2 research outputs found
Efecto de dos citoquininas, 谩cido asc贸rbico y sacarosa en la obtenci贸n de plantas in vitro de sorghum bicolor para la formaci贸n de callos
T铆tulo en ingles: Effect of two cytokinin, ascorbic acid and sucrose to obtain in vitro shoots of sorghum for calli formation Resumen: El trabajo se realiz贸 con el objetivo de obtener brotes in vitro v铆a organog茅nesis directa en sorgo variedad CIAP 2E-95, para la formaci贸n de callos con estructuras embriog茅nicas. Se tomaron semillas maduras de plantas que crec铆an en condiciones controladas, las que se desinfectaron y fueron colocadas a germinaci贸n en un medio de cultivo con las sales MS, mioinositol 100 mg L-1, sacarosa 3%, pH 5,7, Fitagel 2,5 g L-1. Para la multiplicaci贸n de los brotes, se estudiaron diferentes concentraciones de 6 Bencilaminopurina (6-BAP) y kinetina. El empleo en el medio de cultivo de multiplicaci贸n con las sales MS y 6- BAP 0,22 mg L-1, increment贸 el n煤mero de brotes por explante y se observ贸 la presencia de oxidaci贸n fen贸lica. La oxidaci贸n fen贸lica se contrarrest贸 con la adici贸n al medio de cultivo de 50 mg L-1 de 谩cido asc贸rbico y se increment贸 significativamente el n煤mero de brotes, altura y n煤mero de hojas. En la obtenci贸n de brotes in vitro de calidad para la formaci贸n de callos, estos fueron engrosados con las sales MS, sin reguladores de crecimiento, 谩cido asc贸rbico 50 mg L-1, Fitagel 2,5 g L-1, pH 5,7 y 40 g L-1 de sacarosa. La formaci贸n de callos con estructuras embriog茅nicas a partir del cilindro central de las hojas enrolladas del segmento m谩s pr贸ximo a la base de los brotes engrosados se alcanz贸 a los 45 d铆as de cultivo. Los segmentos del cilindro central de las hojas enrolladas constituyen una fuente segura y promisoria de explantes para la formaci贸n de callos embriog茅nicos. Palabras clave: 6 BAP; brote in vitro; callos con estrucutras embriog茅nicos; kinetina. Abstract: The current work aimed to obtain in vitro shoots by direct organogenesis from sorghum variety CIAP 2E-95, for the formation of calluses with embryogenic structures. Mature seeds were collected from plants grown under controlled conditions, which were disinfected and were placed in a germination medium with MS salts, myo-inositol 100 mg L-1, 3% sucrose, pH 5.7, phytagel 2.5 g L-1. Different concentrations of 6-benzylaminopurine (6-BAP) and kinetin were studied for shoot multiplication. Using 6 - BAP 0.22 mg L-1 in the multiplication culture medium increased the number of shoots per explant, showing phenolic oxidation. Adding 50 mg L-1 of ascorbic acid to the culture medium resisted phenolic oxidation and the number of sprouts (5.0), height and number of leaves increased significantly. Quality in vitro buds for callus formation were obtained 聽stimulating the thickening by using MS salts, without growth regulators, ascorbic acid 50 mg L-1, phytagel 2.5 g L-1 and pH 5.7 and 40 g L-1 of sucrose. The largest diameter of shoots, height, number of leaves and roots of in vitro plants, was achieved after 21 days of cultured in MS salts with 40 g L-1 sucrose. Calluses formation with embryogenic structures from the central cylinder of curled leaves of the nearest segment to the base of thickened shoots was achieved after 45 days of culture. Segments from the central cylinder of the curled leaves are a safe source for the formation of embryogenic calli. Key words: 6-BAP; in vitro shoots; callus with embryogenic structures; kineti
Efecto de dos citoquininas, 谩cido asc贸rbico y sacarosa en la obtenci贸n de plantas in vitro de Sorghum bicolor para la formaci贸n de callos
The aim of the current work was to obtain in vitro shoots by direct organogenesis from sorghum variety CIAP 2E-95, for the formation of calluses with embryogenic structures. Mature seeds were collected from plants grown under controlled conditions, which were disinfected and were placed in a germination medium with MS salts, myo-inositol 100 mg L-1, 3% sucrose, pH 5.7, phytagel 2.5 g L-1. Different concentrations of 6-benzylaminopurine (6-BAP) and kinetin were studied for shoot multiplication. Using 6 - BAP 0.22 mg L-1 in the multiplication culture medium increased the number of shoots per explant, showing phenolic oxidation. Adding 50 mg L-1 of ascorbic acid to the culture medium resisted phenolic oxidation and the number of sprouts (5.0), height and number of leaves increased significantly. Quality in vitro buds for callus formation were obtained stimulating the thickening by using MS salts, without growth regulators, ascorbic acid 50 mg L-1, phytagel 2.5 g L-1 and pH 5.7 and 40 g L-1 of sucrose. The largest diameter of shoots, height, number of leaves and roots of in vitro plants, was achieved after 21 days of cultured in MS salts with 40 g L-1 sucrose. Calluses formation with embryogenic structures from the central cylinder of curled leaves of the nearest segment to the base of thickened shoots was achieved after 45 days of culture. Segments from the central cylinder of the curled leaves are a safe source for the formation of embryogenic callus.El trabajo se realiz贸 con el objetivo de obtener brotes in vitro v铆a organog茅nesis directa en sorgo variedad CIAP 2E-95, para la formaci贸n de callos con estructuras embriog茅nicas. Se tomaron semillas maduras de plantas que crec铆an en condiciones controladas, las que se desinfectaron y fueron colocadas a germinaci贸n en un medio de cultivo con las sales MS, mioinositol 100 mg L-1, sacarosa 3%, pH 5,7, Fitagel 2,5 g L-1. Para la multiplicaci贸n de los brotes, se estudiaron diferentes concentraciones de 6 Bencilaminopurina (6-BAP) y kinetina. El empleo en el medio de cultivo de multiplicaci贸n con las sales MS y 6- BAP 0,22 mg L-1, increment贸 el n煤mero de brotes por explante y se observ贸 la presencia de oxidaci贸n fen贸lica. La oxidaci贸n fen贸lica se contrarrest贸 con la adici贸n al medio de cultivo de 50 mg L-1 de 谩cido asc贸rbico y se increment贸 significativamente el n煤mero de brotes, altura y n煤mero de hojas. En la obtenci贸n de brotes in vitro de calidad para la formaci贸n de callos, estos fueron engrosados con las sales MS, sin reguladores de crecimiento, 谩cido asc贸rbico 50 mg L-1, Fitagel 2,5 g L-1, pH 5,7 y 40 g L-1 de sacarosa. La formaci贸n de callos con estructuras embriog茅nicas a partir del cilindro central de las hojas enrolladas del segmento m谩s pr贸ximo a la base de los brotes engrosados se alcanz贸 a los 45 d铆as de cultivo. Los segmentos del cilindro central de las hojas enrolladas constituyen una fuente segura y promisoria de explantes para la formaci贸n de callos embriog茅nicos