Supplementary data for the article: Dinić, J.; Novaković, M.; Podolski-Renić, A.; Stojković, S.; Mandić, B.; Tešević, V.; Vajs, V.; Isaković, A.; Pešić, M. Antioxidative Activity of Diarylheptanoids from the Bark of Black Alder (Alnus Glutinosa) and Their Interaction with Anticancer Drugs. Planta Medica 2014, 80 (13), 1088–1096. https://doi.org/10.1055/s-0034-1382993

Supplementary material for: [https://doi.org/10.1055/s-0034-1382993]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1845

Supplementary material for the article: Dinić, J.; Novaković, M.; Podolski-Renić, A.; Vajs, V.; Tešević, V.; Isaković, A.; Pešić, M. Structural Differences in Diarylheptanoids Analogues from Alnus Viridis and Alnus Glutinosa Influence Their Activity and Selectivity towards Cancer Cells. Chemico-Biological Interactions 2016, 249, 36–45. https://doi.org/10.1016/j.cbi.2016.02.019

Supplementary material for: [https://doi.org/10.1016/j.cbi.2016.02.019]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2066

Supplementary data for the article: Novaković, M.; Pešić, M.; Trifunović, S.; Vučković, I.; Todorović, N.; Podolski-Renić, A.; Dinić, J.; Stojković, S.; Tešević, V.; Vajs, V.; et al. Diarylheptanoids from the Bark of Black Alder Inhibit the Growth of Sensitive and Multi-Drug Resistant Non-Small Cell Lung Carcinoma Cells. Phytochemistry 2014, 97, 46–54. https://doi.org/10.1016/j.phytochem.2013.11.001

Supplementary material for: [https://doi.org/10.1016/j.phytochem.2013.11.001]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1492

DTA0100, dual topoisomerase II and microtubule inhibitor, evades paclitaxel resistance in P-glycoprotein overexpressing cancer cells

The efficacy of microtubule targeting agents in cancer treatment has been compromised by the development of drug resistance that may involve both, P-glycoprotein overexpression and the changes in beta-tubulin isoforms' expression. The anti-Topoisomerase II activity of methyl 4-((E)-2-(methoxycarbonyl)vinyloxy)oct-2-ynoate (DTA0100) was recently reported. Herein, we further evaluated this propargylic enol ether derivative and found that it exerts inhibitory effect on tubulin polymerization by binding to colchicine binding site. DTA0100 mitotic arrest properties were investigated in two multi-drug resistant cancer cell lines with P-glycoprotein overexpression (colorectal carcinoma and glioblastoma). The sensitivity of multi-drug resistant cancer cell lines to DTA0100 was not significantly changed in contrast to microtubule targeting agents such as paclitaxel, vinblastine and colchicine. DTA0100 clearly induced microtubule depolymerization, leading to disturbance of cell cycle kinetics and subsequent apoptosis. The fine-tuning in beta-tubulin isoforms expression observed in multi drug resistant cancer cells may influence the efficacy of DTA0100. Importantly, DTA0100 blocked the Pglycoprotein function in both multi-drug resistant cancer cell lines without inducing the increase in Pglycoprotein expression. Therefore, DTA0100 acting as dual inhibitor of Topoisomerase II and microtubule formation could be considered as multi-potent anticancer agent. Besides, it is able to overcome the problem of drug resistance that emerges in the therapeutic approaches with either Topoisomerase II or microtubule targeting agents.Related to published version: [https://imagine.imgge.bg.ac.rs/handle/123456789/1079]This is the peer reviewed version of the paper: Podolski-Renić, A., Banković, J., Dinić, J., Rios-Luci, C., Fernandes, M. X., Ortega, N., Kovačević Grujičić, N., Martin, V. S., Padron, J. M., & Pesić, M. (2017). DTA0100, dual topoisomerase II and microtubule inhibitor, evades paclitaxel resistance in P-glycoprotein overexpressing cancer cells. European Journal of Pharmaceutical Sciences, 105, 159–168. [https://doi.org/10.1016/j.ejps.2017.05.011

