Сорбция ионов меди из азотнокислого раствора электролитического рафинирования серебра на хелатирующую смолу XUS 43605

The article is devoted to the sorption of copper ions from a silver electrolyte solution onto a chelating resin with hydroxypropylpicalylamine functional groups. The ion exchange capacity was determined under batch static and dynamic conditions, the effect of flow rate on the ion exchange capacity was shown, the possibility of desorption of copper ions by 1M and 2M sulfuric acid was investigated, the coefficient of separation of copper and silver under static and dynamic conditions is determinedВ статье исследуется сорбция ионов меди из раствора серебряного электролита на хелатный сорбент с гидроксипропилпикалиламиновыми функциональными группами. Определена рабочая емкость сорбента в статических и динамических условиях, показано влияние скорости подачи рабочего раствора в колонку на сорбцию ионов меди, исследована возможность десорбции меди 1М и 2М серной кислотой, определен коэффициент разделения меди и серебра в статических и динамических условия

Assigning new GO annotations to protein data bank sequences by combining structure and sequence homology

ABSTRACT Accompanying the discovery of an increasing number of proteins, there is the need to provide functional annotation that is both highly accurate and consistent. The Gene Ontology ™ (GO) provides consistent annotation in a computer readable and usable form; hence, GO annotation (GOA) has been assigned to a large number of protein sequences based on direct experimental evidence and through inference determined by sequence homology. Here we show that this annotation can be extended and corrected for cases where protein structures are available. Specifically, using the Combinatorial Extension (CE) algorithm for structure comparison, we extend the protein annotation currently provided by GOA at the European Bioinformatics Institute (EBI) to further describe the contents of the Protein Data Bank (PDB). Specific cases of biologically interesting annotations derived by this method are given. Given that the relationship between sequence, structure, and function is complicated, we explore the impact of this relationship on assigning GOA. The effect of superfolds (folds with many functions) is considered and, by compariso

The Use of Porous Silicate Materials with Different Structures for Acetalization of Furfural and 5-Hydroxymethylfurfural by Methanol

Изучено селективное ацеталирование фурфурола и 5-гидроксиметилфурфурола метанолом в присутствии гетерогенных катализаторов на основе диоксида кремния. Произведено сопоставление каталитической активности силикатных материалов единой химической природы (MCM-41, SBA-15 и силикагель) в реакциях ацеталирования фурфурола и 5-гидроксиметилфурфурола метанолом со структурными и текстурными свойствами катализатора. Мезоструктурированные силикатные материалы (MCM-41, SBA-15) позволяют получить метилацетали фурфурола и 5-гидроксиметилфурфурола с выходами 80–95 % от теоретического с селективностью до 98 %Selective acetalization of furfural and 5-hydroxymethylfurfural by methanol in the presence of silica catalysts was investigated. Catalytic activity of silica materials (MCM-41, SBA-15, and silicagel) in reaction of furfural and 5-hydroxymethylfurfural acetalization by methanol was brought into correlation with its structure and textural properties. Mesostructured silica materials (MCM-41, SBA- 15) allow obtaining of furfural and 5-hydroxymethylfurfural methylacetals with the 80-95 % yield and up to 98 % selectivit

Сорбция ионов меди из азотнокислого раствора электролитического рафинирования серебра на хелатирующую смолу Axionit BPA

The article considers a sorption method for extracting copper ions from a model nitric acid solution based on a solution of electrolytic refining of silver with using sorbents with bis-picolylamine functional groups: Dowex M-4195 and AXIONIT BPA. The possibility of extracting copper to residual concentrations in the solution of less than 10 mg/l is shown. The working capacity of both samples under batch conditions, as well as the dynamic exchange capacity and the full dynamic exchange capacity under dynamic сonditions (column test), are determined. The effect of the flow rate of the solution into the column on the kinetics of the sorption of copper ions on AXIONIT BPA is shown. The possibility of desorption of copper with a solution of 3 %, 5 % NH4OH was investigated, and a two-stage scheme of desorption of copper was tested: 5 % and 10 % with a solution of ammonium hydroxideВ статье рассмотрен сорбционный способ извлечения ионов меди из модельного азотнокислого раствора на основе электролитического рафинирования серебра на сорбенты c бис-пиколиламиновыми функциональными группами: Dowex M‑4195 и AXIONIT BPA. Показана возможность извлечения меди до остаточных концентраций в растворе менее 10 мг/л. Определена рабочая емкость обоих образцов в статических условиях, а также динамическая обменная емкость и полная динамическая обменная емкость в динамических условиях. Показано влияние скорости подачи раствора в колонку на кинетику сорбции ионов меди на AXIONIT BPA. Исследована возможность десорбции меди раствором 3 %, 5 % NH4OH, а также опробована двухстадийная схема десорбции меди: 5 % и 10 % раствором гидроксида аммони

