Simulating the detection of the global 21 cm signal with MIST for different models of the soil and beam directivity

The Mapper of the IGM Spin Temperature (MIST) is a new ground-based, single-antenna, radio experiment attempting to detect the global 21 cm signal from the Dark Ages and Cosmic Dawn. A significant challenge in this measurement is the frequency-dependence, or chromaticity, of the antenna beam directivity. MIST observes with the antenna above the soil and without a metal ground plane, and the beam directivity is sensitive to the electrical characteristics of the soil. In this paper, we use simulated observations with MIST to study how the detection of the global 21 cm signal from Cosmic Dawn is affected by the soil and the MIST beam directivity. We simulate observations using electromagnetic models of the directivity computed for single- and two-layer models of the soil. We test the recovery of the Cosmic Dawn signal with and without beam chromaticity correction applied to the simulated data. We find that our single-layer soil models enable a straightforward recovery of the signal even without chromaticity correction. Two-layer models increase the beam chromaticity and make the recovery more challenging. However, for the model in which the bottom soil layer has a lower electrical conductivity than the top layer, the signal can be recovered even without chromaticity correction. For the other two-layer models, chromaticity correction is necessary for the recovery of the signal and the accuracy requirements for the soil parameters vary between models. These results will be used as a guideline to select observation sites that are favorable for the detection of the Cosmic Dawn signal.Comment: Accepted for publication in the Astrophysical Journa

Inorganic polyphosphate accumulation suppresses the dormancy response and virulence in Mycobacterium tuberculosis

Stringent response pathways involving inorganic polyphosphate (PolyP) play an essential role in bacterial stress adaptation and virulence. The intracellular levels of PolyP are modulated by the activities of polyphosphate kinase-1 (PPK1), polyphosphate kinase-2 (PPK2), and exopolyphosphatases (PPXs). The genome of Mycobacterium tuberculosis encodes two functional PPXs, and simultaneous deletion of ppx1 and ppx2 results in a defect in biofilm formation. We demonstrate here that these PPXs cumulatively contribute to the ability of M. tuberculosis to survive in nutrient-limiting, low-oxygen growth conditions and also in macrophages. Characterization of single (Delta ppx2) and double knockout (dkppx) strains of M. tuberculosis indicated that PPX-mediated PolyP degradation is essential for establishing bacterial infection in guinea pigs. RNA-Seq-based transcriptional profiling revealed that relative to the parental strain, the expression levels of DosR regulon-regulated dormancy genes were significantly reduced in the dkppx mutant strain. In concordance, we also provide evidence that PolyP inhibits the autophosphorylation activities associated with DosT and DosS sensor kinases. The results in this study uncover that enzymes involved in PolyP homeostasis play a critical role in M. tuberculosis physiology and virulence and are attractive targets for developing more effective therapeutic interventions