277 research outputs found
Tardigrade Disordered Proteins as Potential Excipients for Biologics
Protein-based ‘biologics’ — drugs derived from living organisms — are among the most effective therapeutic treatments on the market. However, biologics are unstable, have short half-lives and require low temperature storage, making them prohibitively expensive. Although some biologics can be stabilized by formulation with excipients, most still require low temperature storage. In our search for new, more robust excipients, we turned to the tardigrade, a microscopic animal that synthesizes cytosolic abundant heat soluble (CAHS) proteins, a family unique to the tardigrade, to protect its cellular components during desiccation. This family of proteins protects lactate dehydrogenase against vacuum-drying induced inactivation and lipoprotein lipase against freeze-thaw and freeze-drying induced inactivation. Furthermore, CAHS proteins protect both enzymes more effectively than FDA approved excipients. CAHS proteins are intrinsically disordered with some transient structure in dilute solution. However, as they are concentrated, the transient structural elements are more likely to come into contact, resulting in gelation. When water is completely removed by lyophilization, CAHS proteins inhibit hydrogen-deuterium exchange of the protein GB1 with water vapor. Collectively, these studies lay the groundwork for understanding how CAHS proteins protect tardigrades against desiccation. Furthermore, these results support CAHS proteins as potential excipients for biologic drugs.Doctor of Philosoph
Mechanism of an ATP-independent Protein Disaggregase - I. Structure of a Membrane Protein Aggregate Reveals a Mechanism of Recognition by its Chaperone
Protein aggregation is detrimental to the maintenance of proper protein homeostasis in all cells. To overcome this problem, cells have evolved a network of molecular chaperones to prevent protein aggregation and even reverse existing protein aggregates. The most extensively studied disaggregase systems are ATP-driven macromolecular machines. Recently, we reported an alternative disaggregase system in which the 38-kDa subunit of chloroplast signal recognition particle (cpSRP43) efficiently reverses the aggregation of its substrates, the light-harvesting chlorophyll a/b-binding (LHC) proteins, in the absence of external energy input. To understand the molecular mechanism of this novel activity, here we used biophysical and biochemical methods to characterize the structure and nature of LHC protein aggregates. We show that LHC proteins form micellar, disc-shaped aggregates that are kinetically stable and detergent-resistant. Despite the nonamyloidal nature, the LHC aggregates have a defined global organization, displaying the chaperone recognition motif on its solvent-accessible surface. These findings suggest an attractive mechanism for recognition of the LHC aggregate by cpSRP43 and provide important constraints to define the capability of this chaperone
Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation
Tardigrades are microscopic animals that survive a
remarkable array of stresses, including desiccation.
How tardigrades survive desiccation has remained
a mystery for more than 250 years. Trehalose, a
disaccharide essential for several organisms to survive
drying, is detected at low levels or not at all in
some tardigrade species, indicating that tardigrades
possess potentially novel mechanisms for surviving
desiccation. Here we show that tardigrade-specific
intrinsically disordered proteins (TDPs) are essential
for desiccation tolerance. TDP genes are constitutively
expressed at high levels or induced during
desiccation in multiple tardigrade species. TDPs
are required for tardigrade desiccation tolerance,
and these genes are sufficient to increase desiccation
tolerance when expressed in heterologous systems.
TDPs form non-crystalline amorphous solids
(vitrify) upon desiccation, and this vitrified state mirrors
their protective capabilities. Our study identifies
TDPs as functional mediators of tardigrade desiccation
tolerance, expanding our knowledge of the roles
and diversity of disordered proteins involved in
stress tolerance
Substrate-Assisted Catalysis Unifies Two Families of Chitinolytic Enzymes
Hen egg-white lysozyme has long been the paradigm for enzymatic glycosyl hydrolysis with retention of configuration, with a protonated carboxylic acid and a deprotonated carboxylate participating in general acid-base catalysis. In marked contrast, the retaining chitin degrading enzymes from glycosyl hydrolase families 18 and 20 all have a single glutamic acid as the catalytic acid but lack a nucleophile on the enzyme. Both families have a catalytic (βα)8-barrel domain in common. X-ray structures of three different chitinolytic enzymes complexed with substrates or inhibitors identify a retaining mechanism involving a protein acid and the carbonyl oxygen atom of the substrate’s C2 N-acetyl group as the nucleophile. These studies unambiguously demonstrate the distortion of the sugar ring toward a sofa conformation, long postulated as being close to that of the transition state in glycosyl hydrolysis.
