118 research outputs found

    Cryogenic Safety - HSE seminar

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    To avoid mistakes during the calculation, CEA/SBT has decided to write a software that take into account all the situations it is possible to encountered (subcritical state, supercritical state, …). The goal is to permit to the engineer in charge of a cryostat manufacturing to perform this calculation; he is the only person able to do the accidental analysis which is fundamental for the sizing of the safety device. The software performed will be presented

    Les Beurres d’Isigny

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    Longtemps limitée, la consommation de beurre augmente fortement sous l'Ancien Régime. Des soupers fins aux réfectoires des abbayes, le beurre s'affirme dans l'alimentation de la moitié nord du royaume de France. Répondant à cette demande, une partie du Cotentin et du Bessin se tourne alors vers l'approvisionnement des villes. Au XVIIIe siècle, le beurre frais d'Isigny est déjà réputé à Paris. La reconstitution des circuits d'approvisionnement confirme la sensibilité des campagnes normandes aux rythmes de consommation des clientèles urbaines et au jeu de la concurrence. Elle met en lumière le rôle des marchands de beurre, intermédiaires économiques et culturels. En amont, une agriculture originale se développe. L'herbe prend le pas sur les labours dans un bocage en cours de constitution, tandis que les marais se transforment. À travers l'histoire des premiers siècles d'une denrée alimentaire de renom, cet ouvrage se veut une réflexion sur la spécialisation agricole en Normandie avant le XIXe siècle

    Resin straw as an alternative system to securely store frozen microorganisms.

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    International audienceFreezing of prokaryotic and eukaryotic microorganisms is the main interest in the study of cold stress responses of living organisms. In parallel, applications which arise from this approach are of two types: (i) optimization of the frozen starters used in food processing; and (ii) improvement of the ex situ preservation of microorganisms in collections. Currently, cryopreservation of microorganisms in collections is carried out in cryotubes, and bibliographical references related to freezing microorganisms packaged in straws are scarce. In this context, a preliminary study was completed to evaluate the technological potential of ionomeric resin straws compared to polycarbonate cryo-tubes. Survival under freezing stress was tested on three microorganisms selected for their biotechnological interest: two lactic acid bacteria, Lactococcus lactis subsp. cremoris and Lactobacillus delbrueckii subsp. bulgaricus and a deuteromycete fungus, Geotrichum candidum. The stress was carried out by repeated freezing-thawing cycles to artificially accelerate the lethal effect of freezing on the microorganisms. Two main results were obtained: (i) the survival rate values (per freezing-thawing cycle) seems to depend on the thermal type of the studied microorganism, and (ii) there was no, under our experimental conditions, significant difference between straws and tubes. However, conservation in the resin straws lead to a slight increase in the survival of L. cremoris and G. candidum compared to microtubes. In those conditions, straws seems an alternative system to securely store frozen microorganisms with three main characteristics: (i) a high resistance to thermal stress, (ii) a safe closing by hermetic weld, and (iii) a system for inviolable identification

    Politiques d'innovation, espace régional et dynamique des territoires : un essai de caractérisation dans le contexte français

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    French system of innovation, lever effects, regions, regional innovation policies, territorial innovation system
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