175 research outputs found
General Nonlinear 2-Fluid Hydrodynamics of Complex Fluids and Soft Matter
We discuss general 2-fluid hydrodynamic equations for complex fluids, where
one kind is a simple Newtonian fluid, while the other is either
liquid-crystalline or polymeric/elastomeric, thus being applicable to lyotropic
liquid crystals, polymer solutions, and swollen elastomers. The procedure can
easily be generalized to other complex fluid solutions. Special emphasis is
laid on such nonlinearities that originate from the 2-fluid description, like
the transport part of the total time derivatives. It is shown that the proper
velocities, with which the hydrodynamic quantities are convected, cannot be
chosen at will, since there are subtle relations among them. Within allowed
combinations the convective velocities are generally material dependent. The
so-called stress division problem, i.e. how the nematic or elastic stresses are
distributed between the two fluids, is shown to depend partially on the choice
of the convected velocities, but is otherwise also material dependent. A set of
reasonably simplified equations is given as well as a linearized version of an
effective concentration dynamics that may be used for comparison with
experiments
Structural basis for membrane insertion by the human ER membrane protein complex
A defining step in the biogenesis of a membrane protein is the insertion of its hydrophobic transmembrane helices into the lipid bilayer. The nine-subunit endoplasmic reticulum (ER) membrane protein complex (EMC) is a conserved co- and posttranslational insertase at the ER. We determined the structure of the human EMC in a lipid nanodisc to an overall resolution of 3.4 angstroms by cryo–electron microscopy, permitting building of a nearly complete atomic model. We used structure-guided mutagenesis to demonstrate that substrate insertion requires a methionine-rich cytosolic loop and occurs via an enclosed hydrophilic vestibule within the membrane formed by the subunits EMC3 and EMC6. We propose that the EMC uses local membrane thinning and a positively charged patch to decrease the energetic barrier for insertion into the bilayer
Convective Nonlinearity in Non-Newtonian Fluids
In the limit of infinite yield time for stresses, the hydrodynamic equations
for viscoelastic, Non-Newtonian liquids such as polymer melts must reduce to
that for solids. This piece of information suffices to uniquely determine the
nonlinear convective derivative, an ongoing point of contention in the rheology
literature.Comment: 4 page
Absence of surface mode in a visco-elastic material with surface tension
The surface waves in the visco-elastic media with the surface tension are
studied using the Voigt-Kelvin model of the visco-elasticity. It is shown that
the surface mode of oscillation does not exist in the parameter region where
the effect of surface tension is larger than that of the elastic stress at the
surface unless the viscous stress masks the elastic stress in the bulk. In the
region, the surface oscillation is suppressed and the oscillation beneath the
surface diffuses after the pulse goes into the bulk. The experimental relevance
of the present results is also discussed.Comment: 5 pages, 3 figure
Structural basis for membrane insertion by the human ER membrane protein complex
A defining step in the biogenesis of a membrane protein is the insertion of its hydrophobic transmembrane helices into the lipid bilayer. The nine-subunit endoplasmic reticulum (ER) membrane protein complex (EMC) is a conserved co- and posttranslational insertase at the ER. We determined the structure of the human EMC in a lipid nanodisc to an overall resolution of 3.4 angstroms by cryo–electron microscopy, permitting building of a nearly complete atomic model. We used structure-guided mutagenesis to demonstrate that substrate insertion requires a methionine-rich cytosolic loop and occurs via an enclosed hydrophilic vestibule within the membrane formed by the subunits EMC3 and EMC6. We propose that the EMC uses local membrane thinning and a positively charged patch to decrease the energetic barrier for insertion into the bilayer
Hydrodynamics of topological defects in nematic liquid crystals
We show that back-flow, the coupling between the order parameter and the
velocity fields, has a significant effect on the motion of defects in nematic
liquid crystals. In particular the defect speed can depend strongly on the
topological strength in two dimensions and on the sense of rotation of the
director about the core in three dimensions.Comment: 4 pages including two figure
Regulated assembly of the ER membrane protein complex
