Aurora A kinase activity influences calcium signaling in kidney cells

Aurora A is abnormally expressed and activated in cells lining cysts associated with polycystic kidney disease and can phosphorylate and inactivate polycystin 2

Control system of parameters of the azimuthal module

Analytical and experimental studies of the azimuthal module of two-component vibrational micromechanical gyroscope were conducted. It is shown that the micromechanical gyroscope is a system with distributed parameters. The frequency analysis is performed using software T-Flex. The influence of mechanical disturbances on the movement of azimuthal module in the form of translational and angular oscillations is shown; the natural frequencies of the azimuth are defined

The analysis of toxic connections content in water by spectral methods

The current state of ecology means the strict observance of measures for the utilization of household and industrial wastes that is connected with very essential expenses of means and time. Thanks to spectroscopic devices usage the spectral methods allow to carry out the express quantitative and qualitative analysis in a workplace and field conditions. In a work the application of spectral methods by studying the degradation of toxic organic compounds after preliminary radiation of various sources is shown. Experimental data of optical density of water at various influences are given

Исповедный вопросник староверов Латгалии: лингвокультурный анализ текста

During the expedition to the Old Believers in Latgale (Latvia) a group of Slavists investigating the Russian language of Old Believers abroad got a scanned copy of the confessional questionnaire as a present from Basil Trishkin, a mentor of Gayok Old Believers’ House of Prayer (Daugavpils). This questionnaire is used by Basil Trishkin during confessions in conversations with the parishioners. As the study of the manuscript revealed the questionnaire according to its content, structure and features of language dates back to the pre-Raskol texts of the XѴII century. The next expedition, 2017, confirmed the assumption that the questionnaire is a part of the Old Believer’s prayer book. The expeditions did not pursue archeographic objectives, the conversations with mentors were mostly focused on prohibitions and prescriptions in the Old Believers’ community in Latgale for the purpose of its further sociolinguistic and ethnolinguistic analysis. Nevertheless the scanned manuscript certainly deserves attention as a piece of the Old Believers’ written culture. On the one hand, the Old Believer’s prayer book is written in Church Slavonic. On the other hand, the scanned manuscript is a source of information about the Latgalian Old Believers’ Russian language as a language for everyday communication, including lexical and other dialect features. The oral comments of the mentors Basil Trishkin and Ioann Zhilko about prohibitions in the Old Believers’ community, use of the questionnaire in the practice of worship and opportunities to pass it on to a group of researchers, with subsequent publication of the text, are particularly noteworthy.Во время экспедиции 2016 г. к староверам Латгалии (Латвия) группа славистов, исследователей русского языка старообрядцев за рубежом, получила в подарок от Василия Тришкина, наставника моленной беспоповцев на Гайкé (район г. Даугавпилса), сканированную копию вопросника, используемого им для исповеди прихожан. По мере исследования рукописи выяснилось, что вопросник по содержанию, структуре и особенностям языка восходит к текстам до никоновской справы XѴII в.; следующая экспедиция, 2017 г., подтвердила предположение, что вопросник находится в старообрядческом Требнике. Экспедиции не преследовали археографических целей, в беседах с наставниками обсуждались главным образом запреты староверов Латгалии с целью дальнейшего социолингвистического и этнолингвистического анализа. Однако попавший в распоряжение ученых исповедный вопросник, несомненно, заслуживает внимания как часть письменной культуры староверов и как источник сведений об используемом староверами Латгалии церковнославянском языке богослужения и русском языке повседневного общения, включающем лексические и иные диалектизмы, отраженные в тексте исповедного вопросника. Особую ценность представляют устные замечания наставников Василия Тришкина и Иоанна Жилко по поводу запретов староверов и использования самого вопросника в практике богослужения и возможностях передачи его группе исследователей с последующей публикацией текста

Choice of parameters and stability of nonlinear vibration isolation device

Work of active vibration isolation devices with single-mass electromagnetic suspension taking into account of real characteristics of the voltage regulators is described. The analytical researches are carried out; the areas of stability of work of nonlinear vibration isolation device are defined

Rapid calcium-dependent activation of Aurora-A kinase

Oncogenic hyperactivation of the mitotic kinase Aurora-A (AurA) in cancer is associated with genomic instability. Increasing evidence indicates that AurA also regulates critical processes in normal interphase cells, but the source of such activity has been obscure. We report here that multiple stimuli causing release of Ca2+ from intracellular endoplasmic reticulum stores rapidly and transiently activate AurA, without requirement for second messengers. This activation is mediated by direct Ca2+-dependent calmodulin (CaM) binding to multiple motifs on AurA. On the basis of structure–function analysis and molecular modelling, we map two primary regions of CaM-AurA interaction to unfolded sequences in the AurA N- and C-termini. This unexpected mechanism for AurA activation provides a new context for evaluating the function of AurA and its inhibitors in normal and cancerous cells

