eligible for salvage surgery were selected for this study. These were divided into 2 cohorts for comparison, willing for salvage surgery (n=91) and unwilling for salvage surgery(n=22). The primary endpoint was overall survival. This we thought was a better method of comparison than who underwent salvage surgery versus did not undergo salvage surgery. As some patients even who are willing for salvage surgery progress before the surgery. This biologically bad subgroup deteriorates the results of patients who did not undergo salvage surgery group. We feel this analysis is close to perfect as performing a randomized study in this setting seems unethical after observing our results. The results are in favor of salvage surgery