Optimisation of polymer optical fibre based interferometric sensors

A numerical model for studying the performance of polymer optical fibre-based interferometric sensors is presented. The strain sensitivity of Fabry-Perot and two-beam interferometric sensors is investigated by varying the physical and optical properties corresponding to frequently used wavelengths. The developed model was used to identify the regimes in which these devices offer enhanced performance over their silica counterparts when used for stress sensing. © (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only

Time-dependent variation of POF Bragg grating reflectivity and wavelength submerged in different liquids

In this work, we investigate the time-dependent variation of both the reflectivity and resonance wavelength of microstructured polymer optical fiber Bragg grating (mPOFBG) array sensors embedded in silicone rubber and polyurethane resin diaphragms in contact with water and aircraft fuel, respectively. The array sensors were inscribed using two different phase masks with pitches of 557.5 and 580 nm and the thermal annealing of the inscribed fiber was used to change the Bragg wavelengths. Both the reflection and the resonance wavelength shift were monitored over 90 days submerged in liquid and two studies were investigated. In the first study, in addition to the mPOFBGs coated with the diaphragm, also the rest of the fiber is totally protected between the sensors with the same material used for diaphragms. On the other hand, in the second study, the fiber between sensors is unprotected – in direct contact with liquid. PMMA and TOPAS fibers were used and this study suggests that TOPAS fiber should be a good option for long-term liquid monitoring applications

WT1 TCR gene transfer into haematopoietic stem cells: In vivo functional analysis of WT1-specific T cells

The Wilms tumour antigen is a promising target for T cell-based tumour immunotherapies. Vaccines against WT1 peptides tested in cancer patients showed immunological and molecular responses. However, the clinical responses observed were partial and it is currently not known whether physiological levels of WT1 expression in some healthy tissues results in the deletion or tolerance induction of WT1-specific T cells. In this PhD project, TCR gene transfer into purified haematopoietic stem cells (HSCs) was used to study the thymic development of WT1-specific T cells and their fate in the periphery. Lentiviral constructs containing the genes for an HLAA2 allorestricted, murinised WT1 TCR or the genes for a control, viral peptide-specific, LMP2 TCR, were generated. The conditions for lentivirally-transduced HSC transplants were optimised. The results obtained from WT1 TCR tranduced HSC transplants in HLA-A2Kb transgenic mice demonstrated that thymocytes expressing this high-avidity WT1 TCR were positively selected into CD8 T cells and emerged in the recipient’s periphery. WT1-specific T cells exhibited a memory, CD44hi phenotype correlating with rapid antigen specific killing, proliferation and cytokine secretion of WT1-specific T cells in the absence of vaccination. LMP2-specific T cells exhibited a naive-like, CD44low phenotype without any antigen specific function. WT1-specific T cells persistent long-term in the periphery of transplanted mice, and no autoimmunity was noted. The results presented in this thesis show for the first time that T cell specificity for a tumour-associated, self-antigen did not result in tolerance induction, but instead mediated the spontaneous generation of functionally competent, memory phenotype T cells

Wavelength tuning of polymer optical fibre Bragg grating at longer wavelengths permanently

Permanent Bragg wavelength tuning of polymer optical fibre Bragg grating (POFBG) at longer wavelengths has been demonstrated for the first time utilising the thermal annealing process. In general, exposing the polymer material above its β-Transition temperature, the fibre shrinks in length, the Bragg grating period becomes shorter and the Bragg wavelength shifts permanently to shorter wavelengths. In this work, a positive tuning of Bragg wavelength has been shown to be feasible when the polymer fibre is stretched during its thermal exposure. The results show that the degree of Bragg wavelength tuning strongly depends on the applying fibre strain or equivalently stress. The work presented in this paper can be used to multiplex POFBGs at any desirable wavelength

Impact of thermal pre-treatment on preforms for fast Bragg gratings inscription using undoped PMMA POFs

