Macrophage Polarization in Health and Disease

Macrophages are terminally differentiated cells of the mononuclear phagocyte system that also encompasses dendritic cells, circulating blood monocytes, and committed myeloid progenitor cells in the bone marrow. Both macrophages and their monocytic precursors can change their functional state in response to microenvironmental cues exhibiting a marked heterogeneity. However, there are still uncertainties regarding distinct expression patterns of surface markers that clearly define macrophage subsets, particularly in the case of human macrophages. In addition to their tissue distribution, macrophages can be functionally polarized into M1 (proinflammatory) and M2 (alternatively activated) as well as regulatory cells in response to both exogenous infections and solid tumors as well as by systems biology approaches

P300-Based BCI Mouse With Genetically-Optimized Analogue Control

In this paper we propose a brain-computer interface (BCI) mouse based on P300 waves in electroencephalogram (EEG) signals. The system is analogue in that at no point a binary decision is made as to whether or not a P300 was actually produced in response to the stimuli. Instead, the 2-D motion of the pointer on the screen, using a novel BCI paradigm, is controlled by directly combining the amplitudes of the output produced by a filter in the presence of different stimuli. This filter and the features to be combined within it are optimised by an evolutionary algorithm. © 2006 IEEE

Risk Factors for Immediate and Delayed-Onset Fever After Percutaneous Transhepatic Biliary Drainage

Objectives To prospectively investigate the pre and intraprocedural risk factors for immediate (IF) and delayedonset (DOF) fever development after percutaneous transhepatic biliary drainage (PTBD). Methods Institutional review board approval and informed patient consent were obtained. Between February 2013 and February 2014, 97 afebrile patients (77 at the Sapienza University of Rome, Italy and 20 at the Sun Yatsen University of Guangzhou, China) with benign (n = 31) and malignant (n = 66) indications for a first PTBD were prospectively enrolled. Thirty pre- and intra-procedural clinical/radiological characteristics, including the amount of contrast media injected prior to PTBD placement, were collected in relation to the development of IF (within 24 h) or DOF (after 24 h). Fever was defined as C37.5 C. Binary logistic regression analysis was used to assess independent associations with IF and DOF. Results Fourteen (14.4 %) patients developed IF and 17 (17.5 %) developed DOF. At multivariable analysis, IF was associated with pre-procedural absence of intrahepatic bile duct dilatation (OR 63.359; 95 % CI 2.658–1510.055; P = 0.010) and low INR (OR 4.7 9 10-4 ; 95 % CI 0.000–0.376; P = 0.025), while DOF was associated with unsatisfactory biliary drainage at the end of PTBD (OR 4.571; 95 % CI 1.161–17.992; P = 0.030)

Renal papillary carcinoma developed in a kidney transplant recipient with late IgA-nephropathy

With improvements in immunosuppressive therapy, patient and graft survival in renal transplant recipients have been prolonged. Increasing donor age and patient survival rates have been related to an increase in the number of de novo tumors. Posttransplant malignancy in these patients is an important cause of graft loss and death in these patients. Among cancers occurring after a kidney transplant, renal cell carcinoma is the fifth most common malignancy after lymphoproliferative disorders, and skin, gastrointestinal, and lung cancers. When nonmelanoma skin cancers and in situ carcinoma of the cervix are excluded from malignancies, renal cell carcinoma accounts for 2% of all cancers in the general population, which increases to 5% in solid-organ recipients. The majority of renal cell carcinomas found in transplant recipients develop in the recipient 's native kidneys, but only 9% of tumors develop in the allograft itself. Tumors transmitted by donors represent only 0.02% to 0.2% of cases. Most de novo allograft renal cell carcinomas are single tumors. The mechanisms of development of renal cell carcinoma in renal grafts are not completely understood

Sensors Characterization for a Calibration-Free Connected Smart Insole for Healthy Ageing

The design of technological aids to assist older adults in their ageing process and to ensure proper attendance and care, despite the decreasing percentage of young people in the demographic profiles of many developed countries, requires the proper selection of sensing components, in order to come up with devices that can be easily used and integrated into everyday life. This paper addresses the metrological characterization of pressure sensors to be inserted into smart insoles aimed at monitoring the older adult’s physical activity levels. Two types of sensing elements are evaluated and a recommendation provided, based on the main requirement of designing a calibration-free insole: in this case, the pressure sensor should act as a switch, and the FSR 402 Short sensing element appears to be the proper solution to adopt

