Insight into the Aroma Profile and Sensory Characteristics of ‘Prokupac’ Red Wine Aromatised with Medicinal Herbs

Autochthones grape variety of ‘Prokupac’ (Vitis vinifera L.) is being increasingly cultivated in the Republic of Serbia and is one of the predominant varieties in the vineyards of southern Serbia. ‘Prokupac’ grapes are used to produce red wine with specific and distinctive varietal aromatic characteristics. Medicinal herbs can be effectively combined in alcoholic beverages. The aim of this study was to evaluate the effect of medicinal herbs on the aroma profile and sensory characteristics of ‘Prokupac’ red wine. The analysis of the aromatic composition was conducted on ‘Prokupac’ wine (control) and ‘Prokupac’ wine aromatised with selected medicinal herbs: anise (Pimpinella anisum L.), cinnamon (Cinnamomum verum J. Presl.), wormwood (Artemisia absinthium L.) and licorice (Glycyrrhiza glabra L.). The analysis of volatile aromatic compounds in the wines, performed by the gas chromatography-mass spectrometry (GC/MS) method, identified 48 compounds that were classified in the following groups: alcohols, aldehydes, ketones, acids, ethyl esters and terpenes. Sensory analysis of wines was performed including visual, olfactory, gustatory and gustatory-olfactory perceptions. Terpenes were not identified in the ‘Prokupac’ control wine, while the highest content of all identified aromatic compounds was found in wines aromatised with anise, wormwood and cinnamon. The results indicated that selected medicinal herbs affected the composition and content of volatile aromatic compounds, as well as the sensory characteristics of analyzed wines. The unique aroma profile and pleasant taste of the wine aromatised with cinnamon contributed to its differentiation from other wines, and classification as very good wine

Rapid characterisation of hypanthium and seed in wild and cultivated rosehip: application of Raman microscopy combined with multivariate analysis

Rosehip (pseudo-fruit) of dog rose (Rosa canina L.) is highly valued, and owing to nutritional and sensory properties it has a significant place in the food industry. This work represents an innovative report Focusing on the evaluation of the phytochemical composition of rosehips (hypanthium and seed) grown in different locations in Serbia, using Raman microspectroscopy combined with multivariate data analysis. Some significant differences arose between the analysed rosehip samples with regard to the chemical profile of both hypanthium parenchyma cells and seed, although no evident discrimination was recorded between the samples of wild and cultivated rosehip. The differences between the hypanthium samples compared were mainly determined by the content of carotenoids, phenolic compounds and polysaccharides, whereas phenolics, polysaccharides (pectin, cellulose and hemicellulose) and lipids (to a lower extent) contributed to the seed sample discrimination. The differences observed between the rosehip samples may be attributed to abiotic factors (growing, ripening and storage conditions), which had a significant impact on the carotenoid and polyphenols biosynthesis

Efectiveness of fungal bacterial biofertilizers on agrobiochemical attributes of quinoa (Chenopodium quinoa willd.) under salinity stress

The efects of fungi and bacteria biofertilizers under salinity stress were studied on some morphological and physiological traits of quinoa. The experiment was conducted as a factorial based on a completely randomized design with three replications. The factors included NaCl (no salinity-150 mM NaCl and 300 mM NaCl), Trichoderma (no Trichoderma and Trichoderma), and bacterial biofertilizers (no biofertilizer-N biofertilizer and P biofertilizer). The highest shoot and root length and dry weight were observed in the treatment of no salinity+no Trichoderma+N biofertilizer. Salinity increased Na+and K+content and decreased K+/Na+ratio, whereas the application of Trichoderma alleviated the efect of salinity on these factors. Ca2+/Na+ and Mg2+/Na+ ratios were signifcantly decreased in high salinity level compared to the zero salinity level. The highest and lowest phenol content was observed in the treatments of 300 mM NaCl+Trichoderma+P biofertilizer and no salinity+no Trichoderma+N biofertilizer, respectively. Flavonoid content was higher in the treatments of no salinity+Trichoderma+N biofertilizer and no salinity+Trichoderma+P biofertilizer than in the other treatments, also The high-performance liquid chromatography analysis revealed that all phenolic and favonoid compounds exhibited the greatest increase in treatments of no salinity+Trichoderma+N biofertilizer and no salinity+Trichoderma+P biofertilizer, which is consistent with the signifcant increase in total favonoid content in these treatments. The results showed that using N biofertilizer can improve the growth and development of quinoa in saline soils