In this work, improvements in the photosensitivity of undoped POFs, where there was a welldefined pre-annealing of both preforms in two-step process, were reported. We have noticed that when the primary and secondary preforms are annealed, the fiber photosensitivity is higher; otherwise, if any preform (primary or secondary) is not annealed, the fiber photosensitivity is lower. Two PMMA mPOFs are used where the primary and secondary preforms, during the two-step drawing process, have a different thermal treatment. The PMMA POFs drawn where the primary or secondary preform is not specifically pre-treat need longer inscription time than the fibres drawn where both preforms have been pre-annealed at 80°C for 2 weeks. Using two different UV lasers, for the latter fibre much less inscription time is needed compared to another homemade POF. The properties of a POF fabricated where there are both preform process with thermal treatment is different from those where just one preform step process is thermal treated, as previously shown in the literature, where these POFs are much less sensitive to thermal treatment. Some important parameters were considered such as drawing tension and water content, where using fibers drawn in different tensions give us a similar FBG inscription time

Annealing and etching effects on strain and stress sensitivity of polymer optical fibre Bragg grating sensors

Thermal annealing and chemical etching effects on the strain and stress sensitivity of polymer optical fibre based sensors are investigated. Bragg grating sensors have been photo-inscribed in PMMA optical fibre and their strain and stress sensitivity has been characterised before and after any annealing or etching process. The annealing and etching processes have been tried in different sequence in order to investigate their impact on the sensor’s performance. Results show with high confidence that fibre annealing can improve both strain and stress sensitivities. The fibre etching can also provide stress sensitivity enhancement, however the strain sensitivity changes seems to be random

Polymer optical fiber Bragg grating inscription with a single UV laser pulse

We experimentally demonstrate the first polymer optical fiber Bragg grating inscribed with only one krypton fluoride laser pulse. The device has been recorded in a single-mode poly(methyl methacrylate) optical fiber, with a core doped with benzyl dimethyl ketal for photosensitivity enhancement. One laser pulse with a duration of 15 ns, which provide energy density of 974 mJ/cm2, is adequate to introduce a refractive index change of 0.74×10-4 in the fiber core. After the exposure, the reflectivity of the grating increases for a few minutes following a second order exponential saturation. The produced Bragg grating structure rejects 17.9 dB transmitted power, thus providing 98.4% reflectivity, which is well suited for sensing applications. In addition, we report the importance of the fiber thermal treatment before or after the inscription, showing its effects on the lifetime and quality of the grating structures. Optimizing the irradiation conditions and the material chemical composition, a higher refractive index change in the fiber core is feasible. This demonstration significantly improves the potential for commercial exploitation of the technology

Fiber-optic liquid level monitoring system using microstructured polymer fiber Bragg grating array sensors:performance analysis

A highly sensitive liquid level monitoring system based on microstructured polymer optical fiber Bragg grating (mPOFBG) array sensors is reported for the first time. The configuration is based on five mPOFBGs inscribed in the same fiber in the 850 nm spectral region, showing the potential to interrogate liquid level by measuring the strain induced in each mPOFBG embedded in a silicone rubber (SR) diaphragm, which deforms due to hydrostatic pressure variations. The sensor exhibits a highly linear response over the sensing range, a good repeatability, and a high resolution. The sensitivity of the sensor is found to be 98 pm/cm of water, enhanced by more than a factor of 9 when compared to an equivalent sensor based on a silica fiber around 1550 nm. The temperature sensitivity is studied and a multi-sensor arrangement proposed, which has the potential to provide level readings independent of temperature and the liquid density

Novel thermal annealing methodology for permanent tuning polymer optical fiber Bragg gratings to longer wavelengths

The Bragg wavelength of a polymer optical fiber Bragg grating can be permanently shifted by utilizing the thermal annealing method. In all the reported fiber annealing cases, the authors were able to tune the Bragg wavelength only to shorter wavelengths, since the polymer fiber shrinks in length during the annealing process. This article demonstrates a novel thermal annealing methodology for permanently tuning polymer optical fiber Bragg gratings to any desirable spectral position, including longer wavelengths. Stretching the polymer optical fiber during the annealing process, the period of Bragg grating, which is directly related with the Bragg wavelength, can become permanently longer. The methodology presented in this article can be used to multiplex polymer optical fiber Bragg gratings at any desirable spectral position utilizing only one phase-mask for their photo-inscription, reducing thus their fabrication cost in an industrial setting