Assessing Infrastructure Decisions to Manage Water Resources in the Valle de México

9 p.International audienceGroundwater supplies nearly seventy percent of the regional water supply in Mexico City. Past groundwater extraction in the central city caused subsidence of up to 40 feet in some areas. Today, while pumping in the center city has ceased, groundwater supplies come from areas surrounding Mexico City, which are themselves experiencing effects of groundwater exploitation. Managing groundwater resources to preserve aquifer quality and quantity in the Mexico City Basin must incorporate a variety of strategies, such as promoting infiltration, altering extraction rates, managing demand, and developing wastewater treatment and reuse options. Further, aquifer management decisions have both long-term and short-term planning components. This paper describes a framework for characterizing infrastructure decisions to meet both long-term planning needs and short-term requirements. Long-term decisions include capital-intensive options such as construction of infiltration basins and wastewater treatment facilities, while short-term actions manage demands based on unexpected hydrologic and use parameters. Both short-term and long-term decisions have inherent uncertainty. Such a framework can be used to assess groundwater management and water supply options in Mexico City given infrastructure decisions, water demand forecasts, climate variability, and urban sustainability initiatives. The framework informs planning and management models for the basin

Net solar generation potential from urban rooftops in Los Angeles

Rooftops provide accessible locations for solar energy installations. While rooftop solar arrays can offset in-building electricity needs, they may also stress electric grid operations. Here we present an analysis of net electricity generation potential from distributed rooftop solar in Los Angeles. We integrate spatial and temporal data for property-level electricity demands, rooftop solar generation potential, and grid capacity constraints to estimate the potential for solar to meet on-site demands and supply net exports to the electric grid. In the study area with 1.2 million parcels, rooftop solar could meet 7200 Gigawatt Hours (GWh) of on-site building demands (~29% of demand). Overall potential net generation is negative, meaning buildings use more electricity than they can produce. Yet, cumulative net export potential from solar to grid circuits is 16,400 GWh. Current policies that regulate solar array interconnection to the grid result in unutilized solar power output of 1700 MW. Lower-income and at-risk communities in LA have greater potential for exporting net solar generation to the grid. This potential should be recognized through investments and policy innovations. The method demonstrates the need for considering time-dependent calculations of net solar potential and offers a template for distributed renewable energy planning in cities

Evaluating the effects of turf-replacement programs in Los Angeles

Water utilities incentivize turf replacement to promote water conservation, but the effects of such programs have received limited evaluations. In 2014, the Metropolitan Water District of Southern California (MWD) undertook an unprecedented investment to incentive turf replacement throughout Southern California in response to a serious Statewide drought. MWD devoted $350 million to the program, resulting in more than 46,000 rebate payments (25,000 in Los Angeles County) to remove 15.3 million square meters of turf. The program implementation provided a unique opportunity to address research gaps on turf replacement implementation. We analyzed socioeconomic and spatial trends of program participants and assessed landscape changes from turf replacement using a random sample of properties (4% of LA County participants in 2014–16). Specifically, we used a novel and cost-effective approach Google Earth Street View to characterize landscapes in front yards and created a typology of land cover types. Results showed: post-replacement landscapes had a diversity of land cover types – diverse yards with several land cover types, as well as more homogenous yards with a single land cover such as woodchips, bare soil, gravel, and artificial turf. Analysis also indicated some evidence of “neighborhood adoption” effects. We describe the need for longitudinal studies to understand long-term effects of turf replacement and associated water use, and suggest that water utilities should also evaluate results in backyards, which requires site visits. This study provides a novel contribution that can be replicated over space and time to further knowledge of turf replacement program implementations and evaluation

Adapting Urban Water Systems to Manage Scarcity in the 21st Century: The Case of Los Angeles.

