Letter to William Younger regarding the SEAALL Annual Meeting, August 15, 1973

A letter from Adolfo Porrata-Doria to William Younger inviting SEAALL to host a future meeting in Puerto Rico

Letter to William Younger regarding the SEAALL Annual Meeting, August 30, 1973

A letter from Adolfo Porrata-Doria to William Younger discussing Porrata-Doria\u27s plan to invite SEAALL and SEAALS to host a joint meeting in Puerto Rico in 1974

Dehydration tolerance in yeast

La habilidad de las levaduras de superar la deshidratación y de reactivar su metabolismo después de la rehidratación tiene una importancia en la industria de los alimentos y en la biotecnología. Nosotros hemos dirigido nuestro trabajo a mejorar la viabilidad y vitalidad de las levaduras después de la rehidratación. Se realizaron estudios desde el punto de vista fisiológico de las levaduras durante la optimización de las condiciones de rehidratación y estudios moleculares como la determinación de los genes esenciales de respuesta a Secado y Rehidratación (SR) y la caracterización de la muerte celular a consecuencia del SR. Se sobre expresaron genes que codifican péptidos que permiten superar la viabilidad alcanzada por las levaduras bajo estas condiciones de estrés.Hipótesis de partida:Algunos metabolitos y genes esenciales de respuesta a estrés por secado y rehidratación permiten a las levaduras tolerar la desecaciónThe ability of yeast to overcome dehydration and restart metabolism after rehydration has an importance in the food industry and biotechnology. We have directed our work to improve the viability and vitality of the yeast after rehydration. The studies were conducted in one hand from the physiological point of view to optimize rehydration conditions, and in the other hand from the molecular point of view. We identified the essential genes in response to drying and rehydration and its role in yeast cell death. Moreover we study the effect of over expressed some of this genes on yeast desiccation tolerance.Hypothesis: Some metabolites and essential genes in response to stress during drying and rehydration allow yeasts tolerate desiccation

Design and Optimization of a Mycoplasma Detection Assay

Mycoplasma are among the smallest free living microorganisms. These bacteria grow slowly, lack a rigid cell wall and are not eliminated by filter sterilization methods used in tissue culture. Mycoplasma infection affects biochemical and genetic aspects of cultured cells, resulting in experimental inconsistency. Therefore, it is necessary to establish routine testing for mycoplasma contamination in tissue culture laboratories. Our goal is to develop a reliable and cost-effective test for mycoplasma in cell culture based on established methods found in literature. We first cloned and sequenced a PCR product from a commercial mycoplasma detection kit. Sequencing revealed the 16s rRNA as the target for mycoplasma detection; we confirmed this target by conducting a literature search. PCR primers were designed using 16s rRNA gene as a target. We set-up reactions and optimized conditions for the real-time PCR assay to detect the target and confirmed amplicon size with agarose gel electrophoresis. We identified that 56oC was the best temperature for the PCR and found that agarose gel electrophoresis was a better detection method because it identified the size to confirm the proper product. The primers we ordered to develop this assay produce the proper band; however, results of several assays have been inconsistent as sometimes a known positive sample fails to amplify. As well, in several PCR reactions the negative showed a signal. The overall reaction needs improvements to have greater reliability and to eliminate all sources of contamination. Research is continuing results are not final

Resolving the Crowdfunding Conundrum: The Experience of the United States and Spain

The phenomenon known as crowdfunding has become an attractive alternative for businesses looking for investors without having to go through more well-established routes or without necessarily having to lure and impress professional investors. However, this new form of raising capital creates a series of issues and problems unique to crowdfunding, which has led to a struggle amongst governments to effectively regulate this new entrepreneurial opportunity. The crowdfunding conundrum government regulators are facing causes them to have to reconcile two contradictory missions: facilitating the acquisition of capital by businesses and protecting investors (and the market) from fraud and manipulation. This Article analyzes this conundrum from a United States (“U.S.”) and Spanish perspective. I first describe the crowdfunding conundrum in general terms by explaining how crowdfunding (both consumer and accredited investor) works in practice and explore the major problems and issues that startup companies, investors, the market, and the state face in crowdfunding, which need to be resolved in a regulatory system. I will then describe and evaluate the current American and Spanish and proposed European regulatory solutions to the crowdfunding conundrum. I then conclude by evaluating whether and how well the United States’ and Spain’s regulations, as well as the European Union’s (“EU”) proposed regulations, have attempted to resolve the conundrum by balancing the risks and problems facing crowdfunding transactions

ALBA AND UNASUR: BACK TO THE FUTURE?

This essay discusses efforts at creating a unified Latin American region through the lens of different integration attempts. Part I briefly examines MERCOSUR and the Andean Group and how these two efforts failed to achieve promises made under the free trade model that grew under the Washington Consensus. Structural problems and changing political tides left these two groups unsuccessful, and ultimately the election of populist leftwing presidents in Argentina, Brazil, and Venezuela ushered in a new model of integration intended to increase the economic development of Latin America in an equitable fashion. Despite their different ideologies and missions, both Alianza Bolivariana de las Américas (“ALBA”) and Unión Suramericana de Naciones or Union of South American Nations (“UNASUR”) shared much with their predecessors. Part II describes the first of these new efforts, ALBA, tracing its history, development, organizational structure, institutions, grannational enterprises, and bank, creating a picture of ALBA’s failure over time. ALBA sought the transformation of Latin American societies, making them more just, participative, and united, through the enactment of various principles and a general framework. The essay also explains the ways ALBA leadership attempted to refine goals over time with little success. This section includes a discussion of PETROCARIBE, an agreement signed at a summit of Caribbean nations, and how this treaty diluted ALBA’s goals. Part III examines UNASUR tracing its origins, mission, organizational structure, institutions, and specialized councils. UNASUR was modeled after the European Union and sought to establish full economic, political, and monetary unity in South America. This section outlines several issues that prevented UNASUR from achieving its goals, such as differing political agendas amongst member states and major structural issues, to show how UNASUR, like ALBA, ultimately failed its mission. In particular, this section explicates the various councils enacted by UNASUR and reveals how their structure and lack of institutional framework made them ineffective. Part IV concludes that the failures of both ALBA and UNASUR to achieve their goals or even to survive underscores several important lessons for integration organizations

Resolving the Crowdfunding Conundrum: The Experience of the United States and Spain

The phenomenon known as crowdfunding has become an attractive alternative for businesses looking for investors without having to go through more well-established routes or without necessarily having to lure and impress professional investors. However, this new form of raising capital creates a series of issues and problems unique to crowdfunding, which has led to a struggle amongst governments to effectively regulate this new entrepreneurial opportunity. The crowdfunding conundrum government regulators are facing causes them to have to reconcile two contradictory missions: facilitating the acquisition of capital by businesses and protecting investors (and the market) from fraud and manipulation. This Article analyzes this conundrum from a United States (“U.S.”) and Spanish perspective. I first describe the crowdfunding conundrum in general terms by explaining how crowdfunding (both consumer and accredited investor) works in practice and explore the major problems and issues that startup companies, investors, the market, and the state face in crowdfunding, which need to be resolved in a regulatory system. I will then describe and evaluate the current American and Spanish and proposed European regulatory solutions to the crowdfunding conundrum. I then conclude by evaluating whether and how well the United States’ and Spain’s regulations, as well as the European Union’s (“EU”) proposed regulations, have attempted to resolve the conundrum by balancing the risks and problems facing crowdfunding transactions