41 research outputs found

    Do Temporal and Spatial Parameters or Lifestyle of the Pacific Oyster Crasssostrea gigas Affect Pollutant Bioaccumulation, Offspring Development, and Tolerance to Pollutants?

    Get PDF
    International audienceThis study evaluated the vulnerability of early life stages of native oysters (Crassostrea gigas) from the Arcachon Bay (SW, France) to pollutants at risk in the lagoon in particular copper and S-metolachlor. Developmental abnormalities in wild and cultivated oyster D-larvae were investigated during 2 breeding-seasons (2013 and 2014) at different sampling sites and dates. In addition, copper, and metolachlor concentrations were determined both in seawater and in mature oysters. Bioaccumulation of Cu was observed at higher levels in wild than in farmed specimens. Metolachlor was accumulated at much lower levels. After 24 h exposure, significant increases of the percentage of abnormal D-larvae were observed when exposed at 1 ÎŒg L−1 of copper or 10 ng L−1 of metolachlor in comparison with the controls whatever the date, the site, and rearing conditions of the genitors. The current study demonstrates that environmental concentrations of copper and metolachlor can induce a significant increase of developmental abnormalities in farmed and wild populations of oysters. However, no significant differences of sensitivity were observed according to temporal, spatial parameters, and lifestyle of genitors. In addition, oyster larvae obtained from the hatchery displayed the same sensitivity to pollutants as larvae from the field, demonstrating their suitability for toxicity assays and water quality monitoring

    First assessment of Atlantic open ocean Sargassum spp. metal and metalloid concentrations

    Get PDF
    International audienceOver the last decade, increasing proliferations of Atlantic Sargassum populations have led to massive beaching with disastrous environmental consequences. This study is a preliminary assessment of open ocean Sargassum spp. element concentration to assess their potential contribution on coastal ecosystems. Sargassum spp. samples from seven sites, collected along a transect from the center of the Atlantic Ocean to near the coast of Martinique (French West Indies), were analyzed to determine their potential metal and metalloid contamination. Mean element concentrations from the Sargassum spp. samples were ranked in the following descending order: As > Fe > Mn > Al > Zn > V > Ni > Cu > Cr > Cd > Hg. Element concentrations are relatively low compared to previous results of beached Sargassum spp. except for As that need to be carefully considered before reusing Sargassum spp

    Corbicula fluminea: A sentinel species for urban Rare Earth Element origin

    No full text
    International audienceThe increase in the global population, coupled with growing consumption of Rare Earth Elements (REEs), has led to increasing transfer of these emerging contaminants into the environment, particularly through the effluents from wastewater treatment plants (WWTP). The objectives of this study were to determine the geochemical quality of a French river subject to strong urban pressure (the Jalle River in the Bordeaux area) and to examine the bioavailability of natural and anthropogenic REEs in a model species of freshwater bivalve, the Asian clam Corbicula fluminea. To this end, two fractions (dissolved and total) of the water from the Jalle River were sampled and the bivalves were exposed by in situ caging during a three-month monitoring period. The REE patterns obtained showed the presence of Gadolinium (Gd) anomalies in the dissolved and total fractions as well as in Corbicula fluminea. The apparent bioavailability of natural REEs was in the following order for the dissolved fraction: Medium REEs (MREEs) > Light REEs (LREEs) > Heavy REEs (HREEs) and for the particulate fraction: MREEs > LREEs = HREEs. These results highlight the importance of the particulate fraction in the study of the bioavailability of REEs in bivalves. An increase of anthropogenic Gd (Gdanth) was observed in the dissolved fraction between the upstream site (3.4 ng.L−1) and the WWTP Downstream site (48.4 ng.L−1). The Gd anomaly observed in the water was also observed in Corbicula fluminea with a significant increase in the bioaccumulation of Gdanth, from 1.5 ± 1 ng.gDW−1 upstream to 4.1 ± 0.7 ng.gDW−1 downstream of the WWTP effluents, thus confirming the enhanced bioavailability of medical-origin Gd to freshwater bivalves. This study strongly suggests that Corbicula fluminea can be used as a sentinel species in the monitoring of Gd contamination of medical origin. It would thus appear important to consider the potential entry of this contaminant into the human food chain via other, commercially exploited bivalve species