Development and introduction of recombinant factor VIII – a clinician’s experience
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/92006/1/hae2804.pd
Probing Synergy between Two Catalytic Strategies in the Glycoside Hydrolase O-GlcNAcase Using Multiple Linear Free Energy Relationships
Human O-GlcNAcase plays an important role in regulating the post-translational modification of serine and threonine residues with β-O-linked N-acetylglucosamine monosaccharide unit (O-GlcNAc). The mechanism of O-GlcNAcase involves nucleophilic participation of the 2-acetamido group of the substrate to displace a glycosidically linked leaving group. The tolerance of this enzyme for variation in substrate structure has enabled us to characterize O-GlcNAcase transition states using several series of substrates to generate multiple simultaneous free-energy relationships. Patterns revealing changes in mechanism, transition state, and rate-determining step upon concomitant variation of both nucleophilic strength and leaving group abilities are observed. The observed changes in mechanism reflect the roles played by the enzymic general acid and the catalytic nucleophile. Significantly, these results illustrate how the enzyme synergistically harnesses both modes of catalysis; a feature that eludes many small molecule models of catalysis. These studies also suggest the kinetic significance of an oxocarbenium ion intermediate in the O-GlcNAcase-catalyzed hydrolysis of glucosaminides, probing the limits of what may be learned using nonatomistic investigations of enzymic transition-state structure and offering general insights into how the superfamily of retaining glycoside hydrolases act as efficient catalysts
Effects of Anacetrapib in Patients with Atherosclerotic Vascular Disease
BACKGROUND:
Patients with atherosclerotic vascular disease remain at high risk for cardiovascular events despite effective statin-based treatment of low-density lipoprotein (LDL) cholesterol levels. The inhibition of cholesteryl ester transfer protein (CETP) by anacetrapib reduces LDL cholesterol levels and increases high-density lipoprotein (HDL) cholesterol levels. However, trials of other CETP inhibitors have shown neutral or adverse effects on cardiovascular outcomes.
METHODS:
We conducted a randomized, double-blind, placebo-controlled trial involving 30,449 adults with atherosclerotic vascular disease who were receiving intensive atorvastatin therapy and who had a mean LDL cholesterol level of 61 mg per deciliter (1.58 mmol per liter), a mean non-HDL cholesterol level of 92 mg per deciliter (2.38 mmol per liter), and a mean HDL cholesterol level of 40 mg per deciliter (1.03 mmol per liter). The patients were assigned to receive either 100 mg of anacetrapib once daily (15,225 patients) or matching placebo (15,224 patients). The primary outcome was the first major coronary event, a composite of coronary death, myocardial infarction, or coronary revascularization.
RESULTS:
During the median follow-up period of 4.1 years, the primary outcome occurred in significantly fewer patients in the anacetrapib group than in the placebo group (1640 of 15,225 patients [10.8%] vs. 1803 of 15,224 patients [11.8%]; rate ratio, 0.91; 95% confidence interval, 0.85 to 0.97; P=0.004). The relative difference in risk was similar across multiple prespecified subgroups. At the trial midpoint, the mean level of HDL cholesterol was higher by 43 mg per deciliter (1.12 mmol per liter) in the anacetrapib group than in the placebo group (a relative difference of 104%), and the mean level of non-HDL cholesterol was lower by 17 mg per deciliter (0.44 mmol per liter), a relative difference of -18%. There were no significant between-group differences in the risk of death, cancer, or other serious adverse events.
CONCLUSIONS:
Among patients with atherosclerotic vascular disease who were receiving intensive statin therapy, the use of anacetrapib resulted in a lower incidence of major coronary events than the use of placebo. (Funded by Merck and others; Current Controlled Trials number, ISRCTN48678192 ; ClinicalTrials.gov number, NCT01252953 ; and EudraCT number, 2010-023467-18 .)
Interaction of cellodextrins with lysozyme: The necessity of the 2-acetamido group for binding and hydrolysis
- …