Molecular investigation of CTX-M gene in Extended Spectrum β Lactamases (ESBLs) producing Pseudomonas aeruginosa isolated from Iranian patients with burn wound infection

Background: Pseudomonas aeruginosa (P. aeruginosa) is one of the most important causes of infection in burns and intensive care units. Extended-spectrum β-lactamases (ESBLs) production in P. aeruginosa is a major factor in the antibiotic resistance and is thought of as a serious threat to the currently available antibiotic armory. The purpose of this study was to determine the prevalence of CTX-M gene in ESBL-producing P. aeruginosa isolates in burn wound samples.Materials and methods: In this cross-sectional survey, a total of 60 clinical isolates of P. aeruginosa were obtained from patients suffering from burn wound infection referred to major hospitals of Tehran, Iran. After verification by biochemical tests and antimicrobial susceptibility testing, CTX-M gene was identified using PCR method.Results: The results of the molecular analysis of CTX-M gene showed that the prevalence of isolates of P. aeruginosa harboring CTX-M gene was 20% (12/60).Conclusion: The results from this study showed high levels of antibiotic resistance and CTX-M gene among P. aeruginosa isolated samples of burn-wound infections which condition may result in the increased the emergence of multidrug-resistant strains and the failure of therapy This study suggests that detailed data on the CTX-M gene frequency can be useful to achieve the best therapy for infections caused by ESBLs producing P. aeruginosa

A New Model for Automatic Text Classification

In this paper,a new method for automatic classification of texts is presented.This system includes two phases;text processing and text categorization.In the first phase,various indexing criteria such as bigram,trigram and quad-gram are presented to extract the properties.Then,in the second phase,the W-SMO machine learning algorithm is used to train the system.In order to evaluate and compare the results of the two criteria of accuracy and readability,Macro-F1 and Micro-F1 have been calculated for different indexing methods. The results of experiments performed on 7676 standard text documents of Reuters showed that the best performance is related to w-smo bigram criteria with accuracy of 95.17 micro and 79.86 macro.Also,the results indicated that our proposed method has the best performance compared to the W-j48,Naïve Bayes,K-NN and Decision Tree algorithms

The effect of perineal massage on labor second stage period perineal tear and its outcomes

Background and aims: Long liness second stage of labor may cause fatigue and discomfort for women. Most of the women experience perineal injury and perineal pain during vaginal delivery. Perineal pain makes the mother too weak to take care their babies. The aim of this study was to investigate the effectivness of perineal massage on labor secound stage period, intact perineal, episiotomy and intensity of perinal pain. Methods: This clinical trial study was performed on 141 nulliparous women (71 massages and 70 control group) who referred to Koohdasht Imam Khomeini hospital for normal vaginal delivery. Countinuous sampling method with random assignment was conducted. In massage group the there cervical dilatation (6-7) was used cm after dipping the pointing and middel fingers in to a lubricating substance (key jell) then dipping 4-5 cm offingers in to the vaginal of the parturing women massaging between 3 to 9 oclock alternatively. Questionnaire and code sheets were used for data collection. Data were analysied by SPSS version 14. Results: The mean length of second stage of labor in massage group was 58.16 ± 28.22 and in control group was 57.07 ± 28.93 minutes, the difference between the two groups was not significant (P=0.723). In the massage group the prevalauce of safe perineal, was 29.6%, episiotomy 32.4% and perineal tear was 38%. In the control group the prevalance of safe perineal was 11.4%, episiotomy 67.1% and perineal tear was 21.4%. Perineal pain on the third day of postpartum in massage group was 50.7% and in control group was 70% (P=0.019). Perineal pain on the tenth day postpartum in massage group was 29.6%, and in control group was 45.7% (P=0.048). There was a significant difference between the two groups comparing perineal pain, but there was no significant difference comparing the pain inteasty between the two groups (the third day P=0.036), (the thenth day P=0.390). Conclusion: The study shows that perineal massage is effective in increasing the likelihood of intact perineume and in decreasing the perineal pain

Irrigation with municipal wastewater as a suitable solution for safflower cultivation in arid regions

