How to Measure Group Selection in Real-world Populations

Multilevel selection and the evolution of cooperation are fundamental to the formation of higher-level organisation and the evolution of biocomplexity, but such notions are controversial and poorly understood in natural populations. The theoretic principles of group selection are well developed in idealised models where a population is neatly divided into multiple semi-isolated sub-populations. But since such models can be explained by individual selection given the localised frequency-dependent effects involved, some argue that the group selection concepts offered are, even in the idealised case, redundant and that in natural conditions where groups are not well-defined that a group selection framework is entirely inapplicable. This does not necessarily mean, however, that a natural population is not subject to some interesting localised frequency-dependent effects – but how could we formally quantify this under realistic conditions? Here we focus on the presence of a Simpson’s Paradox where, although the local proportion of cooperators decreases at all locations, the global proportion of cooperators increases. We illustrate this principle in a simple individual-based model of bacterial biofilm growth and discuss various complicating factors in moving from theory to practice of measuring group selection

The Efficacy of Group Selection is Increased by Coexistence Dynamics within Groups

Selection on the level of loosely associated groups has been suggested as a route towards the evolution of cooperation between individuals and the subsequent formation of higher-level biological entities. Such group selection explanations remain problematic, however, due to the narrow range of parameters under which they can overturn within-group selection that favours selfish behaviour. In principle, individual selection could act on such parameters so as to strengthen the force of between-group selection and hence increase cooperation and individual fitness, as illustrated in our previous work. However, such a process cannot operate in parameter regions where group selection effects are totally absent, since there would be no selective gradient to follow. One key parameter, which when increased often rapidly causes group selection effects to tend to zero, is initial group size, for when groups are formed randomly then even moderately sized groups lack significant variance in their composition. However, the consequent restriction of any group selection effect to small sized groups is derived from models that assume selfish types will competitively exclude their more cooperative counterparts at within-group equilibrium. In such cases, diversity in the migrant pool can tend to zero and accordingly variance in group composition cannot be generated. In contrast, we show that if within-group dynamics lead to a stable coexistence of selfish and cooperative types, then the range of group sizes showing some effect of group selection is much larger

Study of aerodynamic technology for single-cruise engine V/STOL fighter/attack aircraft

A conceptual design analysis is performed on a single engine V/STOL supersonic fighter/attack concept powered by a series flow tandem fan propulsion system. Forward and aft mounted fans have independent flow paths for V/STOL operation and series flow in high speed flight. Mission, combat and V/STOL performance is calculated. Detailed aerodynamic estimates are made and aerodynamic uncertainties associated with the configuration and estimation methods identified. A wind tunnel research program is developed to resolve principal uncertainties and establish a data base for the baseline configuration and parametric variations

OPAD 1991

The Optical Plume Anomaly Detection Program, an experimental study in the attempt to create a rocket engine health monitor based on detection and possible quantification of anomalous atomic and molecular species in the exhaust plume, has been in existence for several years. The instruments developed to monitor the exhaust plumes are presented. Two optical instruments are employed: the polychromator and the spectrometer. The polychromator is a 16 channel spectroradiometer having independent channels individually adjustable for center wavelength and pass bandwidth. Those values may be mechanically reset, with the aid of certain lab equipment. The spectrometer is a multichannel spectral analyzer having two 2048 element linear photodiode arrays at the exit plane of the dispersing instrument, which, like the 16 channel device, is a 1/2 meter grating spectrograph. These instruments are equipped with ultraviolet grade multifiber optical input cables, allowing the instruments to be placed in a benign environment. Telescopes mounted on the test stand observe the plume keeping the shock structure in view. The data acquisition and control system consist of four 80386-33 MHz computers: two at the test stand for instrument control and data preconditioning, and two in the test support building providing data archiving, display, and system control. Further descriptions of the instrumentation are provided

How to measure group selection in real-world populations

Multilevel selection and the evolution of cooperation are fundamental to the formation of higher-level organisation and the evolution of biocomplexity, but such notions are controversial and poorly understood in natural populations. The theoretic principles of group selection are well developed in idealised models where a population is neatly divided into multiple semi-isolated sub-populations. But since such models can be explained by individual selection given the localised frequency-dependent effects involved, some argue that the group selection concepts offered are, even in the idealised case, redundant and that in natural conditions where groups are not well-defined that a group selection framework is entirely inapplicable. This does not necessarily mean, however, that a natural population is not subject to some interesting localised frequency-dependent effects -- but how could we formally quantify this under realistic conditions? Here we focus on the presence of a Simpson's Paradox where, although the local proportion of cooperators decreases at all locations, the global proportion of cooperators increases. We illustrate this principle in a simple individual-based model of bacterial biofilm growth and discuss various complicating factors in moving from theory to practice of measuring group selection.Comment: pp. 672-679 in Proceedings of the Eleventh European Conference on the Synthesis and Simulation of Living Systems (Advances in Artificial Life, ECAL 2011). Edited by Tom Lenaerts, Mario Giacobini, Hugues Bersini, Paul Bourgine, Marco Dorigo and Ren\'e Doursat. MIT Press (2011). http://mitpress.mit.edu/catalog/item/default.asp?ttype=2&tid=12760. 8 pages, 5 figure

First Report of Columbia Root Knot Nematode (\u3ci\u3eMeloidogyne chitwoodi\u3c/i\u3e) in Potato in Texas

Columbia root-knot nematode, Meloidogyne chitwoodi Golden et al. (1) was identified from potatoes, Solanum tuberosum L., collected from Dallam County, Texas in October 2000. Seed potatoes are the most likely source for this introduction. This nematode is currently found infecting potatoes grown in California, Colorado, Idaho, New Mexico, Nevada, Oregon, Utah, and Washington. Some countries prohibit import of both seed and table stock potatoes originating in states known to harbor M. chitwoodi. Lesions on the potatoes had discrete brown coloration with white central spots in the outer 1 cm of the tuber flesh. Female nematode densities averaged 3 per square centimeter of a potato section beneath the lesions. Nematodes were morphologically identified as M. chitwoodi based on the perineal pattern of mature females and the tail shape of juveniles per Golden et al. (1)

Effects of Preservation Methods, Parasites, and Gut Contents of Black Flies (Diptera: Simuliidae) on Polymerase Chain Reaction Products

Molecular analysis of biological specimens usually requires extraction of high-molecular-weight DNA free of foreign DNA contaminants. DNA was extracted from black flies at different life stages that had been preserved by 4 methods: larvae and adults in ethanol, larvae in Carnoy’s solution, adults on card-points, and adults hand-swatted and sun-dried. Using specific primers for the mitochondrial ND4 gene, a 257-bp amplicon was obtained from specimens preserved by ethanol, card-point mounting, and sun-drying. Successful amplification often required DNA dilutions ≥ 1:20 (\u3c1–10 ng). DNA from specimens preserved in Carnoy’s solution (ethanol: acetic acid, 3:1) yielded degraded DNA, resulting in fewer successful amplifications. Parasitic nematodes and, to a lesser extent, gut contents resulted in extra products when amplified with randomly amplified polymorphic DNA (RAPD) primers. Sufficient DNA was extracted from the head of a larva for a successful polymerase chain reaction (PCR), eliminating the need to remove the contaminating gut and parasites