93 research outputs found

    E. coli Nissle 1917 is a safe mucosal delivery vector for a birch-grass pollen chimera to prevent allergic poly-sensitization

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    International audienceAllergic poly-sensitization affects a large number of allergic patients and poses a great challenge for their treatment. In this study we evaluated the effects of the probiotic Escherichia coli Nissle 1917 (EcN) expressing a birch and grass pollen allergen chimera ‘Bet v 1, Phl p 1 and Phl p 5’ (EcN-Chim) on allergy prevention after oral or intranasal application in poly-sensitized mice. In contrast to oral application, intranasal pretreatment with EcN-Chim prior to poly-sensitization led to a significant reduction of lung inflammation (eosinophils, IL-5, and IL-13 in bronchoalveolar lavage) along with suppressed levels of allergen-specific serum IgE. The suppression was associated with increased levels of allergen-specific IgA in lungs and serum IgG2a along with increased Foxp3, TGF-ÎČ, and IL-10 mRNA in bronchial lymph nodes. In vitro EcN induced high levels of IL-10 and IL-6 in both lung and intestinal epithelial cells. Importantly, using in vivo imaging techniques we demonstrated that intranasally applied EcN do not permanently colonize nose, lung, and gut and this strain might therefore be a safe delivery vector against allergy in humans. In conclusion, our data show that intranasal application of recombinant EcN expressing a multiallergen chimera presents a novel and promising treatment strategy for prevention of allergic poly-sensitization

    Cross sections for exclusive channels in p‟\overline{p}p reactions at 12 GeV/c

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    The data obtained in an exposure of BEBC to an RF separated beam of antiprotons at 12 GeV/c are used to derive exclusive cross sections for reactions having one or more neutral strange particles in the final state. Particular attention is given to the estimation of the background. An upper limit of the cross section for the inclusive production of the charmed meson D* is also given

    Un corps pur

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    Se préoccuper de la pureté semble universellement répandu, mais les objets, les pratiques et les personnes considérés comme purs ou impurs varient selon les groupes, les sociétés et les époques, comme l'illustrent les huit articles de ce dossier thématiqu
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