Stratégie de réduction des micropolluants à l'échelle d'une agglomération : comparaison entre les effluents d’exutoires pluviaux et d’une station d'épuration

Colloque avec actes et comité de lecture. Internationale.International audienc

Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology

Modular and orthogonal genetic logic gates are essential for building robust biologically based digital devices to customize cell signalling in synthetic biology. Here we constructed an orthogonal AND gate in Escherichia coli using a novel hetero-regulation module from Pseudomonas syringae. The device comprises two co-activating genes hrpR and hrpS controlled by separate promoter inputs, and a σ54-dependent hrpL promoter driving the output. The hrpL promoter is activated only when both genes are expressed, generating digital-like AND integration behaviour. The AND gate is demonstrated to be modular by applying new regulated promoters to the inputs, and connecting the output to a NOT gate module to produce a combinatorial NAND gate. The circuits were assembled using a parts-based engineering approach of quantitative characterization, modelling, followed by construction and testing. The results show that new genetic logic devices can be engineered predictably from novel native orthogonal biological control elements using quantitatively in-context characterized parts

Liver transplantation for patients with human immunodeficiency virus and hepatitis C virus coinfection with special reference to hemophiliac recipients in Japan.

Liver transplantation for patients with hepatitis C virus (HCV) and human immunodeficiency virus (HIV) remains challenging. The advent of highly active antiretroviral therapy (HAART) for HIV has reduced mortality from opportunistic infection related to acquired immunodeficiency syndrome dramatically, while about 50% of patients die of end-stage liver cirrhosis resulting from HCV. In Japan, liver cirrhosis frequently develops after HCV-HIV coinfection resulting from previously transfused infected blood products for hemophilia. The problems of liver transplantation for those patients arise from the need to control calcineurin inhibitor with HAART drugs, the difficulty of using interferon after liver transplantation with HAART, and the need to control intraoperative coagulopathy associated with hemophilia. We review published reports of liver transplantation for these patients in the updated world literature

Is tibial cut navigation alone sufficient in medial unicompartmental knee arthroplasty? Continuous series of fifty nine procedures

International audiencePurpose: Our purpose was to assess medial unicompartmental knee arthroplasty with navigation alone for the tibial cut and limb alignment. We hypothesised that this technique could be used routinely in practice. Methods: Outcome measures were tibial cut orientation and residual varus. Six-month post-operative radiographs of 59 knees were assessed. Results: Tibial cut orientation was within 2° of planned in 70.2 and 76.3 % of knees in the coronal and sagittal planes, respectively (49.1 % in both), within 4° in 91.2 and 91.5 %, respectively (82.5 % in both). All coronal-plane errors were in varus. Excessive planed tibial slope was at risk of excessive varus of the tibial cut. The hip-knee-ankle angle was ≤179° in 81.4 % and the mechanical axis through Kennedy Zone 2 in 59.3 % of knees. Risk factors for inadequate varus were pre-operative hip-knee-ankle angle >176° and strictly articular varus. Conclusions: Our results are not as good as previously reported with this technique, but taking into account the factors of failure identified, we could enhance the results. © 2016, SICOT aisbl

AMIA Annu Symp Proc

Health data mining can bring valuable information for drug safety activities. We developed a visual analytics tool to find specific clinical event sequences within the data contained in a clinical data warehouse. To this aim, we adapted the Smith-Waterman DNA sequence alignment algorithm to retrieve clinical event sequences with a temporal pattern from the electronic health records included in a clinical data warehouse. A web interface facilitates interactive query specification and result visualization. We describe the adaptation of the Smith-Waterman algorithm, and the implemented user interface. The evaluation with pharmacovigilance use cases involved the detection of inadequate treatment decisions in patient sequences. The precision and recall results (F-measure = 0.87) suggest that our adaptation of the Smith-Waterman-based algorithm is well-suited for this type of pharmacovigilance activities. The user interface allowed the rapid identification of cases of inadequate treatment