Novel Drug Delivery Systems of Resveratrol to Bioavailability and Therapeutic Effects

Resveratrol is a naturally occurring product used in the prevention and treatment of various diseases by acting as a potent defensive antioxidant. Resveratrol can be used in various fields, but the use is limited due to its poor solubility and hence low bioavailability. For overcoming this limitation, various drug delivery systems of resveratrol were developed. The aim of the novel drug delivery system (NDDS) is to provide a therapeutic amount of drug to the target site to maintain the desired drug concentration. NDDS enhances the duration of therapeutic activity, increases plasma half-life, decreases the immunogenicity, increases the stability of biopharmaceuticals, improves the solubility of low molecular weight drugs so does the bioavailability, and has a potential of targeted drug delivery. However, they have their own advantages as well as limitations. This chapter focuses on: (1) general introduction to resveratrol and its various therapeutic uses, (2) pharmacokinetic- and bioavailability-related problems of resveratrol, and (3) general about various NDDS used in resveratrol formulations

Effect of Seed Priming with Some Plant Leaf Extract on Seedling Growth Characteristics and Root Rot Disease in Tomato

Tomato is one of the important vegetable crops. The problem of seedling establishment is found in tomato due to several soil borne diseases. One of them is root rot caused by Fusarium oxysproum. There are many chemical methods available to control this disease, but use of chemicals deplete the soil micro-environment and causes soil and water pollution and also do not fit within the framework of ‘Organic farming’. Seed priming with certain phytochemicals may be an economic and ecofriendly alternative to such chemicals. In present study we primed tomato seeds with leaf extract of six different plants (White musale, Periwinkle, Neem, Wood apple, Lantana and White cedar). Different leaf extracts of dose of 2% was taken independently for seed priming. We found that priming with White musale, Periwinkle, Neem and wood apple leaf extract had an improvement in different seed and seedling growth parameters in presence of pathogen. Priming with Lantana and white cedar leaf extract showed a reduction in some of the parameters that may be due to allelopathic nature of these plants. Seed priming with leaf extract of Wood apple exhibited maximum survival rate (76.50 %) followed by Neem (68.46 %) and White Musale (52.60 %)

NOVEL RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF SAXAGLIPTIN AND GLIMEPIRIDE

Objective: The objective of the present study was to develop, and validate a novel reverse phase high performance liquid chromatographic (RP-HPLC) method, for simultaneous determination of saxagliptin (SAXA) and glimepiride (GLIM), in bulk mixtures, and in tablets.Methods: Determination of the drugs, SAXA and GLIM, was carried out employing ODS C18 column (250 mm X 4.6 mm i. d, 5 µm particle size), with diode array detector at λmax of 230 nm. The mobile phase employed for the current study, composed of two solvents, i.e., A (acetonitrile), and B (0.1 % w/v sodium di-hydrogen orthophosphate buffer, pH 3.8 adjusted with orthophosphoric acid). The mobile phase was pumped at a flow rate of 0.75 ml/min in the gradient mode. The validation study with respect to specificity, linearity, precision, accuracy, robustness, limit of detection (LOD), and limit of quantification (LOQ), was carried out employing the ICH Guidelines.Results: The developed method was selective and linear for both the drugs, i.e., between 15.63 µg/ml and 250.00 µg/ml for SAXA, and 7.81 µg/ml and 125.00 µg/ml for GLIM, with a correlation coefficient (R2) 0.9977 and 0.9982, for SAXA, and GLIM, respectively. The % recovery obtained was 102.98±0.14% for SAXA, and 101.84±1.96% for GLIM. The LOD and LOQ values for SAXA were obtained to be 1.30 µg/ml, and 3.94 µg/ml, respectively, while for GLIM, LOD was 0.82 µg/ml and LOQ was 2.48 µg/ml. The method also exhibits good robustness for different chromatographic conditions like wavelength, flow rate, mobile phase and injection volume.Conclusion: The method was successfully employed, for the quantification of SAXA and GLIM, in the quality control of in-house developed tablets, and can be applied for the industrial use

Energetics and GHG Emission Mitigation Potential Estimation of Solar Water Heating System in India

