New records of mosquito species (Diptera: Culicidae) for Bahia (Brazil)

Federal University of Piauí State - Professora Cinobelina Elvas. Bom Jesus, PI, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Saint Louis Zoo. Institute for Conservation Medicine. Saint Louis, MO, USA.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.We provide seven new identified mosquitoes in the Bahia State, Brazil: Coquillettidia nigricans, Johnbelkinia longipes, Limatus pseudomethysticus, Psorophora albipes, Sabethes belisarioi, Sabethes cyaneus and Sabethes quasicyaneus. This new finding which expands the known distribution of these seven species of mosquitoes, is of great importance as we work for the development of preventive measures for arboviruses in Brazil and globally. In other regions of the world, the culicids we report are known vectors of important arboviruses of human and non-human animal concern, including yellow fever, Saint Louis encephalitis, equine encephalitis, Guama, Una, Mayaro, wyeomyia and Kairi viruses, and may play a role in the epidemiology of these diseases in Bahia as well. Our work also highlights the paucity of data on the insect diversity in different environments in Brazil

Effects of vaccination against the H1N1 virus on BDNF and TNF-? plasma levels in pregnant women.

Background: Vaccination is a widespread strategy to protect women and their children during fetal development. However, there is a lack of knowledge about potential effects of H1N1 vaccination on concentration of cytokines that are important to mother?s central nervous system functions and fetal neurodevelopment. Objective: The main purpose of the present study was to evaluate such interaction. The specific goals were to study the effects of vaccination against the H1N1 virus on plasma levels of the Brain Derived Neurotrophic Factor(BDNF), Tumor Necrosis Factor- (TNF-) and TNF- Receptors 1 and 2 (sTNFR1; sTNFR2), in different periods of gestation. Methods: Data were obtained during the period of 6 months in 2010, from a sample of 94 pregnant women who were using the health care service of Concei??o do Mato Dentro, a rural area in the state of Minas Gerais, Brazil. Seventeen women were in the first trimester of pregnancy, forty were in the second trimester and 37 were in the third trimester. Each of these groups was divided into two subgroups as follows: immunized against the H1N1 virus (I) and non-immunized (NI). Plasma concentrations of BDNF, TNF-, sTNFR1 and sTNFR2 were measured using the sandwich ELISA. Results: There was no difference in cytokine or neurotrophic factor levels evaluated between groups I and NI in any trimesters. Conclusion: These results show that the recommendation of vaccination against the H1N1 virus for all pregnant women as a public health measure could be considered safe, regarding aspects related to the role playe

Single nucleotide polymorphisms in <i>E. gingivalis</i> isolates.

<p>Single nucleotide polymorphisms in <i>E. gingivalis</i> isolates.</p

LSSP-PCR. Polyacrylamide gel (7.5%) stained with silver nitrate.

<p>Primers: A) EGO-1 and B) EGO-2. Lane 1: molecular marker 1 Kb (Invitrogen); lanes 2–25: HIV(+)/AIDS samples; lane 26: negative control.</p

Phylogenetic relationship of <i>Entamoeba</i> spp. from HIV infected patients.

<p>Neighbor-joining method was performed in 24 taxa at the SSU rRNA locus. The optimal tree with the sum of branch length = 1.32 is shown and the bootstrap values were added to phylogenetic branches.</p

Analysis of the genetic variability of the small ribosomal subunit in the 18S rRNA of <i>E. gingivalis</i> in clinical samples from HIV(+)/AIDS patients, detected by LSSP-PCR.

<p>M = male; F = female; CD4 =  CD4⁺ cell count; HIV− = HIV negative; UR = unrealized.</p

Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus

Work at UTMB was supported by NIH grant U54 AI057156, R24 AI120942 (HG, RBT, NV) and by NIH contract HHSN272202000040I/ HHSN200004/D04 (HG, RBT, NV). MAC was supported by Programa de Doctorados Nacionales – Colciencias” (Convocatoria 567) (MAC) PhD fellowship. The field collection in Colombia was supported in part by Colciencias C.T-695-2014 and by Colciencias grant 111549326198. This work was supported in part by CNPq (Brazilian National Council for Research and Development) grant no. 302032/2011-8 (MRTN). We also acknowledge support from the Yale Institute for Biospheric Studies Center for EcoEpidemiology and from the U.S. National Park Service (Collecting Permit #EVER-2013-SCI-0032)Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Universidad Nacional de Colombia. Facultad de Ciencias,Ciencias. Grupo de Investigación en Sistemática Molecular. Medellín, Medellín, Colombia / Universidad de Antioquia. Programa de Estudio y Control de Enfermedades Tropicales - PECET - SIU-Sede de Investigación Universitaria. Medellín, Colombia.University of Texas Medical Branch. Department of Pathology and Center for Biodefense and Emerging Infectious Diseases. Galveston, TX, United States / University of Texas Medical Branch. Center for Tropical Diseases. Galveston, TX, United States / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Vale S.A. Projeto Ferro Carajás. Canaã dos Carajás, PA, Brazil.Yale University. Department of Ecology and Evolutionary Biology. New Haven, CT, United States.University of Washington. School of Public Health. Seattle, WA, United States.Universidad Nacional de Colombia. Facultad de Ciencias,Ciencias. Grupo de Investigación en Sistemática Molecular. Medellín, Colombia.Universidad Nacional de Colombia. Facultad de Ciencias,Ciencias. Grupo de Investigación en Sistemática Molecular. Medellín, Medellín, Colombia / Universidad de Antioquia. Programa de Estudio y Control de Enfermedades Tropicales - PECET - SIU-Sede de Investigación Universitaria. Medellín, Colombia.Universidad Nacional de Colombia. Facultad de Ciencias,Ciencias. Grupo de Investigación en Sistemática Molecular. Medellín, Medellín, Colombia / Universidad de Antioquia. Programa de Estudio y Control de Enfermedades Tropicales - PECET - SIU-Sede de Investigación Universitaria. Medellín, Colombia.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Vale S.A. Projeto Ferro Carajás. Canaã dos Carajás, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of Texas Medical Branch. Department of Biochemistry and Molecular Biology. Galveston, TX, United States.University of Texas Medical Branch. Department of Biochemistry and Molecular Biology. Galveston, TX, United States.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States.University of Texas Medical Branch. Department of Pathology and Center for Biodefense and Emerging Infectious Diseases. Galveston, TX, United States / University of Texas Medical Branch. Center for Tropical Diseases. Galveston, TX, United States / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of Texas Medical Branch. Department of Pathology and Center for Biodefense and Emerging Infectious Diseases. Galveston, TX, United States / University of Texas Medical Branch. Center for Tropical Diseases. Galveston, TX, United States / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new negevirus isolates obtained in Brazil, Colombia, Peru, Panama, USA and Nepal. We demonstrated that these arthropod restricted viruses are clustered in two major phylogenetic groups with origins related to three plant virus genera (Cilevirus, Higrevirus and Blunevirus). Molecular analyses demonstrated that specific host correlations are not present with most negeviruses; instead, high genetic variability, wide host-range, and cross-species transmission were noted. The data presented here also revealed the existence of five novel insect-specific viruses falling into two arthropod-restrictive virus taxa, previously proposed as distinct genera, designated Nelorpivirus and Sandewavirus. Our results provide a better understanding of the molecular epidemiology, evolution, taxonomy and stability of this group of insect-restricted viruses