Acaricidal activity of ethanolic extract from aerial parts of Tagetes patula L. (Asteraceae) against larvae and engorged adult females of Rhipicephalus sanguineus (Latreille, 1806)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Background: The tick Rhipicephalus sanguineus is the species with the largest worldwide distribution and is proven to be involved in the transmission of pathogens such as Babesia canis, Ehrlichia canis, Coxiella burnetii, Rickettsia ricketsii, Rickettsia conorii, among others. Studies have demonstrated acquisition of resistance to some of the active principles used in commercial formulations of acaricides. Tagetes patula (Asteraceae) is a plant with highlighted economic and commercial importance due to the production of secondary metabolites with insecticide and acaricide potential, mainly flavonoids, thiophenes and terpenes. Methods: The in vitro acaricide action of the ethanolic 70% extract from aerial parts of T. patula, obtained by percolation, was evaluated against larvae and engorged adult females of Rhipicephalus sanguineus by immersion test for 5 minutes. The chemical characterization of this extract was done by liquid chromatography coupled with mass spectrometry (LC-MS), using direct injection of sample. Results: Despite T. patula not proving lethal to adults in any of the concentrations tested, at 50.0 mg/mL oviposition rate decreased by 21.5% and eliminated 99.78% of the larvae. Also it was determined that the best results were obtained with 5 minutes of immersion. From the chromatographic analysis twelve O-glycosylated flavonoids were identified. Conclusions: This is the first report on the acaricidal activity of T. patula extract against Rh. sanguineus. If we consider the application of the product in the environment, we could completely eliminate the larval stage of development of the ixodid Rh. sanguineus.5Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Infectiousness of Sylvatic and Synanthropic Small Rodents Implicates a Multi-host Reservoir of Leishmania (Viannia) braziliensis

Submitted by Adagilson Silva ([email protected]) on 2017-09-15T18:49:59Z No. of bitstreams: 1 26448187 2015 and-inf.oa.pdf: 409773 bytes, checksum: 86f9fa8878143d50fff9aaa5d5cfab39 (MD5)Approved for entry into archive by Adagilson Silva ([email protected]) on 2017-09-15T19:47:18Z (GMT) No. of bitstreams: 1 26448187 2015 and-inf.oa.pdf: 409773 bytes, checksum: 86f9fa8878143d50fff9aaa5d5cfab39 (MD5)Made available in DSpace on 2017-09-15T19:47:18Z (GMT). No. of bitstreams: 1 26448187 2015 and-inf.oa.pdf: 409773 bytes, checksum: 86f9fa8878143d50fff9aaa5d5cfab39 (MD5) Previous issue date: 2015Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, BrasilThe possibility that a multi-host wildlife reservoir is responsible for maintaining transmission of Leishmania (Viannia) braziliensis causing human cutaneous and mucocutaneous leishmaniasis is tested by comparative analysis of infection progression and infectiousness to sandflies in rodent host species previously shown to have high natural infection prevalences in both sylvatic or/and peridomestic habitats in close proximity to humans in northeast Brazil