Supplementary data for the article: Dinić, J.; Novaković, M.; Podolski-Renić, A.; Stojković, S.; Mandić, B.; Tešević, V.; Vajs, V.; Isaković, A.; Pešić, M. Antioxidative Activity of Diarylheptanoids from the Bark of Black Alder (Alnus Glutinosa) and Their Interaction with Anticancer Drugs. Planta Medica 2014, 80 (13), 1088–1096. https://doi.org/10.1055/s-0034-1382993

Supplementary material for: [https://doi.org/10.1055/s-0034-1382993]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1845

Supplementary data for the article: Jadranin, M.; Pešić, M.; Aljančić, I. S.; Milosavljević, S. M.; Todorović, N. M.; Podolski-Renić, A.; Banković, J.; Tanić, N.; Marković, I.; Vajs, V. E.; et al. Jatrophane Diterpenoids from the Latex of Euphorbia Dendroides and Their Anti-P-Glycoprotein Activity in Human Multi-Drug Resistant Cancer Cell Lines. Phytochemistry 2013, 86, 208–217. https://doi.org/10.1016/j.phytochem.2012.09.003

Supplementary material for: [https://doi.org/10.1016/j.phytochem.2012.09.003]Relate to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1585

Supplementary data for the article: Novaković, M.; Pešić, M.; Trifunović, S.; Vučković, I.; Todorović, N.; Podolski-Renić, A.; Dinić, J.; Stojković, S.; Tešević, V.; Vajs, V.; et al. Diarylheptanoids from the Bark of Black Alder Inhibit the Growth of Sensitive and Multi-Drug Resistant Non-Small Cell Lung Carcinoma Cells. Phytochemistry 2014, 97, 46–54. https://doi.org/10.1016/j.phytochem.2013.11.001

Supplementary material for: [https://doi.org/10.1016/j.phytochem.2013.11.001]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1492

Modulation of Antioxidant Potential with Coenzyme Q10 Suppressed Invasion of Temozolomide-Resistant Rat Glioma In Vitro and In Vivo.

The main reasons for the inefficiency of standard glioblastoma (GBM) therapy are the occurrence of chemoresistance and the invasion of GBM cells into surrounding brain tissues. New therapeutic approaches obstructing these processes may provide substantial survival improvements. The purpose of this study was to assess the potential of lipophilic antioxidant coenzyme Q10 (CoQ10) as a scavenger of reactive oxygen species (ROS) to increase sensitivity to temozolomide (TMZ) and suppress glioma cell invasion. To that end, we used a previously established TMZ-resistant RC6 rat glioma cell line, characterized by increased production of ROS, altered antioxidative capacity, and high invasion potential. CoQ10 in combination with TMZ exerted a synergistic antiproliferative effect. These results were confirmed in a 3D model of microfluidic devices showing that the CoQ10 and TMZ combination is more cytotoxic to RC6 cells than TMZ monotherapy. In addition, cotreatment with TMZ increased expression of mitochondrial antioxidant enzymes in RC6 cells. The anti-invasive potential of the combined treatment was shown by gelatin degradation, Matrigel invasion, and 3D spheroid invasion assays as well as in animal models. Inhibition of MMP9 gene expression as well as decreased N-cadherin and vimentin protein expression implied that CoQ10 can suppress invasiveness and the epithelial to mesenchymal transition in RC6 cells. Therefore, our data provide evidences in favor of CoQ10 supplementation to standard GBM treatment due to its potential to inhibit GBM invasion through modulation of the antioxidant capacity

Purine analogs sensitize the multidrug resistant cell line (NCl-H460/R) to doxorubicin

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Purine analogs sensitize the multidrug resistant cell line (NCl-H460/R) to doxorubicin

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