735 research outputs found
Constraining the Distribution of L- & T-Dwarfs in the Galaxy
We estimate the thin disk scale height of the Galactic population of L- &
T-dwarfs based on star counts from 15 deep parallel fields from the Hubble
Space Telescope. From these observations, we have identified 28 candidate L- &
T- dwarfs based on their (i'-z') color and morphology. By comparing these star
counts to a simple Galactic model, we estimate the scale height to be 350+-50
pc that is consistent with the increase in vertical scale with decreasing
stellar mass and is independent of reddening, color-magnitude limits, and other
Galactic parameters. With this refined measure, we predict that less than 10^9
M_{sol} of the Milky Way can be in the form L- & T- dwarfs, and confirm that
high-latitude, z~6 galaxy surveys which use the i'-band dropout technique are
97-100% free of L- & T- dwarf interlopers.Comment: 4 pages, 4 figures, accepted to ApJ
The Biostimulant, Potassium Humate Ameliorates Abiotic Stress in Arabidopsis thaliana by Increasing Starch Availability
[EN] Potassium humate is a widely used biostimulant known for its ability to enhance growth and improve tolerance to abiotic stress. However, the molecular mechanisms explaining its effects remain poorly understood. In this study, we investigated the mechanism of action of potassium humate using the model plant Arabidopsis thaliana. We demonstrated that a formulation of potassium humate effectively increased the fresh weight accumulation of Arabidopsis plants under normal conditions, salt stress (sodium or lithium chloride), and particularly under osmotic stress (mannitol). Interestingly, plants treated with potassium humate exhibited a reduced antioxidant response and lower proline accumulation, while maintaining photosynthetic activity under stress conditions. The observed sodium and osmotic tolerance induced by humate was not accompanied by increased potassium accumulation. Additionally, metabolomic analysis revealed that potassium humate increased maltose levels under control conditions but decreased levels of fructose. However, under stress, both maltose and glucose levels decreased, suggesting changes in starch utilization and an increase in glycolysis. Starch concentration measurements in leaves showed that plants treated with potassium humate accumulated less starch under control conditions, while under stress, they accumulated starch to levels similar to or higher than control plants. Taken together, our findings suggest that the molecular mechanism underlying the abiotic stress tolerance conferred by potassium humate involves its ability to alter starch content under normal growth conditions and under salt or osmotic stress.This research was funded by the CDTI program project EXP 00137666/IDI-20210456. awarded to CALDIC Ibérica S.L. and the research contract. "DESARROLLO DE FORMULADOS BIOESTIMULANTES Y BIOFERTILIZANTES INNOVADORES DE ORIGEN NATURAL (CALBIO) DESTINADOS A LA AGRICULTURA CONVENCIONAL Y ECOLÓGICA. ESTUDIO CIENTÍFICO DE EFECTOS SINÉRGICOS ENTRE BIOACTIVOS MICROBIANOS Y NO MICROBIANOS" Between CALDIC Ibérica S.L. and Universitat Politècnica de València. The APC was funded by the aforementioned research contract.Benito, P.; Bellón, J.; Porcel, R.; Yenush, L.; Mulet, JM. (2023). The Biostimulant, Potassium Humate Ameliorates Abiotic Stress in Arabidopsis thaliana by Increasing Starch Availability. International Journal of Molecular Sciences. 24(15):1-21. https://doi.org/10.3390/ijms241512140121241
Overexpression of BvHb2, a Class 2 Non-Symbiotic Hemoglobin from Sugar Beet, Confers Drought-Induced Withering Resistance and Alters Iron Content in Tomato
[EN] Drought stress is one of the major threats to agriculture and concomitantly to food production. Tomato is one of the most important industrial crops, but its tolerance to water scarcity is very low. Traditional plant breeding has a limited margin to minimize this water requirement. In order to design novel biotechnological approaches to cope with this problem, we have screened a plant cDNA library from the halotolerant crop sugar beet (Beta vulgaris L.) for genes able to confer drought/osmotic stress tolerance to the yeast model system upon overexpression. We have identified the gene that encodes BvHb2, a class 2 non-symbiotic hemoglobin, which is present as a single copy in the sugar beet genome, expressed mainly in leaves and regulated by light and abiotic stress. We have evaluated its biotechnological potential in the model plant Arabidopsis thaliana and found that BvHb2 is able to confer drought and osmotic stress tolerance. We also generated transgenic lines of tomato (Solanum lycopersicum) overexpressing BvHb2 and found that the resulting plants are more resistant to drought-induce withering. In addition, transgenic lines overexpressing BvHb2 exhibit increased levels of iron content in leaves. Here, we show that class 2 non-symbiotic plant hemoglobins are targets to generate novel biotechnological crops tolerant to abiotic stress. The fact that these proteins are conserved in plants opens the possibility for using Non-GMO approaches, such as classical breeding, molecular breeding, or novel breeding techniques to increase drought tolerance using this protein as a target.This project was funded by the project PAID-00-10 "Introduccion De Genes Relacionados Con La Tolerancia A Estres Hidrico Y Oxidativo En Distintos Materiales Que Presentan Caracteristicas Utiles Para Su Uso Como Patrones De Plantas Horticolas De Interes Agronomico". 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BvCOLD1: A novel aquaporin from sugar beet (Beta vulgaris L.) involved in boron homeostasis and abiotic stress
