15 research outputs found

    Calcium carbonate dissolution rates in hydrothermal vent fields of the Guaymas Basin

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    Analysis of bivalve shell fragments that were embedded in epoxy blocks, mounted on titanium stakes, and deployed by DSRV Alvin at 5 sites in the Southern Trough of the Guaymas Basin (27°00′N, 111°24.55′W; depth 2012 m) indicates significant variation of calcium carbonate dissolution in in situ exposures of more than 900 days. Arrays of shell fragments of six bivalve species (i.e., Bathymodiolus thermophilus, Calyptogena magnifica, Calyptogena sp., Corbicula fluminea, Crassostrea virginica and Mytilus edulis) were positioned −17 cm, −7 cm and −2.5 cm below the sediment-water interface and 2.5 cm, 7 cm and 17 cm above the sediment-water interface in hydrothermal vent fields of the basin. Maximum dissolution rates for both calcite (mean = 86 μm/yr) and aragonite (mean = 312 μm/yr) were found in epoxy blocks located at the deepest point sampled in the sediment column (depth = 17 cm). Minimum dissolution rates of calcite and aragonite were found 7 cm (mean = 26 μm/yr) and 2.5 cm (mean = 96 μm/yr) above the sediment-water interface, respectively. Intermediate rates of dissolution were recorded 17 cm above the sediment-water interface (mean = 40 μm/yr for calcite and 126 μm/yr for aragonite). Mean rates of aragonite dissolution ranged from 59 μm/yr (site 5; clam area) to 227 μm/yr (site 3; clam area), and those of calcite dissolution ranged from 13 μm/yr (site 3; clam area) to 94 μm/yr (site 4; bacterial mat area). Dissolution rates were consistently highest in the bacterial mat area (site 4; mean = 94 μm/yr for calcite and 223 μm/yr for aragonite). Rates of calcium carbonate dissolution reported here for hydrothermal vent fields of the Guaymas Basin compare favorably with those of Rose Garden (Galapagos Rift) and 21N (East Pacific Rise) hydrothermal vent sites. These results have important implications for assessing biological rate processes in deep-sea hydrothermal vent environments

    Scanning Electron Microscopic Aids for Identification of Larval and Post-Larval Bivalves

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    The identification of bivalve larvae and early postlarvae in plankton and benthic samples has long been a challenge, hampering both basic and applied research efforts in marine, estuarine, and freshwater environments. The usefulness of published optical micrographs of the early life-history stages of bivalves is limited because of the great morphological similarity of the imaged articulated shells, particularly at the early (straight-hinge) developmental stages. While a number of techniques have been refined in recent years and show promise for use in routine identifications of larval and post-larval bivalves (e.g., single-step nested multiplex polymerase chain reaction; in situ hybridization protocols through color coding with taxon-specific, dye-labeled DNA probes; coupled fluorescence in situ hybridization and cell sorting; and image analysis techniques using species-specific shell birefringence patterns under polarized light), no adequate comprehensive reference source exists that accurately depicts the morphology and morphometry of the shells of larval and post-larval stages of target bivalve species in a consistent format to assist in identification of such stages. To this end, scanning electron micrograph (SEM) sequences are presented of the disarticulated shell valves of laboratory-reared larval and post-larval stages of 56 species of bivalve molluscs from a wide spectrum of marine, estuarine, and freshwater habitats. Emphasis is placed on the usefulness of the morphology and morphometrics of consistently-oriented, disarticulated shell valves and associated hinge structures in discriminating the early life-history stages of these various bivalve species. Although the scanning electron micrograph sequences presented accurately depict the gross morphologies/morphometrics and hinge structures of the disarticulated shell valves of the larvae and/or postlarvae of the 56 species of bivalves, it is important to emphasize that a scanning electron microscope is not necessary to observe even fine hinge structures associated with the early ontogenetic stages of these species. Such structures are readily visible using a wide range of optical compound microscopes equipped with high-intensity reflected light sources, although the disarticulated shell valves must be viewed in several planes of focus to discern the often subtle details seen clearly in the scanning electron micrographs. These morphological characters provide researchers with invaluable aids for the routine identification of the early life-history stages of these species isolated from plankton and benthic samples
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