Exploring Dynamic Metabolome of the HepG2 Cell Line: Rise and Fall

Both biological and technical variations can discredit the reliability of obtained data in omics studies. In this technical note, we investigated the effect of prolonged cultivation of the HepG2 hepatoma cell line on its metabolomic profile. Using the GC × GC-MS approach, we determined the degree of metabolic variability across HepG2 cells cultured in uniform conditions for 0, 5, 10, 15, and 20 days. Post-processing of obtained data revealed substantial changes in relative abundances of 110 metabolites among HepG2 samples under investigation. Our findings have implications for interpreting metabolomic results obtained from immortal cells, especially in longitudinal studies. There are still plenty of unanswered questions regarding metabolomics variability and many potential areas for future targeted and panoramic research. However, we suggest that the metabolome of cell lines is unstable and may undergo significant transformation over time, even if the culture conditions remain the same. Considering metabolomics variability on a relatively long-term basis, careful experimentation with particular attention to control samples is required to ensure reproducibility and relevance of the research results when testing both fundamentally and practically significant hypotheses

The Use of Porous Silicate Materials with Different Structures for Acetalization of Furfural and 5-Hydroxymethylfurfural by Methanol

Изучено селективное ацеталирование фурфурола и 5-гидроксиметилфурфурола метанолом в присутствии гетерогенных катализаторов на основе диоксида кремния. Произведено сопоставление каталитической активности силикатных материалов единой химической природы (MCM-41, SBA-15 и силикагель) в реакциях ацеталирования фурфурола и 5-гидроксиметилфурфурола метанолом со структурными и текстурными свойствами катализатора. Мезоструктурированные силикатные материалы (MCM-41, SBA-15) позволяют получить метилацетали фурфурола и 5-гидроксиметилфурфурола с выходами 80–95 % от теоретического с селективностью до 98 %Selective acetalization of furfural and 5-hydroxymethylfurfural by methanol in the presence of silica catalysts was investigated. Catalytic activity of silica materials (MCM-41, SBA-15, and silicagel) in reaction of furfural and 5-hydroxymethylfurfural acetalization by methanol was brought into correlation with its structure and textural properties. Mesostructured silica materials (MCM-41, SBA- 15) allow obtaining of furfural and 5-hydroxymethylfurfural methylacetals with the 80-95 % yield and up to 98 % selectivit

Exploiting Multi-Omics Profiling and Systems Biology to Investigate Functions of TOMM34

Although modern biology is now in the post-genomic era with vastly increased access to high-quality data, the set of human genes with a known function remains far from complete. This is especially true for hundreds of mitochondria-associated genes, which are under-characterized and lack clear functional annotation. However, with the advent of multi-omics profiling methods coupled with systems biology algorithms, the cellular role of many such genes can be elucidated. Here, we report genes and pathways associated with TOMM34, Translocase of Outer Mitochondrial Membrane, which plays role in the mitochondrial protein import as a part of cytosolic complex together with Hsp70/Hsp90 and is upregulated in various cancers. We identified genes, proteins, and metabolites altered in TOMM34-/- HepG2 cells. To our knowledge, this is the first attempt to study the functional capacity of TOMM34 using a multi-omics strategy. We demonstrate that TOMM34 affects various processes including oxidative phosphorylation, citric acid cycle, metabolism of purine, and several amino acids. Besides the analysis of already known pathways, we utilized de novo network enrichment algorithm to extract novel perturbed subnetworks, thus obtaining evidence that TOMM34 potentially plays role in several other cellular processes, including NOTCH-, MAPK-, and STAT3-signaling. Collectively, our findings provide new insights into TOMM34’s cellular functions