    Comparison of the accumulation and effects of copper pyrithione and copper sulphate on rainbow trout larvae

    No full text
    Copper pyrithione (CuPT) is used as a co-biocide in new antifouling paints but its toxicity remains little known. To compare the toxicity of copper-based compounds, rainbow trout (Oncorhynchus mykiss) larvae were exposed for 8-day to CuPT and CuSO at equivalent copper concentrations. CuPT exposure led to the greatest accumulation of Cu in larvae. Exposure to 10 ”g.L CuPT induced 99% larval mortality but only 4% for CuSO-exposed larvae. The larval development and growth were affected by CuPT (from 0.5 ”g.L Cu) but not by CuSO. Lipid peroxidation was not induced by either contaminant. The expression of genes involved in oxidative stress defence, detoxification and copper transport was induced in larvae exposed to CuSO and CuPT but at higher concentrations for CuPT. This study highlights the marked toxicity of CuPT for early life stages of fish and raises the question of the possible environmental risks of this antifouling compound

    Sensitivity of newly transformed juveniles of the freshwater pearl mussel: Margaritifera margaritifera to acute toxicity of a wide range of contaminants

    No full text
    The freshwater pearl mussel (FWPM) Margaritifera margaritifera is a bivalve mollusk critically endangered in Europe. There is a special concern about the conservation of this species, but little is known about its sensitivity to environmental pollution. Here, acute toxicity tests were conducted at 16°C according to a standard guide for conducting toxicity tests on freshwater mussels (ASTM international E2455), in order to assess toxicity thresholds for arsenic (As), aluminum (Al), cadmium (Cd), copper (Cu), nickel (Ni), nitrates (NO3−), and orthophosphates (PO43−) on newly transformed juveniles of M. margaritifera. The comparison with toxicity data for other newly transformed freshwater mussel juveniles from the same family and superfamily, Margaritiferidae and Unionidae, showed that FWPM juveniles were the most sensitive species to NO3−, among the most sensitive species to Ni, the least sensitive species to Cd, and showed an intermediate sensitivity to Cu compared to other freshwater mussels. This study is the first to provide toxicity thresholds for several pollutants on newly transformed juveniles of the FWPM. This brings new knowledge on its sensitivity to environmental pollution that could be helpful data for conservation strategies and these findings represent the first window for further complex studies

    Sub-lethal effects of waterborne copper in early developmental stages of rainbow trout (Oncorhynchus mykiss)

    Get PDF
    International audienceThe aim of this work was to study the impact of copper during a sub-chronic exposure to environmental concentrations in the early life stages of rainbow trout (Oncorhynchus mykiss). Eyed-stage embryos of rainbow trout, at 265°D, were exposed in semi-static conditions to sublethal concentrations of CuSO4 up to the larval stage (528 °D) under laboratory-controlled conditions. During 3 weeks, they were exposed to the environmentally-realistic concentration of 2 ”g/L Cu and to a 10-fold higher concentration, 20 ”g/L Cu. Several biological (survival, hatching success, malformation, growth) and behavioral (swimming activity) and molecular endpoints (genotoxicity and gene transcription) were studied. Exposure to 20 ”g/L Cu had an inhibitory effect on hatching and increased half-hatched embryos (25%). At the end of the exposure, no significant differences were observed in growth of the larvae exposed to the highest Cu concentration. However, larvae exposed to 2 ”g/L Cu exhibited increased growth in comparison with non-exposed larvae. The percentage of malformed larvae was significantly higher for both copper conditions, with skeletal malformations being the most observed. Expression of several genes was evaluated in whole larvae using quantitative real-time PCR. Genes involved in detoxification (gst, mt1 and mt2) and in cell cycle arrest (p53) were significantly repressed in both copper conditions when compared to control. In addition, potential genotoxic effects on larvae were investigated by the comet assay on blood cells, but this test did not demonstrate any significant DNA damage on larvae exposed to copper. This study confirms the adverse effects of copper on early life stages of rainbow trout even at the lowest environmentally relevant tested concentration
    corecore