Acute water shortages for large metropolitan regions are likely to become more frequent as climate changes impact historic precipitation levels and urban population grows. California and Los Angeles County have just experienced a severe four year drought followed by a year of high precipitation, and likely drought conditions again in Southern California. We show how the embedded preferences for distant sources, and their local manifestations, have created and/or exacerbated fluctuations in local water availability and suboptimal management. As a socio technical system, water management in the Los Angeles metropolitan region has created a kind of scarcity lock-in in years of low rainfall. We come to this through a decade of coupled research examining landscapes and water use, the development of the complex institutional water management infrastructure, hydrology and a systems network model. Such integrated research is a model for other regions to unpack and understand the actual water resources of a metropolitan region, how it is managed and potential ability to become more water self reliant if the institutions collaborate and manage the resource both parsimoniously, but also in an integrated and conjunctive manner. The Los Angeles County metropolitan region, we find, could transition to a nearly water self sufficient system

De privilegerede

Genstande kan være ledetråde til for længst glemte historier. Med udgangspunkt i en brudekiste, der er gået i arv i generationer af privilegerede fribønder, kromænd og deres hustruer, undersøger historiker Leah Porse Rasmussen denne gruppes standsbevidsthed og identitet. Selvom de var privilegerede med kongegivne friheder, befandt de sig juridisk set i bondestanden, men de brugte tegn associeret med adelig værdighed. Således udgjorde denne gruppe i Bov sogn et socialt mellemlag i det slesvigske samfund i 1600- og 1700-tallet

Energy use for urban water management by utilities and Households in Los Angeles

Reducing energy consumption for urban water management may yield economic and environmental benefits. Few studies provide comprehensive assessments of energy needs for urban water sectors that include both utility operations and household use. Here, we evaluate the energy needs for urban water management in metropolitan Los Angeles (LA) County. Using planning scenarios that include both water conservation and alternative supply options, we estimate energy requirements of water imports, groundwater pumping, distribution in pipes, water and wastewater treatment, and residential water heating across more than one hundred regional water agencies covering over 9 million people. Results show that combining water conservation with alternative local supplies such as stormwater capture and water reuse (nonpotable or indirect potable) can reduce the energy consumption and intensity of water management in LA. Further advanced water treatment for direct potable reuse could increase energy needs. In aggregate, water heating represents a major source of regional energy consumption. The heating factor associated with grid-supplied electricity drives the relative contribution of energy-for-water by utilities and households. For most scenarios of grid operations, energy for household water heating significantly outweighs utility energy consumption. The study demonstrates how publicly available and detailed data for energy and water use supports sustainability planning. The method is applicable to cities everywhere

A versatile one-step CRISPR-Cas9 based approach to plasmid-curing

Abstract Background Plasmids are widely used and essential tools in molecular biology. However, plasmids often impose a metabolic burden and are only temporarily useful for genetic engineering, bio-sensing and characterization purposes. While numerous techniques for genetic manipulation exist, a universal tool enabling rapid removal of plasmids from bacterial cells is lacking. Results Based on replicon abundance and sequence conservation analysis, we show that the vast majority of bacterial cloning and expression vectors share sequence similarities that allow for broad CRISPR-Cas9 targeting. We have constructed a universal plasmid-curing system (pFREE) and developed a one-step protocol and PCR procedure that allow for identification of plasmid-free clones within 24 h. While the context of the targeted replicons affects efficiency, we obtained curing efficiencies between 40 and 100% for the plasmids most widely used for expression and engineering purposes. By virtue of the CRISPR-Cas9 targeting, our platform is highly expandable and can be applied in a broad host context. We exemplify the wide applicability of our system in Gram-negative bacteria by demonstrating the successful application in both Escherichia coli and the promising cell factory chassis Pseudomonas putida. Conclusion As a fast and freely available plasmid-curing system, targeting virtually all vectors used for cloning and expression purposes, we believe that pFREE has the potential to eliminate the need for individualized vector suicide solutions in molecular biology. We envision the application of pFREE to be especially useful in methodologies involving multiple plasmids, used sequentially or simultaneously, which are becoming increasingly popular for genome editing or combinatorial pathway engineering