In order to study the effect of different manure and chemical fertilizer levels on qualitative and quantitative characteristics of safflower in the condition of irrigation with municipal wastewater, an experiment was conducted as split plot basis of randomized complete design in Lakhshah region locate in Zahedan city, Iran. The treatments were comprised of two levels of irrigation, W1= Well water and W2= Treated wastewater, in main plots and sub plots consisted of F1: control (without consumption of manure and chemical fertilizer), F2: Recommended manure, F3: Half of recommended manure and chemical fertilizer (N, P and K), and F4: Recommended chemical fertilizer (N, P and K). The results showed that Treatment of treated wastewater had a positive and significant influence on all yield components, and the most influence was shown on 1000 seed weight. Also, irrigation with wastewater significantly increases the dry and fresh yield and grain yield of safflower than ordinary water. Among the fertilizer treatments, complete treatment of chemical fertilizer N, P and K had the greatest effect on increase of yield and grain yield components. In this experiment, water treatment hadn’t significant effect on accumulation of Cr, pb, Fe and Mn in safflower grain, and soil. While, fertilizer treatment had only a significant influence on the accumulation of Fe and Mn in safflower grain, but between these treatments wasn’t saw any significant difference on the accumulation of Cr and pb. In general, the results of this experiment showed that irrigation with wastewater and application of complete fertilizer with manure is recommended

Status of decayed, missing, filled teeth index among Iranian children and adults: A systematic review and meta-analysis

Introduction: Oral health status is one of the health-related priorities in Iran. The present study was conducted with the aim to systematically review the status of oral health in Iran using the decayed, missing, filled teeth (DMFT) index. Methods: In this meta-analysis, all data available on DMFT were reviewed and 35 published studies were included in the study based on search strategy. To estimate overall DMFT and perform the meta-analyses, Comprehensive Meta-Analysis (CMA) software was used. Results: Of all 876 articles, 35 were included in the study. A total of 21849 individuals were included in the meta-analysis. The overall DMFT index was 3.65 [95% confidence interval (CI), 3.01-4.34]. In addition, this rate was 2.30, 8.60, and 3.85 among children, adults, and children with mental/physical disabilities with (CI 95%, 1.76-2.95), (CI 95%, 6.10-9.60), and (CI 95, 2.98-4.80), respectively. Conclusion: Mean DMFT in the assessed published data was higher than that set forth by the World Health Organization (WHO). Further planning and taking effective preventive measures to improve the oral health status seems necessary

Application of Response Surface Methodology to Optimize Malachite Green Removal by Cl-nZVI Nanocomposites

Disposal of effluents containing dyes into natural ecosystems pose serious threats to both the environment and its aquatic life. Malachite green (MG) is a basic dye that has extensive industrial applications, especially in aquaculture, throughout the world. This study reports on the application of the central composite design (CCD) under the response surface methodology (RSM) for the optimization of MG adsorption from aqueous solutions using the clinoptilolite nano-zerovalence iron (Cl-nZVI) nanocomposites. The sorbent structures produced are characterized by means of scanning electron micrograph (SEM), energy-dispersive X-ray spectroscopy (EDS), and vibrating sample magnetometer (VSM). The effects of different parameters including pH, initial MG concentration, and sorbent dosage on the removal efficiency (R) of MG were studied to find the optimum operating conditions. For this purpose, a total of 20 sets of experiments were designed by the Design Expert.7.0 software and the values of removal efficiency were used as input response to the software. The optimum pH, initial MG concentration, and sorbent dosage were found to be 5.6, 49.21 mg.L-1, and 1.43 g.L-1, respectively. A high MG removal efficiency (57.90%) was obtained with optimal process parameters. Moreover, a desirability value of 0.963 was obtained for the optimization process

Oral administration of DNA alginate nanovaccine induced immune-protection against Helicobacter pylori in Balb/C mice