Draft Genome Sequences of Strains \u3ci\u3eSalinicola socius\u3c/i\u3e SMB35\u3csup\u3eT\u3c/sup\u3e, \u3ci\u3eSalinicola\u3c/i\u3e sp. MH3R3-1 and \u3ci\u3eChromohalobacter\u3c/i\u3e sp. SMB17 From the Verkhnekamsk Potash Mining Region of Russia

Halomonads are moderately halophilic bacteria that are studied as models of prokaryotic osmoadaptation and sources of enzymes and chemicals for biotechnological applications. Despite the progress in understanding the diversity of these organisms, our ability to explain ecological, metabolic, and biochemical traits of halomonads at the genomic sequence level remains limited. This study addresses this gap by presenting draft genomes of Salinicola socius SMB35T, Salinicola sp. MH3R3–1 and Chromohalobacter sp. SMB17, which were isolated from potash mine tailings in the Verkhnekamsk salt deposit area of Russia. The analysis of these genomes confirmed the importance of ectoines and quaternary amines to the capacity of halomonads to tolerate osmotic stress and adapt to hypersaline environments. The study also revealed that Chromohalobacter and Salinicola share 75–90% of the predicted proteome, but also harbor a set of genus-specific genes, which in Salinicola amounted to approximately 0.5 Mbp. These genus-specific genome segments may contribute to the phenotypic diversity of the Halomonadaceae and the ability of these organisms to adapt to changing environmental conditions and colonize new ecological niches

Mutational re-modeling of di-aspartyl intramembrane proteases: uncoupling physiologically-relevant activities from those associated with Alzheimer\u27s disease

The intramembrane proteolytic activities of presenilins (PSEN1/PS1 and PSEN2/PS2) underlie production of beta-amyloid, the key process in Alzheimer\u27s disease (AD). Dysregulation of presenilin-mediated signaling is linked to cancers. Inhibition of the gamma-cleavage activities of PSENs that produce Abeta, but not the epsilon-like cleavage activity that release physiologically essential transcription activators, is a potential approach for the development of rational therapies for AD. In order to identify whether different activities of PSEN1 can be dissociated, we designed multiple mutations in the evolutionary conserved sites of PSEN1. We tested them in vitro and in vivo assays and compared their activities with mutant isoforms of presenilin-related intramembrane di-aspartyl protease (IMPAS1 (IMP1)/signal peptide peptidase (SPP)). PSEN1 auto-cleavage was more resistant to the mutation remodeling than the epsilon-like proteolysis. PSEN1 with a G382A or a P433A mutation in evolutionary invariant sites retains functionally important APP epsilon- and Notch S3- cleavage activities, but G382A inhibits APP gamma-cleavage and Abeta production and a P433A elevates Abeta. The G382A variant cannot restore the normal cellular ER Ca(2+) leak in PSEN1/PSEN2 double knockout cells, but efficiently rescues the loss-of-function (Egl) phenotype of presenilin in C. elegans. We found that, unlike in PSEN1 knockout cells, endoplasmic reticulum (ER) Ca(2+) leak is not changed in the absence of IMP1/SPP. IMP1/SPP with the analogous mutations retained efficiency in cleavage of transmembrane substrates and rescued the lethality of Ce-imp-2 knockouts. In summary, our data show that mutations near the active catalytic sites of intramembrane di-aspartyl proteases have different consequences on proteolytic and signaling functions

The role of dual-energy computed tomography in the diagnosis of gout and other crystalline arthropathies: A review

The importance of dual-energy computed tomography in the diagnosis of gout, the principles of dual-energy computed tomography, the accuracy of the methodology, and the types of artifacts are discussed in this study. The possible dependence of the presence of deposits of sodium monourate on other clinical data and the role of dual-energy computed tomography in the differential diagnosis of other crystalline arthropathies are considered. The dual-energy computed tomography has several advantages, including noninvasiveness, speed of execution, and a significant reduction in the risk of iatrogenic consequences compared with diagnostic arthrocentesis, which is the gold standard in diagnosing gout. Dual-energy computed tomography can accomplish gout detection, treatment, and differential diagnosis