The aim of this study is focused on “energetics” or energy analysis and evaluation of greenhouse gas (GHG) mitigation potential of solar water heating system (SWHS) having 100 litre per day (lpd) capacity in 28 states of India. Different types of collectors are used in solar water heating system, which may affect on the feasibility of the system. So to analyze this factor, the present approach focused on three types of glazed flat plate collectors. In addition to this, the feasibility of the system also does depend upon the end user applications. So to incorporate more feasible analysis, three different scenarios were considered. For the analysis of environmental impact of the system, GHG emission mitigation potential has also been evaluated by assuming the replacement of the system with frequently used fuels, viz., natural gas, coal, diesel, etc. This was done for both seasonal and annual use of this renewable energy thermal device. The result of this study shows that energy payback period (EPBP) and energy yield ratio (EYR) values for selected locations are in the adoption level. In case of coal, the GHG-emission reduction was found to have a maximum value of 22.4 tCO2 for the state of Rajasthan and a minimum value of 13.4 tCO2, for Jammu & Kashmir, for 20 years of useful lifetime of the system

Efficacy of resveratrol encapsulated microsponges delivered by pectin based matrix tablets in rats with acetic acid-induced ulcerative colitis

Objectives: The objective of the present work to encapsulate the resveratrol (RES) inside the chitosan-based microsponges, employing the systematic optimization by 33 Box-Behnken design for the colonic targeting.Significance: Enhanced therapeutic efficacy of RES-loaded microsponges and matrix tablets, vis-a-vis pureRES for ulcerative colitis.Methods: RES-loaded microsponges were prepared employing the systematic optimization by 33 Box-Behnken design for the colonic targeting. The best-optimizedRES-loaded microsponge was compressed in the form of a tablet, employing pectin as a matrix-forming material. The encapsulation of RES inside microsponge was confirmed by XRD, DSC and FT-IR. Further, both RES-loaded microsponges and matrix tablets were evaluated for in vitro release kinetics and further evaluated for in vivo ulcerative colitis animal model.Results: Optimization experiments was obtained as the high value of r2 (particle size = 0.9999; %EE = 0.9652; %CDR = 0.9469) inferred excellent goodness of fit. SEM revealed nearly spherical and porous nature of RES-loaded microsponges. The in vitro release kinetic showed zero-order release for RES-loaded microsponges and Korsmeyer-Peppas model for matrix tablets. The pharmacodynamic studies, in ulcerative colitis rat model, indicated better therapeutic efficacy of drug-loaded microsponges and matrix tablets, vis-a-vis pure RES. Thus, the present study advocates the potential of RES based microsponges delivered by pectin based matrix tablet, in the treatment of various colonic disorders.Conclusion: The present study proved that RES-loaded microsponges and matrix tablets based on chitosan and pectin can be the ideal delivery system for colonic delivery of RES

Innovation in traditional organic nutrient management practices for better soilhealth and higher productivity of brahmi (Bacopa monnieri L.)

This study was conducted at Medicinal Plants Research and Development Centre (MRDC) of Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, India, to examine the effect of Jeevamrit (Bioenhancer) on soil health and herbage yield of brahmi crop (var. CIM- Jagriti) and to optimise its rate of application. The experiment was laid out in randomised block design (RBD), replicated trice. The experimental soil was sandy clay loam in texture, neutral in reaction, having pH 6.9, medium in organic carbon (0.64%), low in available nitrogen (180.78 kg ha-1) and medium in both available phosphorus (20.14 kg ha-1) and potassium (200.64 kg ha-1). Jeevamrit enhances soil nutrient content, improve bulk density and biodiversity by increasing beneficial soil microbes which mineralise the nutrients present in soil and increase their availability. Jeevamrit 4000 l ha-1 can be used as a nutrient source in place of chemical fertilisers and expensive bulky organic manures under organic nutrient management

Quantification of Parasite Load in Clinical Samples of Leishmaniasis Patients: IL-10 Level Correlates with Parasite Load in Visceral Leishmaniasis

