Verification of the Isolation of the Chalcone Isomerase Gene from Glycine Max

In this paper we report the verified isolation of the gene encoding the flavonoid-biosynthetic enzyme chalcone flavonone isomerase (CHI) from Glycine max as well as the procedures used to generate the results. We began by electroeluting an 865 base pair (bp) Hindlll fragment from pCHIl. We then restricted the pDRIO plasmid with Hindlll. In order to test for homology, we hybridized the pDRIO fragment with nick translated pCHIl fragment as a probe. For a second electrophoresis experiment, we cut pDRIO with EcoRI and Hindlll. We also restricted several different pDRIO plasmids with a variety of deletions. After performing a Southern blot on the second restriction, we were able to determine that the homology for the CHI gene lies in a 1983 bp segment between the multicloning Hindlll site and Pstl site of the insert

Attitudes of Black Socioeconomic Classes In The Greater Kansas City Area Relative To Black Psychology

Four hundred and twenty-nine questionnaires developed by the investigator were distributed to Black white collar, blue collar, farm, and service workers in the Metropolitan Kansas City Area. The questionnaires, of which 383 were returned, were designed to determine whether the subjects perceived themselves as being different from the dominant society, to assess their attitudes about the need for a Black Psychology which concerns itself with the unique behavioral characteristics of Black Americans, and to see whether those assessments differed according to socioeconomic status. The subjects who comprised the convenience sample were contacted through several agencies including the U.S. Department of Labor, nursing homes, hospitals, construction companies, and business unions. Findings were: 1.) Blacks perceived themselves as equal to other people, yet acknowledged the presence of measurable differences in the Black social structure and family life; 2.) the degree of perceived differences in heritage, need for psychology, priorities, etc. varied according to socioeconomic status; and 3.) the need for a Black Psychology or a change in the prevailing psychological framework was overdue. All results were significant at the .01 or .001 levels

Summary of Action Taken Following Delegation Trip to Washington, D.C on Behalf of the Three Affiliated Tribes, May 9 (no year given)

This news release, dated May 9 (no year given), provides a summary of what was accomplished by a group of delegates from the Three Affiliated Tribes in Washington D.C. to attend hearings before the United States (US) House Interior Committee. Delegates included Martin Cross, William J. Deane, and O. R. Shulte. The list of accomplishments includes a bill to provide for the distribution of funds held in trust to be distributed by on a per capita basis, an amendment to a bill that authorizes members of the Three Affiliated tribes to acquire trust in tribal lands, and a bill to provide for fishing and grazing rights on land taken for the Garrison Dam project. The summary provides further commentary on these issues.https://commons.und.edu/burdick-papers/1038/thumbnail.jp

Sputtering yield measurements at glancing incidence using a quartz crystal microbalance

Low energy sputtering yields at grazing incidence have been investigated experimentally using a quartz crystal microbalance (QCM) technique. This method involved precoating the QCM with a thin film of the desired target material and relating the resonance frequency shift directly to mass loss during ion bombardment. A highly focused, low divergence ion beam provided a well defined incidence angle. Focusing most of the ion current on the center of the target allowed for higher sensitivity by taking into account the radial mass sensitivity of the QCM. Measurements of Mo, Cu, and W sputtering yields were taken for low energy (80–1000 eV) Xe+ and Ar+ to validate this experimental method. The target films ranged from 3.5 to 8.0 µm in thickness and were deposited so that their crystal structure and density would match those of the bulk material as closely as possible. These properties were characterized using a combination of scanning electron microscope imagery, profilometry, and x-ray diffraction. At normal incidence, the sputtering yields demonstrated satisfactory agreement with previously published work. At angles of incidence up to 40° off normal, the data agreed well with predictions from existing theoretical models. Sputtering yields were found to increase by a factor of 1.6 over this range. The optimum angle for sputtering occurred at 55°, after which the yields rapidly decreased. Measurements were taken up to 80° from the surface normal

Comparing Neanderthal and Modern Human Long Bone Loading History from Cross-Sectional Geometry

We evaluate here efforts to compare archaic and modern human limb loading from long bone cross sectional. Recent studies find that cross sectional properties (I, J, Z) calculated from second moments of area (SMA) are similar in Neanderthals and early modern humans when adjusted for body mass and limb length, but differ in cross-sectional shape (e.g., Ix/Iy). These results suggest the two taxa had similar magnitudes but different patterns of locomotor loading. Such interpretations, however, assume that long bones are deformed like long, straight beams in pure bening, with neutral axes (NA) that run through the cross-sectional area centroids. We test this assumption experimentally using exercised sheep with rosette strain gauges mounted at three locations around the midshaft of the tibia and metatarsal. Calculation of normal strain distributions at the midshaft indicate that the NA does not run through the area centroid, largely because of the combined effects of bending and compression. In addition, orientation of the centroidal axes around which maximum SMAs (Imax) are calculated are unrelated to the planes in which the bines bend. Because SMAs are fourth-power functions, cross-sectional properties that assume the NA runs through the area centroid yield substantial errors in magnitude (up to 100%) compared to cross-sectional properties calculated around experimentally-determined NAs. The polar moment of area, J, is least subject to error. Applying these analyses to the hominoid fossil record indicates that SMAs neither support nor refute the hypothesis that Neanderthals and early modern humans had different magnitudes or patterns of loading.AnthropologyHuman Evolutionary Biolog

Atrial Arrhythmias in Astronauts - Summary of a NASA Summit

Background and Problem Definition: To evaluate NASA s current standards and practices related to atrial arrhythmias in astronauts, Space Medicine s Advanced Projects Section at the Johnson Space Center was tasked with organizing a summit to discuss the approach to atrial arrhythmias in the astronaut cohort. Since 1959, 11 cases of atrial fibrillation, atrial flutter, or supraventricular tachycardia have been recorded among active corps crewmembers. Most of the cases were paroxysmal, although a few were sustained. While most of the affected crewmembers were asymptomatic, those slated for long-duration space flight underwent radiofrequency ablation treatment to prevent further episodes of the arrhythmia. The summit was convened to solicit expert opinion on screening, diagnosis, and treatment options, to identify gaps in knowledge, and to propose relevant research initiatives. Summit Meeting Objectives: The Atrial Arrhythmia Summit brought together a panel of six cardiologists, including nationally and internationally renowned leaders in cardiac electrophysiology, exercise physiology, and space flight cardiovascular physiology. The primary objectives of the summit discussions were to evaluate cases of atrial arrhythmia in the astronaut population, to understand the factors that may predispose an individual to this condition, to understand NASA s current capabilities for screening, diagnosis, and treatment, to discuss the risks associated with treatment of crewmembers assigned to long-duration missions or extravehicular activities, and to discuss recommendations for prevention or management of future cases. Summary of Recommendations: The summit panel s recommendations were grouped into seven categories: Epidemiology, Screening, Standards and Selection, Treatment of Atrial Fibrillation Manifesting Preflight, Atrial Fibrillation during Flight, Prevention of Atrial Fibrillation, and Future Researc