[EN] In this report we have identified BvCOLD1, a novel aquaporin from sugar beet (Beta vulgaris) which is only conserved in the Chenopodioideae family. BvCOLD1 is expressed in all plant organs investigated and located in the endoplasmic reticulum. Transport experiments in yeast indicated that BvCOLD1 is able to transport glycerol and boron, the most limiting oligoelement for sugar beet cultivation. Overexpression of BvCOLD1 in Arabidopsis thaliana plants conferred tolerance to cold, to different abiotic stresses and the ability to grow under boron limiting conditions, therefore this novel aquaporin may be an important target to design new crops with enhanced boron homeostasis and abiotic stress tolerance.Ministerio de Economia y Competitividad, Grant/Award Number: BIO2016-77776-P; Secretaria de Estado de Investigacion, Desarrollo e Innovacion, Grant/Award Number: AGL2013-47886-R; Direccion General Investigacion Cientifica; MINECO, Grant/Award Numbers: BIO2014-61826 and BIO2016-77776-P; Universitat Politecnica de Valencia, Grant/Award Number: PAID-06-10-1496Porcel, R.; Bustamante-González, AJ.; Ros, R.; Serrano Salom, R.; Mulet, JM. (2018). BvCOLD1: A novel aquaporin from sugar beet (Beta vulgaris L.) involved in boron homeostasis and abiotic stress. 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Effects of growth conditions of donor plants and in vitro culture environment in the viability and the embryogenic response of microspores of different eggplant genotypes
[EN] Notwithstanding the importance of eggplant in global horticulture, doubled haploid production in this species is still far from being efficient. Although acknowledged to have a role in the efficiency of androgenesis induction, factors such as the growth conditions of donor plant or the in vitro culture environment have not been deeply explored or not explored at all in eggplant, which leaves room for further improvement. In this work, we investigated the effects of different in vivo and in vitro parameters on the androgenic performance of different eggplant genotypes, including two hybrids and a DH line. The in vivo parameters included the exposure of donor plants to different temperature and light conditions and to increased levels of boron. The in vitro parameters included the use of different concentrations of NLN medium components, sucrose and growth regulators, and the suspension of microspores at different densities. Our results showed that whereas greenhouse temperature variations or boron application did not to have a positive influence, greenhouse lighting influenced their viability, thereby conditioning the embryogenic response. Changes in different sucrose, salts and hormone levels had different effects in the genotypes studied, which correlated with their genetic constitution. Finally, we determined the best microspore density, different from that previously proposed. Our work shed light on the role of different factors involved in eggplant microspore cultures, some of them not yet studied, contributing to make microspore culture a more efficient tool in eggplant breeding.This work was supported by Grant AGL2017-88135-R to JMSS from Spanish MICINN, respectively, jointly funded by FEDER. ARS and CCF were supported by predoctoral fellowships from the FPI Programs of Universitat Politecnica de Valencia and Generalitat Valenciana, respectively.Rivas-Sendra, A.; Corral Martínez, P.; Camacho-Fernández, C.; Porcel, R.; Seguí-Simarro, JM. (2020). Effects of growth conditions of donor plants and in vitro culture environment in the viability and the embryogenic response of microspores of different eggplant genotypes. Euphytica. 216(11):1-15. https://doi.org/10.1007/s10681-020-02709-4S11521611Abdollahi MR, Corral-Martinez P, Mousavi A, Salmanian AH, Moieni A, Seguí-Simarro JM (2009) An efficient method for transformation of pre-androgenic, isolated Brassica napus microspores involving microprojectile bombardment and Agrobacterium-mediated transformation. Acta Physiol Plant 31:1313–1317Aulinger IE (2002) Combination of in vitro androgenesis and biolistic transformation: an approach for breeding transgenic maize (Zea mays L.) lines. 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Simvastatin preserves myocardial perfusion and permeability in experimental hypercholesterolemia independent of lipid lowering
Simvastatin preserves myocardial perfusion and coronary microvascular permeability in experimental hypercholesterolemia independent of lipid lowering
