Survey on health status in aquaculture sturgeons centers (Mazandaran, Guilan And Golestan Provinces)

Study of survey health management and diseases in hatcheries and fish farms can help us to knowledge and application control methods such as: prevention, treatment and increase high levels of production in hatchery and farms, finally. This survey carried out from 2005 to 2008 for 4 years in sturgeon hatcheries and farms of Golestan province. Sturgeon fishes include Huso Huso, Ship sturgeon, Acipenser persicus collected and for virology, bacteriology, fungius and hematology examined. Also, physicochemical parameters measured and recorded in different stages of culture. Results of this study showed that all of samples in virology was negative and did not observe any doubetful causes. In bacteriology CFU was variation from 3/9 ×105 to 6/9×10. The most parasites that detected in this survey was Cocolanus espherolanus, Sceria binopsulus semiarmatus and Amphilina fuliacea that separates from Acipenser Percicus, especially. The results about hematology parameters some important hematological indices of ship sturgeon include: The total RBC for female and mail specimens measured as 5.3±1.5 ×10^5, 4.8±0.5×10^5 per mm^3 respectively. The amount of haematocrit and hemoglobin for female and mail determined: 34.3±2.8, 35±1.4 percent and 10.3±0.9, 8.9±0.8 gr/dl .The MCV: 216.3± 96.2, 736.5± 102.5, MCH: 720.2±309.5, 186±0.7 and MCHC: 30±0.8, 25.5±3.4 percent respectively.The total WBC were (female, male): 21320±1054, 20580±777 per mm^3 and neutrophil: 16.4±2.5, 17±1.4 percent and lymphocyte: 74.4±2.4, 73.5± 0.7 percent and eosinophil: 6±1.4, 6.4±0.5 percent, monocyte: 2.8±0.8, 3.5±0.7 percent. There was not any significant differences (p>0.05) between mentioned parameters in male and female (students t-test). Also evaluation of hematological parameters in bluga ( Huso huso) include: total RBC were (male , female) 5±0.3 ×105 , 4.9±0.6 ×105 per mm^3 ,respectively and hematocrit: 33.2±6.7 , 35.4±3.4 percent and hemoglobin: 11.2±1.5 , 12.2±1gr/dl and MCV: 669.9±172.2, 723.9±982.4 and MCH: 226.2±42.5, 249.5±35.4 and MCHC: 34.1±2.4, 34.6±3.6 percent respectively. The totals WBC were (male, female): 24800±707.1, 23042±1375.4 per mm^3 and neutrophil: 18.5±0.7, 21.4±1.1 percent and lymphocyte: 73.5±1.4, 68.4±1.1 percent and eosinophil: 5±2.8, 7±1.2 percent and monocyte: 3.5±3.5, 3.2±0.8 percent. According to statistically study the count of lymphocyte had significant difference between male and female fish and this count in male was higher than female. (p≥0.05)

Detection of Escherichia coli, Salmonella enterica and shigella Dysenteriae by analysis of 23s ribosomal DNA gene

Background: Ribosomal DNA (rDNA) genes contain signature structures which are unique for groups of organisms. Considering their great number in cells and their protected areas, they render ideal targets for specific nucleic acid probes. The present study aimed to investigate the capability of some specific regions of 23S rDNA gene as a DNA target for differentiation and screening of Escherichia coli, Salmonella enterica, and Shigella dysenteriae. Methods: Bacterial reference strains used in this study were E. coli, S. enterica and Sh. dysenteriae that were provided by the centers for microbial culture collection (CMCC) at Pasture Institute of Iran. DNA extraction was performed by boiling method. Alignment of the 23S rDNA sequences of bacterial species was performed by using AlignX (a component of Vector NTI Advance 11.0) and areas displaying sequence divergence among species were used for designing universal primers and individual bacteria specific probe. Findings: The universal polymerase chain reaction (PCR) products of each bacterial species showed bands of approximately 880 bp to be being equivalent to the fragment size of 23S rDNA gene. Different size bands of 23S rDNA probes were produced and included 228 bp for E. coli, 444 bp for S. enterica, and 776 bp for Sh. dysenteriae. Conclusion: Comparative sequence analysis of variable and specific regions of 23S rDNA genes among the studied bacterial species showed that we were able to amplify specific target among universal region for the detection of many enteric pathogenic bacteria

Pre-menopausal vulvar melanoma (Case Report)

Vulvar melanoma is a very rare tumor with the rate of 0.1-0.19/100000. It mostly occurs during post menopausal period. In this case report, our patient was a 43 years old woman, her symptom was a small pruritic lesion noticed two months earlier when she was referred to the clinic. This lesion was a 5mm diameter dark brown and flat on her left labialis minor. Malignant melanoma with 3-5mm thickness was diagnosted following biopsy. Wide local excision with more than 2cm free margin and inguino femoral lymphadenectomy at the site of the lesion was done. The recurrence of tumor was not abserved during four months follow-up