Online Change Point Detection in Molecular Dynamics With Optical Random Features

Proteins are made of atoms constantly fluctuating, but can occasionally undergo large-scale changes. Such transitions are of biological interest, linking the structure of a protein to its function with a cell. Atomic-level simulations, such as Molecular Dynamics (MD), are used to study these events. However, molecular dynamics simulations produce time series with multiple observables, while changes often only affect a few of them. Therefore, detecting conformational changes has proven to be challenging for most change-point detection algorithms. In this work, we focus on the identification of such events given many noisy observables. In particular, we show that the No-prior-Knowledge Exponential Weighted Moving Average (NEWMA) algorithm can be used along optical hardware to successfully identify these changes in real-time. Our method does not need to distinguish between the background of a protein and the protein itself. For larger simulations, it is faster than using traditional silicon hardware and has a lower memory footprint. This technique may enhance the sampling of the conformational space of molecules. It may also be used to detect change-points in other sequential data with a large number of features.Comment: 15 pages, 12 figure

Thermo-optical numerical modal analysis of multicore fibers for high power lasers and amplifiers

Lasers for modern industrial and research applications are required to provide a high average beam power and at the same time be reliable, efficient, and compact. Rare-earth doped single-mode fiber lasers are the most promising solution. Large mode area fibers are effectively used to reduce nonlinear effects and scaling up the beam power which is now bounded by thermal effects. A new cutting-edge approach to the issue involves the use of Multi-Core Fibers (MCFs), coherently combining several lower power beams into a higher power one, and thus pushing the threshold of nonlinearities and transverse mode instabilities to higher power. The amplification process involves heat generation in the doped cores due to quantum defect, which propagates radially and creates a temperature gradient across the fiber cross-section. Even though the cores are optically uncoupled, the refractive index gradient due to thermo-optical effects could cause cross-talk and core mode coupling. In this work, we numerically analyze the performances of 9-core MCFs for high power fiber lasers by taking into account the coupling and bending effects due to the heat load generated by the quantum defect between pump and laser radiation. MCFs show very low sensitivity to heat load and bending, with effectively single-mode behaviour up to 15 um core diameter (effective area 181 um2) and down to 35 um pitch

Enterobacteriaceae and Salmonella contamination of wild boar (Sus scrofa) carcasses: comparison between different sampling strategies

During 2020, a total of 64 wild boar carcasses were tested for Enterobacteriaceae count (EBC), Salmonella and Yersinia enterocolitica in the abdominal region (i) within 5 h after hunting in the game collection point and (ii) before dressing and processing in the game-handling establishment (GHE) (49 carcasses—average time interval between (i) and (ii): 4.3 days). Because of COVID-19 restrictions, 15 carcasses were transported to a near slaughterhouse (average time interval between (i) and (ii): 2.3 days). Mesenteric lymph nodes (MLNs) were collected and tested for Salmonella and Y. enterocolitica. Results are shown in relation to sampling A (49 carcasses—GHE) and sampling B (15 carcasses—slaughterhouse). Sampling A: EBC median values were (i) 2.51 log10 CFU/cm2 and (ii) 2.79 log10 CFU/cm2. EBC increase between (i) and (ii) was statistically significant (p = 0.001). Salmonella prevalence on carcasses varied from (i) 2.0 to (ii) 6.1%. Sampling B: EBC median values were (i) 3.1 log10 CFU/cm2 and (ii) 3.32 log10 CFU/cm2. EBC increase between (i) and (ii) was not statistically significant (p = 0.191). Salmonella prevalence on carcasses varied from (i) 6.7 to (ii) 0.0%. The prevalence (sampling A + B) of lymphatic Salmonella carriers was 7.8% (5/64). From carcasses and/or MNLs, the serovars Enteritidis, Typhimurium, Agama, Zaiman and Diarizonae O:50 (z) were detected. Y. enterocolitica was never isolated. Long chilling periods prior to wild game processing should be avoided, and carcasses should be tested at GHE rather than after shooting to proper reflect the microbial load of wild boar meat entering the food chain