AbstractObjectivesThis study was designed to assess the lipid-independent effects of simvastatin on myocardial perfusion (MP) and coronary microvascular permeability index (PI) at baseline and during episodes of increased cardiac demand in experimental hypercholesterolemia.BackgroundSimvastatin preserves coronary endothelial function in experimental hypercholesterolemia independent of its lipid-lowering effect. However, the functional significance of this observation is unknown.MethodsPigs were randomized to three groups: normal diet (N), high-cholesterol diet (HC) and HC diet plus simvastatin (HC+S) for 12 weeks. Subsequently, cardiac electron beam computed tomography was performed before and during intravenous infusion of adenosine and dobutamine, and MP and PI were calculated.ResultsTotal and low density lipoprotein cholesterol levels were similarly and significantly increased in HC and HC+S animals compared with N. Basal MP was similar in all groups. Myocardial perfusion significantly increased in response to either adenosine or dobutamine in N and HC+S animals. Dobutamine also significantly increased MP in HC animals. However, the changes of MP in response to either drug were significantly lower in the HC group compared with the other two groups (p < 0.01 for adenosine and p < 0.05 for dobutamine vs. N and HC+S). Basal PI was similar in all groups and was not altered by either drug in N and HC+S animals. In contrast, PI significantly increased in HC pigs during infusion of either adenosine (p < 0.001) or dobutamine (p < 0.05).ConclusionsThese findings demonstrate that chronic administration of simvastatin preserves myocardial perfusion response and coronary microvascular integrity during cardiac stress in experimental hypercholesterolemia independent of lipid lowering
The Milky Way: An Exceptionally Quiet Galaxy; Implications for the formation of spiral galaxies
[Abridged]We compare both the Milky Way and M31 galaxies to local external
disk galaxies within the same mass range, using their relative locations in the
planes formed by V_flat versus M_K, j_disk, and the average Fe abundance of
stars in the galaxy outskirts. We find, for all relationships, that the MW is
systematically offset by ~ 1 sigma, showing a significant deficiency in stellar
mass, in angular momentum, in disk radius and [Fe/H] in the stars in its
outskirts at a given V_flat. On the basis of their location in the M_K, V_flat,
and R_d volume, the fraction of spirals like the MW is 7+/-1%, while M31
appears to be a "typical'' spiral. Our Galaxy appears to have escaped any
significant merger over the last ~10 Gyrs which may explain why it is deficient
by a factor 2 to 3 in stellar mass, angular momentum and outskirts metallicity
and then, unrepresentative of the typical spiral. As with M31, most local
spirals show evidence for a history shaped mainly by relatively recent merging.
We conclude that the standard scenario of secular evolution is generally unable
to reproduce the properties of most (if not all) spiral galaxies. However, the
so-called "spiral rebuilding'' scenario proposed by Hammer et al. 2005 is
consistent with the properties of both distant galaxies and of their
descendants - the local spirals.Comment: 14 pages, 6 figures, to appear in Ap
Biological Control of Three Fungal Diseases in Strawberry (Fragaria X ananassa) with Arbuscular Mycorrhizal Fungi
[EN] Similar to many other plant-based products, strawberries are susceptible to fungal diseases
caused by various pathogen groups. In recent years, efforts have been made to combat these
diseases using biological control methods, particularly the application of arbuscular mycorrhizal
fungi (AMF). This study aimed to determine the effects of AMF (Funneliformis mosseae (Fm) and
Gigaspora margarita (Gm)) on Rhizoctonia fragariae (Rf), Fusarium oxysporum (Fo), and Alternaria alternata
(Aa), which are major pathogens for strawberry. The results showed that the effects of AMF on
disease severity and plant growth varied depending on the pathogens involved. Rf caused the
highest disease severity, followed by Fo and Aa, but all AMF treatments significantly reduced the
disease severity compared to control treatments. The study also found that the specific AMF species
and their combinations influenced plant growth responses under different pathogenic conditions.
Different AMF treatments resulted in varying increases in plant fresh weight, dry weight, and length,
depending on the pathogen. Moreover, the application of AMF led to increased levels of total
phenolic content, antioxidant activity, and phosphorus content in pathogen-infected plants compared
to control treatments. Fm was more efficient than Gm in increasing these biochemical parameters.
The levels of root colonization by AMF were similar among different AMF treatments, but the effects
on fungal spore density varied depending on the pathogen. Some AMF treatments increased fungal
spore density, while others did not show significant differences. In conclusion, our research sheds
light on the differential effects of AMF species on disease severity, plant growth, and biochemical
parameters in strawberry plants facing diverse pathogens. These findings underscore the potential
benefits of AMF in disease management, as they reduce disease severity and bolster plant growth
and defense mechanisms.This research study was financially supported by the Scientific Research Projects Coordination Unit of Van Yuzuncu Yil University. Project number: FBA-2019-7833.Demir, S.; Durak, ED.; Günes, H.; Boyno, G.; Mulet, JM.; Danesh, YR.; Porcel, R. (2023). Biological Control of Three Fungal Diseases in Strawberry (Fragaria X ananassa) with Arbuscular Mycorrhizal Fungi. Agronomy. 13(9). https://doi.org/10.3390/agronomy1309243913
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