Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos-2
<p><b>Copyright information:</b></p><p>Taken from "Assisted Reproductive Technology affects developmental kinetics, Imprinting Control Region methylation and gene expression in individual mouse embryos"</p><p>http://www.biomedcentral.com/1471-213X/7/116</p><p>BMC Developmental Biology 2007;7():116-116.</p><p>Published online 18 Oct 2007</p><p>PMCID:PMC2169233.</p><p></p>rect sequencing (Figure ) and cloning/sequencing (Figure ). When Single Nucleotide Polymorphism (C/T) was observed by direct sequencing, the proportion of C in the clone sequences was approximately 50%. When only C or T were detected by direct sequencing, all sequences of analyzed clones presented a methylated or an unmethylated status respectively. Reading the direct sequences, for each blastocyst, the presence of C/T, C or T at one CpG position is represented by the black (methylated) and white (unmethylated) lollipops (Figure )