The assembly of nascent proteins into multi-subunit complexes is tightly regulated to maintain cellular homeostasis. The ER membrane protein complex (EMC) is an essential insertase that requires seven membrane-spanning and two soluble subunits for function. Here we show that the kinase With no lysine 1 (WNK1), known for its role in hypertension and neuropathy, is required for assembly of the human EMC. WNK1 uses a conserved amphipathic helix to stabilize the soluble subunit, EMC2, by binding to the EMC2-8 interface. Shielding this hydrophobic surface prevents promiscuous interactions of unassembled EMC2 and precludes binding of ubiquitin ligases, permitting assembly. Using biochemical reconstitution, we show that after EMC2 reaches the membrane, its interaction partners within the EMC displace WNK1, and similarly shield its exposed hydrophobic surfaces. This work describes an unexpected role for WNK1 in protein biogenesis, and defines the general requirements of an assembly factor that will apply across the proteome
WNK1 is an assembly factor for the human ER membrane protein complex
The assembly of nascent proteins into multi-subunit complexes is a tightly regulated process that must occur at high fidelity to maintain cellular homeostasis. The ER membrane protein complex (EMC) is an essential insertase that requires seven membrane-spanning and two soluble cytosolic subunits to function. Here, we show that the kinase with no lysine 1 (WNK1), known for its role in hypertension and neuropathy, functions as an assembly factor for the human EMC. WNK1 uses a conserved amphipathic helix to stabilize the soluble subunit, EMC2, by binding to the EMC2–8 interface. Shielding this hydrophobic surface prevents promiscuous interactions of unassembled EMC2 and directly competes for binding of E3 ubiquitin ligases, permitting assembly. Depletion of WNK1 thus destabilizes both the EMC and its membrane protein clients. This work describes an unexpected role for WNK1 in protein biogenesis and defines the general requirements of an assembly factor that will apply across the proteome
Faraday waves on a viscoelastic liquid
We investigate Faraday waves on a viscoelastic liquid. Onset measurements and
a nonlinear phase diagram for the selected patterns are presented. By virtue of
the elasticity of the material a surface resonance synchronous to the external
drive competes with the usual subharmonic Faraday instability. Close to the
bicriticality the nonlinear wave interaction gives rise to a variety of novel
surface states: Localised patches of hexagons, hexagonal superlattices,
coexistence of hexagons and lines. Theoretical stability calculations and
qualitative resonance arguments support the experimental observations.Comment: 4 pages, 4figure
Prolonged low flow reduces reactive hyperemia and augments low flow mediated constriction in the brachial artery independent of the menstrual cycle
© 2013 Rakobowchuk et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Non-invasive forearm ischemia-reperfusion injury and low flow induced vascular dysfunction models provide methods to
evaluate vascular function. The role of oestrogen, an endogenous anti-oxidant on recovery from ischemia-reperfusion injury
has not been evaluated nor has the impact of prolonged low flow on vascular function been established. Eight healthy women (33610 yr) attended the lab during the follicular, ovulatory and mid-luteal phases of their menstrual cycles. After 30 minutes of rest, brachial artery vascular function was assessed by ultrasound measurements of diameter changes during 5 minutes of forearm ischemia and 3 minutes after. Subsequently, a 20-minute forearm ischemia period was completed. Further, vascular function assessments were completed 15, 30 and 45 minutes into recovery. Flow-mediated dilation, lowflow-mediated constriction, and reactive hyperaemia proximal to the area of ischemia were determined. Flow-mediated dilation was reduced at 15 minutes of recovery but recovered at 30 and 45 minutes (PRE: 7.161.0%, POST15:4.560.6%, POST30:5. 560.7% POST45:5.960.4%, p,0.01). Conversely, low-flow mediated constriction increased (PRE: 21.360.4%, POST15: 23.360.6%, POST30: 22.560.5% POST45: 21.560.12%, p,0.01). Reactive hyperaemia was reduced throughout recovery (p,0.05). Data were unaffected by menstrual phase. Prolonged low flow altered vascular function and may relate as much to increased vasoconstriction as with decreased vasodilation. Reductions in anterograde shear and greater retrograde shear likely modulate the brachial artery response, but the reduced total shear also plays an important role. The data suggest substantial alterations in vascular function proximal to areas of ischemia with potential clinical implications following reperfusion.British Heart Foundation (PG/08/060/25340),a Physiological Society summer studentship to SG, and a Wellcome Trust Vacation Studentship to EP
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