Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos-7

<p><b>Copyright information:</b></p><p>Taken from "Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos"</p><p>http://www.biomedcentral.com/1471-213X/7/116</p><p>BMC Developmental Biology 2007;7():116-116.</p><p>Published online 18 Oct 2007</p><p>PMCID:PMC2169233.</p><p></p>dard sample. The results are presented as proportion of blastocysts with or without detectable transcripts in expressing blastocysts (Figure ). The relative RNA level is expressed according to the equation: RNA quantity = 2 with ΔΔCt = (Ct()-Ct()- Ct()-Ct()). For each group, individual blastocyst expression is represented. The bars indicate the median expression value. The size of symbols is proportional to blastocyst number with the same expression (Figure )

Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos-1

<p><b>Copyright information:</b></p><p>Taken from "Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos"</p><p>http://www.biomedcentral.com/1471-213X/7/116</p><p>BMC Developmental Biology 2007;7():116-116.</p><p>Published online 18 Oct 2007</p><p>PMCID:PMC2169233.</p><p></p>ically observed (2 PN) and defined the zygote stage. Zygotes obtained after fertilization (Figure group D) or after fertilization (Figure group C) were then cultured in M16 or G1.2/G2.2 medium. The results are expressed as percentage of total zygote (PN) number at day 0 for each experimental group and each culture condition. At each culture day, the number of atretic embryos was determined based upon the observation of necrosis signs

Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos-2

<p><b>Copyright information:</b></p><p>Taken from "Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos"</p><p>http://www.biomedcentral.com/1471-213X/7/116</p><p>BMC Developmental Biology 2007;7():116-116.</p><p>Published online 18 Oct 2007</p><p>PMCID:PMC2169233.</p><p></p>rect sequencing (Figure ) and cloning/sequencing (Figure ). When Single Nucleotide Polymorphism (C/T) was observed by direct sequencing, the proportion of C in the clone sequences was approximately 50%. When only C or T were detected by direct sequencing, all sequences of analyzed clones presented a methylated or an unmethylated status respectively. Reading the direct sequences, for each blastocyst, the presence of C/T, C or T at one CpG position is represented by the black (methylated) and white (unmethylated) lollipops (Figure )

Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos-0

<p><b>Copyright information:</b></p><p>Taken from "Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos"</p><p>http://www.biomedcentral.com/1471-213X/7/116</p><p>BMC Developmental Biology 2007;7():116-116.</p><p>Published online 18 Oct 2007</p><p>PMCID:PMC2169233.</p><p></p>y and degree of expansion). The results were expressed as a percentage of the total blastocyst number. In the same experimental group (C or D), significant differences of maturity degree were observed according to the culture medium (χtest, P < 0.05

Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos-4

<p><b>Copyright information:</b></p><p>Taken from "Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos"</p><p>http://www.biomedcentral.com/1471-213X/7/116</p><p>BMC Developmental Biology 2007;7():116-116.</p><p>Published online 18 Oct 2007</p><p>PMCID:PMC2169233.</p><p></p> CTCF binding sites are shaded in grey. Only examples are shown for clarity

Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos-6

<p><b>Copyright information:</b></p><p>Taken from "Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos"</p><p>http://www.biomedcentral.com/1471-213X/7/116</p><p>BMC Developmental Biology 2007;7():116-116.</p><p>Published online 18 Oct 2007</p><p>PMCID:PMC2169233.</p><p></p

Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos-3

<p><b>Copyright information:</b></p><p>Taken from "Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos"</p><p>http://www.biomedcentral.com/1471-213X/7/116</p><p>BMC Developmental Biology 2007;7():116-116.</p><p>Published online 18 Oct 2007</p><p>PMCID:PMC2169233.</p><p></p>ation defects (Figure 6B) are shown

Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos-8

<p><b>Copyright information:</b></p><p>Taken from "Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos"</p><p>http://www.biomedcentral.com/1471-213X/7/116</p><p>BMC Developmental Biology 2007;7():116-116.</p><p>Published online 18 Oct 2007</p><p>PMCID:PMC2169233.</p><p></p>y and degree of expansion). The results were expressed as a percentage of the total blastocyst number. In the same experimental group (C or D), significant differences of maturity degree were observed according to the culture medium (χtest, P < 0.05

Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos-5

<p><b>Copyright information:</b></p><p>Taken from "Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos"</p><p>http://www.biomedcentral.com/1471-213X/7/116</p><p>BMC Developmental Biology 2007;7():116-116.</p><p>Published online 18 Oct 2007</p><p>PMCID:PMC2169233.</p><p></p>thylation defects (Figure ) are shown