Fitness correlates of blubber oxidative stress and cellular defences in grey seals (<i>Halichoerus grypus</i>):support for the life-history-oxidative stress theory from an animal model of simultaneous lactation and fasting

Life-history-oxidative stress theory predicts that elevated energy costs during reproduction reduce allocation to defences and increase cellular stress, with fitness consequences, particularly when resources are limited. As capital breeders, grey seals are a natural system in which to test this theory. We investigated oxidative damage (malondialdehyde (MDA) concentration) and cellular defences (relative mRNA abundance of heat shock proteins (Hsps) and redox enzymes (REs)) in blubber of wild female grey seals during the lactation fast (n = 17) and summer foraging (n = 13). Transcript abundance of Hsc70 increased, and Nox4, a pro-oxidant enzyme, decreased throughout lactation. Foraging females had higher mRNA abundance of some Hsps and lower RE transcript abundance and MDA concentrations, suggesting they experienced lower oxidative stress than lactating mothers, which diverted resources into pup rearing at the expense of blubber tissue damage. Lactation duration and maternal mass loss rate were both positively related to pup weaning mass. Pups whose mothers had higher blubber glutathione-S-transferase (GST) expression at early lactation gained mass more slowly. Higher glutathione peroxidase (GPx) and lower catalase (CAT) were associated with longer lactation but reduced maternal transfer efficiency and lower pup weaning mass. Cellular stress, and the ability to mount effective cellular defences, could proscribe lactation strategy in grey seal mothers and thus affect pup survival probability. These data support the life-history-oxidative stress hypothesis in a capital breeding mammal and suggest lactation is a period of heightened vulnerability to environmental factors that exacerbate cellular stress. Fitness consequences of stress may thus be accentuated during periods of rapid environmental change

Modelling the regional sensitivity of snowmelt, soil moisture, and streamflow generation to climate over the Canadian Prairies using a basin classification approach

This study evaluated the effects of climate perturbations on snowmelt, soil moisture, and streamflow generation in small Canadian Prairies basins using a modelling approach based on classification of basin biophysical characteristics. Seven basin classes that encompass the entirety of the Prairies Ecozone in Canada were determined by cluster analysis of these characteristics. Individual semi-distributed virtual basin (VB) models representing these classes were parameterized in the Cold Regions Hydrological Model (CRHM) platform, which includes modules for snowmelt and sublimation, soil freezing and thawing, actual evapotranspiration (ET), soil moisture dynamics, groundwater recharge, and depressional storage dynamics including fill and spill runoff generation and variable connected areas. Precipitation (P) and temperature (T) perturbation scenarios covering the range of climate model predictions for the 21st century were used to evaluate climate sensitivity of hydrological processes in individual land cover and basin types across the Prairies Ecozone. Results indicated that snow accumulation in wetlands had a greater sensitivity to P and T than that in croplands and grasslands in all basin types. Wetland soil moisture was also more sensitive to T than the cropland and grassland soil moisture. Jointly influenced by land cover distribution and local climate, basin-average snow accumulation was more sensitive to T in the drier and grassland-characterized basins than in the wetter basins dominated by cropland, whilst basin-average soil moisture was most sensitive to T and P perturbations in basins typified by pothole depressions and broad river valleys. Annual streamflow had the greatest sensitivities to T and P in the dry and poorly connected Interior Grasslands (See Fig. 1) basins but the smallest in the wet and well-connected Southern Manitoba basins. The ability of P to compensate for warming-induced reductions in snow accumulation and streamflow was much higher in the wetter and cropland-dominated basins than in the drier and grassland-characterized basins, whilst decreases in cropland soil moisture induced by the maximum expected warming of 6 ∘C could be fully offset by a P increase of 11 % in all basins. These results can be used to (1) identify locations which had the largest hydrological sensitivities to changing climate and (2) diagnose underlying processes responsible for hydrological responses to expected climate change. Variations of hydrological sensitivity in land cover and basin types suggest that different water management and adaptation methods are needed to address enhanced water stress due to expected climate change in different regions of the Prairies Ecozone.</p