Abstract Background Helicobacter pylori (H. Pylori), is an established causative factor for the development of gastric cancer and the induction of persistent stomach infections that may lead to peptic ulcers. In recent decades, several endeavours have been undertaken to develop a vaccine for H. pylori, although none have advanced to the clinical phase. The development of a successful H. pylori vaccine is hindered by particular challenges, such as the absence of secure mucosal vaccines to enhance local immune responses, the absence of identified antigens that are effective in vaccinations, and the absence of recognized indicators of protection. Methods The DNA vaccine was chemically cloned, and the cloning was verified using PCR and restriction enzyme digestion. The efficacy of the vaccination was investigated. The immunogenicity and immune-protective efficacy of the vaccination were assessed in BALB/c mice. This study demonstrated that administering a preventive Alginate/pCI-neo-UreH Nanovaccine directly into the stomach effectively triggered a robust immune response to protect against H. pylori infection in mice. Results The level of immune protection achieved with this nano vaccine was similar to that observed when using the widely accepted formalin-killed H. pylori Hel 305 as a positive control. The Alginate/pCI-neo-UreH Nanovaccine composition elicited significant mucosal and systemic antigen-specific antibody responses and strong intestinal and systemic Th1 responses. Moreover, the activation of IL-17R signaling is necessary for the defensive Th1 immune responses in the intestines triggered by Alginate/pCI-neo-UreH. Conclusion Alginate/pCI-neo-UreH is a potential Nanovaccine for use in an oral vaccine versus H. pylori infection, according to our findings

Early detection of Toxoplasma gondii by real-time polymerase chain reaction methods in patients with recurrent spontaneous abortions

Introduction: One of the causes of recurrent spontaneous abortions (RSA) is an infection by the toxoplasmosis Protozoa. In comparison, we present detailed results using real-time polymerase chain reaction (PCR) methods of detection. In this study, it was tried to detect Toxoplasma gondii (T. gondii) by real-time PCR methods in patients with RSA. Methods: Amniotic fluid sampling was performed in the 16-20th weeks of gestation in 50 pregnant women with a history of RSA. The extracted deoxyribonucleic acid (DNA) samples were analyzed using quantitative real-time PCR. Results: In all the cases, the detection of T. gondii was negative in the peripheral blood, and amniotic fluid samples by using the molecular methods (real-time PCR). Using the serological detection methods, 6% of patients were diagnosed as positive for the immunoglobulin M (IgM) antibody. In addition, the IgG antibody was positive in 46% of the patients. Conclusion: It can be concluded that the serological methods lack specificity

Retracted: Novel NAD‐independent Avibacterium paragallinarum: Isolation, characterization and molecular identification in Iran

Abstract Background Infectious coryza (IC) is an invasive upper respiratory disease caused by Avibacterium paragallinarum that affects birds, particularly chickens. The objective of this study is to isolate, characterize and molecularly identify the bacterium A. paragallinarum in poultry birds, as well as to determine its antibiotic sensitivity and resistance. Methods A total of 10 chickens from four different Iranian farms with typical IC symptoms were used in this study. The nasal swabs were streaked onto chocolate agar plates and blood agar plates and incubated at 37°C in 5% CO2 for 24 to 48 h. As part of the identification of bacteria, bacteriological observations and polymerase chain reaction (PCR) testing are conducted. The antibiotic sensitivity tests were also performed using the disk diffusion method against A. paragallinarum and the prevalence in different farms was determined. Results By using biochemical assays and PCR analyses, seven strains of A. paragallinarum were isolated from samples of four chicken farms with typical IC clinical signs. Most isolates (4/7) showed the typical requirement for nicotinamide adenine dinucleotide (NAD) and an enriched CO2 atmosphere for growth. Three of the seven strains of A. paragallinarum were found to be novel NAD‐independent under anaerobic conditions. There was one biochemical biovar identified in terms of carbohydrate fermentation patterns, although changes in maltose carbohydrate fermentation patterns were detected in the No. 5 strain. All isolates were sensitive to gentamicin and spectinomycin. Three novel NAD‐independent strains (Nos.1, 5 and 7) were found to be multidrug‐resistant (MDR) and resistant to at least three classes of antibiotics. There was greater antibiotic resistance in the three NAD‐independent isolates than in normal NAD‐dependent bacteria. Conclusion By discovering NAD‐independent forms of A. paragallinarum, these species have a greater range than previously believed. A clear, cautious approach should be taken in diagnosing and possibly controlling IC