Development of an in-house ELISA to detect anti-HPV16-L1 antibodies in serum and dried blood spots

Measuring anti-HPV antibody levels is important for surveillance of the immunological response to both natural infection and vaccination. Here, an ELISA test for measurement of HPV-16L1 antibodies was developed and validated in sera and dried blood spots. An in-house ELISA was developed for measuring anti-HPV-16L1 IgA and IgG levels. The assay was standardized against WHO international standard serum and validated on serum, dried blood spots and cervical liquid based cytology samples from women attending colposcopy clinics in Scotland. Antibody avidity index was also measured in serum samples. The average HPV 16-L1 specific IgG and IgA levels measured in sera, in women attending a routine colposcopy service were 7.3 units/ml and 8.1 units/ml respectively. Significant correlations between serum and dried blood spot eluates for both IgG and IgA were observed indicating that the latter serve as a credible proxy for antibody levels. Average IgG Avidity Index was 35% (95% CI 25%-45%) suggesting previous, historical challenge with natural infection. This ELISA has potential for use in epidemiological and field studies of antibody prevalence and if coupled with avidity measurement may be of use in individual case monitoring of vaccine responses and failures

A confirmation of agreement of different approaches for scalar gauge-invariant metric perturbations during inflation

We revisit an extension of the well-known formalism for gauge-invariant scalar metric fluctuations, to study the spectrums for both, the inflaton and gauge invariant (scalar) metric fluctuations in the framework of a single field inflationary model where the quasi-exponential expansion is driven by an inflation which is minimally coupled to gravity. The proposal here examined is valid also for fluctuations with large amplitude, but for cosmological scales, where vector and tensor perturbations can be neglected and the fluid is irrotacional.Comment: Version accepted in EPJC with new title. 11 pages, no figure