Effects of needleleaf forest cover on radiation and snowmelt dynamics in the Canadian Rocky Mountains

Abstract: Radiation is the main energy source for snowpack warming and melt in mountain needleleaf forests, and runoff from these forests is the main contributor to spring river flows in western North America. Utilizing extensive field obser-vations, the effect of needleleaf forest cover on radiation and snowmelt timing was quantified at pine and spruce forest sites and nearby clearings of varying slope and aspect in an eastern Canadian Rocky Mountain headwater basin. Compared with open clearing sites, shortwave radiation was much reduced under forest cover, resulting in smaller differences in melt timing between forested slopes relative to open slopes with different aspects. In contrast, longwave radiation to snow was substantially enhanced under forest cover, especially at the dense spruce forest sites where longwave radiation dominated total energy for snowmelt. In both pine and spruce environments, forest cover acted to substantially reduce total radiation to snow and delay snowmelt timing on south-facing slopes while increasing total radiation and advancing snowmelt timing on north-facing slopes. Results strongly suggest that impacts on radiation to snow and snowmelt timing from changes in mountain forest cover will depend much on the slope and aspect at which changes occur. Résume ́ : Le rayonnement est la principale source d’énergie qui réchauffe et fait fondre la couche de neige dans les forêts alpines de conifères ou ̀ le ruissellement contribue dans une large mesure au débit printanier des rivières dans l’ouest de l’Amérique du Nord. A ` l’aide de nombreuses observations sur le terrain, dans un bassin de tête de la partie est des monta-gnes Rocheuses canadiennes, l’effet du couvert de forêt de conifères sur le rayonnement et sur le moment de la fonte d

EUV spectra of highly-charged ions W54+^{54+}-W63+^{63+} relevant to ITER diagnostics

We report the first measurements and detailed analysis of extreme ultraviolet (EUV) spectra (4 nm to 20 nm) of highly-charged tungsten ions W$^{54+}$ to W$^{63+}$ obtained with an electron beam ion trap (EBIT). Collisional-radiative modelling is used to identify strong electric-dipole and magnetic-dipole transitions in all ionization stages. These lines can be used for impurity transport studies and temperature diagnostics in fusion reactors, such as ITER. Identifications of prominent lines from several W ions were confirmed by measurement of isoelectronic EUV spectra of Hf, Ta, and Au. We also discuss the importance of charge exchange recombination for correct description of ionization balance in the EBIT plasma.Comment: 11 pages, 4 figure

Neutron cross sections for He-3 at epithermal energies

High accuracy, absolute measurements of the neutron total cross section for He-3 are reported for incident neutron energies 0.1-400 eV. The measurements were performed at the LANSCE short-pulse neutron spallation source. Using the previously determined cross section for neutron elastic scattering, 3.367+/-0.019 b, we extract a new value for the energy dependence of the He-3(n,p)He-3 reaction cross section, sigma(np)=(849.77+/-0.14+/-1.02)E-1/2-(1.253+/-0.00+/-(+0.008)(-0.049))b, where the neutron energy is expressed in eV. The first uncertainty is statistical, the second systematic

Can auditors be independent? – Experimental evidence on the effects of client type

Recent regulatory initiatives stress that an independent oversight board, rather than the management board, should be the client of the auditor. In an experiment, we test whether the type of client affects auditors’ independence. Unique features of the German institutional setting enable us to realistically vary the type of auditors’ client as our treatment variable: we portray the client either as the management preferring aggressive accounting or the oversight board preferring conservative accounting. We measure auditors’ perceived client retention incentives and accountability pressure in a post-experiment questionnaire to capture potential threats to independence. We find that the type of auditors’ client affects auditors’ behaviour contingent on the degree of the perceived threats to independence. Our findings imply that both client retention incentives and accountability pressure represent distinctive threats to auditors’ independence and that the effectiveness of an oversight board in enhancing auditors’ independence depends on the underlying threat