A rapid and accurate method to detect and quantify Leishmania parasite is urgently needed to facilitate early diagnosis of Leishmaniasis and monitoring of antileishmania therapy. In this study, real-time assay was applied to estimate parasite load in clinical samples of visceral leishmaniasis (VL) and post kala-azar dermal leishmaniasis (PKDL) patients. The mean parasite load in blood of VL patients (n = 31) was 8,372 parasites/ml, while the mean parasite load in bone marrow aspirate (BMA) was 194,962 parasites/million nucleated cells (n = 12). Parasite load was undetectable after treatment with amphotericin B (n = 9) in VL, while a residual parasite burden was detected in 2 of 6 patients following treatment with sodium antimony gluconate. Further, circulating levels of IFN-γ, TNF-α, IL-10, IL-6, IL-4 and IL-2 were analysed in VL patients (n = 29) by Cytometric Bead Array to evaluate correlation with parasitic load. Interestingly, IL-10 levels correlated significantly with parasite load (r = 0.82, P<0.0001). The mean parasite load in dermal lesions of PKDL patients was 9,502 parasites/µg tissue DNA at pre-treatment stage (n = 25), with no detectable parasites after therapy (n = 5). Parasite burden was distinctly higher (P<0.0001) in nodular lesions (n = 12) (19,586 parasites/µg tissue DNA) compared to papular/macular lesions (n = 13, 193 parasites/µg tissue DNA). Further, chronic PKDL lesions showed significantly (P = 0.0166) higher parasite load in comparison with acute lesions. Results indicate that chronic, nodular cases constitute the major parasite reservoir for anthroponotic transmission. Our results establish that the high parasite load in VL is strongly correlated with a high level of IL-10, implicating IL-10 as a marker of disease severity. The assay is applicable for diagnosis as well as prognosis of both VL and PKDL, providing a simple molecular tool to monitor the efficacy of antileishmanial drugs or vaccines

Nanocarriers: more than tour de force for thymoquinone

Introduction: Thymoquinone (TQ), 2-isopropyl-5-methylbenzo-1, 4-quinone, the main active constituent of Nigella sativa (NS) plant, has been proven to be of great therapeutic aid in various in vitro and in vivo conditions. Despite the promising therapeutic activities of TQ, this molecule is not yet in the clinical trials, restricted by its poor biopharmaceutical properties including photo-instability.Area covered: This review compiles the different types of polymeric and lipidic nanocarriers (NCs), encapsulating TQ for their improved oral bioavailability, and augmented in vitro and in vivo efficacy, evidenced on various pathologies. Furthermore, we provide a comprehensive overview of TQ in relation to its encapsulation approaches advancing the delivery and improving the efficacy of TQ.Expert opinion: TQ was first identified in the essential oil of Nigella sativa L. black seed. TQ has not been used in formulations because it is a highly hydrophobic drug having poor aqueous solubility. To deal with the poor physicochemical problems associated with TQ, various NCs encapsulating TQ have been tried in the past. Nevertheless, these NCs could be impending in bringing forth this potential molecule to clinical reality. This will also be beneficial for a large research community including pharmaceutical & biological sciences and translational researchers

Emerging trends in clinical implications of bio-conjugated silver nanoparticles in drug delivery

© 2020 Elsevier B.V. From nanopharmaceutics to renewable energy, silver nanoparticles (AgNPs) present innumerable applications in the contemporary era. However, the associated toxicity to the biosystems limits their application. Effective utilization of AgNPs, therefore, requires their surface conjugation with biologically benevolent moieties that enhance the bio-acceptability of silver-based nanosystems, and supplementary functionalities for further extension of their unique applications. The clinical importance of AgNPs was established long ago, but their clinical utilization has been explored only recently with the phenomenon of bio-conjugation. The biomolecule-conjugated AgNPs present operable solutions for tedious clinical complications of the present era, such as multidrug resistance, designing of pharmaceuticals with improved bioavailability, superior drug delivery vehicles and in situ bio imaging of important metabolites that utilize the biomolecule-anchored surface engineered AgNPs. This review epigrammatically discusses some interesting clinical applications of surface conjugated AgNPs with biomolecules such as peptides, nucleic acids, amino acids and antibodies in the current nanopharmaceutical paradigm