Antiproliferative activity of NCI-DTP glutarimide derivatives. An alignment independent 3D QSAR study - Supplementary Material

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Organically vs. Conventionally Grown Vegetables: Multi-elemental Analysis and Nutritional Evaluation

Vegetables are important contributors to a healthy diet, and their adequate daily intake can help prevent some of the major illnesses. The aimof the study was to examine the content of themajor and trace elements in selected organically grown (OG) and conventionally grown (CG) vegetables (cabbage, kohlrabi, Brussels sprout, beetroot, carrot, potato, and onion), taken from city green markets.Multi-elemental analysis was carried out by inductively coupled plasma method with optical emission spectrometry (ICP-OES). Nutritional quality evaluation in comparison to nutritional reference values was done. In studied vegetables, Al, Ca, K, Fe (with the exception of organic kohlrabi),Mg, Na, P, S, and Zn were quantified in all samples, whereas As, Cd, Co, Hg, Se, and V were below the limit of detection for these elements. Macroelements and trace elements were found at higher concentrations in OG and CG vegetables, respectively. Differences in concentrations of studied elements between the same vegetable species produced in two agricultural systems were significant, except for beetroot (p ≤ 0.05). Principal component analysis and hierarchical cluster analysis results showed that the botanical origin had higher influence on sample differentiation than the agronomic practice, which was in accordance with the results obtained by Mann-Whitney U test. Good quality of both OG and CG vegetables in respect of nutritionally beneficial elements was observed

Antiproliferative and antibacterial activity of some glutarimide derivatives

<p>Antiproliferative and antibacterial activities of nine glutarimide derivatives (<b>1</b>–<b>9</b>) were reported. Cytotoxicity of compounds was tested toward three human cancer cell lines, HeLa, K562 and MDA-MB-453 by MTT assay. Compound <b>7</b> (2-benzyl-2-azaspiro[5.11]heptadecane-1,3,7-trione), containing 12-membered ketone ring, was found to be the most potent toward all tested cell lines (IC₅₀ = 9–27 μM). Preliminary screening of antibacterial activity by a disk diffusion method showed that Gram-positive bacteria were more susceptible to the tested compounds than Gram-negative bacteria. Minimum inhibitory concentration (MIC) determined by a broth microdilution method confirmed that compounds <b>1</b>, <b>2</b>, <b>4</b>, <b>6</b>–<b>8</b> and <b>9</b> inhibited the growth of all tested Gram-positive and some of the Gram-negative bacteria. The best antibacterial potential was achieved with compound <b>9</b> (ethyl 4-(1-benzyl-2,6-dioxopiperidin-3-yl)butanoate) against <i>Bacillus cereus</i> (MIC 0.625 mg/mL; 1.97 × 10⁻³mol/L). Distinction between more and less active/inactive compounds was assessed from the pharmacophoric patterns obtained by molecular interaction fields.</p

Antiproliferative and antibacterial activity of some glutarimide derivatives

Antiproliferative and antibacterial activities of nine glutarimide derivatives (1–9) were reported. Cytotoxicity of compounds was tested toward three human cancer cell lines, HeLa, K562 and MDA-MB-453 by MTT assay. Compound 7 (2-benzyl-2-azaspiro[5.11]heptadecane-1,3,7-trione), containing 12-membered ketone ring, was found to be the most potent toward all tested cell lines (IC50 = 9–27 μM). Preliminary screening of antibacterial activity by a disk diffusion method showed that Gram-positive bacteria were more susceptible to the tested compounds than Gram-negative bacteria. Minimum inhibitory concentration (MIC) determined by a broth microdilution method confirmed that compounds 1, 2, 4, 6–8 and 9 inhibited the growth of all tested Gram-positive and some of the Gram-negative bacteria. The best antibacterial potential was achieved with compound 9 (ethyl 4-(1-benzyl-2,6-dioxopiperidin-3-yl)butanoate) against Bacillus cereus (MIC 0.625 mg/mL; 1.97 × 10−3 mol/L). Distinction between more and less active/inactive compounds was assessed from the pharmacophoric patterns obtained by molecular interaction fields