Heterochrony, generic distinction and phylogeny in the family Hydractiniidae (Hydrozoa, Cnidaria)

The taxonomy of Hydractinia, Stylactaria and Podocoryna is discussed and the three genera are merged into Hydractinia since their diagnostic characters are liable to lead to polyphyly and paraphyly, due to repeated episodes of medusa reduction via heterochrony (paedomorphosis). The phylogeny of the Hydractiniidae is reconstructed by using two outgroups, Clava and Cytaeis, both having some characters in common with the Hydractiniidae. The resulting phylogenetic trees agree in identifying affinities among Hydractinia, Kinetocodium and Hydrocorella, all with polymorphic colonies with gastrozooids having oral tentacles. The position of Clavactinia (characterized by gastrozooids with widely scattered tentacles) is at the root of the tree if Clava is the outgroup, whereas it becomes apical when the outgroup is Cytaeis. The pattern of medusa suppression is different in the two cladograms, since the presence of a medusa is a plesiomorphic feature when Cytaeis is the outgroup, whereas it becomes apomorphic when the outgroup is Clava. These inconveniences are difficult to accommodate, since medusa suppression has occurred many times in the evolution of the hydroidomedusae, and Recent species do not witness past paedomorphic events of medusa reduction properly, so that many intermediate states are probably missing

The broad band spectral properties of binary X-ray pulsars

The X-ray telescopes on board BeppoSAX are an optimal set of instruments to observe bright galactic binary pulsars. These sources emit very hard and quite complex X-ray spectra that can be accurately measured with BeppoSAX between 0.1 and 200 keV. A prototype of this complexity, the source Her X-1, shows at least seven different components in its spectrum. A broad band measure is therefore of paramount importance to have a thorough insight into the physics of the emitting region. Moreover the detection of cyclotron features, when present, allows a direct and highly significant measure of the magnetic field intensity in the emission region. In this paper we briefly report the results obtained with BeppoSAX on this class of sources, with emphasis on the detection and on the measured properties of the cyclotron lines.Comment: 10 Latex pages, 4 figures, uses psfig.sty. Accepted for publication in Advances in Space Research, in Proceedings of 32nd Scientific Assembly of COSPAR - Symposium E1.1: "Broad-Band X-ray Spectroscopy of Cosmic Sources

Project "Biodiversity MARE Tricase": a biodiversity inventory of the coastal area of Tricase (Ionian Sea, Italy) – Mollusca: Heterobranchia

The marine biodiversity of the Tricase coastal area (Ionian Sea, Italy) was investigated at the MARE Outpost (Avamposto MARE) between 2016 and 2017, with the help of citizen scientists and trained taxonomists. Among the most interesting groups encountered, heterobranch molluscs deserve a special mention. Altogether, 268 specimens were ascribed to this group and referred to 49 taxa. Notwithstanding the extensive literature on Mediterranean heterobranchs, two species proved to be new to the Italian coasts, the records of eight species represented their easternmost sightings in the Mediterranean Sea, and 13 taxa represented new records for the Ionian Sea. In addition, several feeding habits and phenological events were new to science. Although the European Union's Marine Strategy Framework Directive lists "Biodiversity is maintained" as the first descriptor in achieving "Good Environmental Status", our results highlighted the presence of conspicuous gaps in the knowledge of species distribution, taxonomy and ecology of heterobranch molluscs, indicating the necessity of even small-scale species checklists to understand biodiversity changes in worldwide biota

Dendroecology of Aspidosperma polyneuron Müll. Arg. (Apocynaceae) trees in two different geomorphological growth conditions in Atlantic forests, Paraná State, Brazil.

Ameridendro

Microfabricated polyester conical microwells for cell culture applications.

Over the past few years there has been a great deal of interest in reducing experimental systems to a lab-on-a-chip scale. There has been particular interest in conducting high-throughput screening studies using microscale devices, for example in stem cell research. Microwells have emerged as the structure of choice for such tests. Most manufacturing approaches for microwell fabrication are based on photolithography, soft lithography, and etching. However, some of these approaches require extensive equipment, lengthy fabrication process, and modifications to the existing microwell patterns are costly. Here we show a convenient, fast, and low-cost method for fabricating microwells for cell culture applications by laser ablation of a polyester film coated with silicone glue. Microwell diameter was controlled by adjusting the laser power and speed, and the well depth by stacking several layers of film. By using this setup, a device containing hundreds of microwells can be fabricated in a few minutes to analyze cell behavior. Murine embryonic stem cells and human hepatoblastoma cells were seeded in polyester microwells of different sizes and showed that after 9 days in culture cell aggregates were formed without a noticeable deleterious effect of the polyester film and glue. These results show that the polyester microwell platform may be useful for cell culture applications. The ease of fabrication adds to the appeal of this device as minimal technological skill and equipment is required

A dendrochronological analysis of Pinus pinea L. on the Italian mid-Tyrrhenian coast

In order to assess the response of the radial growth of Pinus pinea L. to climatic variability in Central Italy, dendrochronological and dendroclimatological analyses were carried out on five different populations scattered along the Tyrrhenian coasts of the peninsula. The aim of this study is to contribute to the understanding of the ecological demands of this species, particularly in the study area. For each site total ring, early-, and late-wood width chronologies were developed. Multidimensional analyses were performed for the three tree-ring datasets in order to analyze the relations between sites chronologies. Both Principal Component Analyses and hierarchical classifications highlighted an important difference of one site in respect to the other, probably due to site characteristics. Correlation functions were performed to infer the main climatic factors controlling the radial growth of the species. For a comparative study, we limited our attention to the common interval 1926-2003 (78 years) in which the response of the tree-ring chronologies to climate at both local and regional scale was investigated. Positive moisture balance in the late spring-summer period of the year of growth is the climatic driver of P. pinea radial growth in the study area. Moreover, this study shows how low summer temperatures strongly favor the radial growth of the species.Fil: Piraino, Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto Argentino de Investigaciones de Zonas Aridas; ArgentinaFil: Camiz, Sergio. Universita degli studi di Roma "Sapienza". Dipartimento di Matematica; ItaliaFil: Di Filippo, Alfredo. Universita Degli Studi Della Tuscia. DendrologyLab; ItaliaFil: Piovesan, Gianluca. Universita Degli Studi Della Tuscia. DendrologyLab; ItaliaFil: Spada, Francesco. Universita degli studi di Roma "Sapienza". Dipartimento de biología ambientale; Itali

Discovery of a Hard X-Ray Source, SAX J0635+0533, in the Error Box of the Gamma-Ray Source 2EG 0635+0521

We have discovered an x-ray source, SAX J0635+0533, with a hard spectrum within the error box of the GeV gamma-ray source in Monoceros, 2EG J0635+0521. The unabsorbed x-ray flux is 1.2*10^-11 erg cm^-2 s^-1 in the 2-10 keV band. The x-ray spectrum is consistent with a simple powerlaw model with absorption. The photon index is 1.50 +/- 0.08 and we detect emission out to 40 keV. Optical observations identify a counterpart with a V-magnitude of 12.8. The counterpart has broad emission lines and the colors of an early B type star. If the identification of the x-ray/optical source with the gamma-ray source is correct, then the source would be a gamma-ray emitting x-ray binary.Comment: Accepted to the Astrophysical Journal, 8 page