Elongation, rooting and acclimatization of micropropagated shoots from mature material of hybrid larch

Factors were defined for elongation, rooting and acclimatization of micropropagated shoots of Larix x eurolepis Henry initiated from short shoot buds of plagiotropic stecklings serially propagated for 9 years from an 8-year-old tree. Initiation and multiplication were on Schenk and Hildebrandt (SH) medium supplemented with 5 μM 6-benzyladenine (BA) and 1 μM indole-butyric acid (IBA). Stem elongation was obtained in 36% of the shoots on SH medium containing 0.5 μM BA and 63% of the remaining non-elongated shoots initiated stem elongation after transfer on SH medium devoid of growth regulators. Rooting involved 2 steps: root induction on Campbell and Durzan mineral salts and Murashige and Skoog organic elements, both half-strength (CD-MS/2), supplemented with 1 μM of both naphthaleneacetic acid (NAA) and IBA, and root elongation following transfer to CD-MS/2 medium devoid of growth regulators. Repeating this 2-step sequence yielded up to 67% rooted shoots. Acclimatization of plantlets ranged from 83% to 100%. Over 300 plants were transferred to the greenhouse; some showed plagiotropic growth

An Insight to Micropropagation of Freshwater Aquatic Medicinal Plants

Freshwater aquatic plants include medicinal and ornamental species: have limited demand and rarely micropropagated. Most of them are found in Southeast Asian environments as amphibians or fully submerged or floating in lakes, streams, and watercourses that are ignored as weeds. These plants have never been central focuses and were not looked for characteristic proliferation. It is difficult to multiply these plants using traditional techniques through seeds or natural proliferation of rhizomes or cuttings by maintaining quality. Rare and fragmented but important information about micropropagation of these neglected plants has been discussed in this chapter. There are certain aquatic or semiaquatic medicinal plants for which no effort has been made to propagate in vitro. Contrarily, plants like water hyssop have extensive in vitro regeneration protocols due to its high demand. This study compares methodologies used by researchers to micropropagate these plants. It is concluded that this study will help in understanding and establishing systems for scientific propagation of these plants. © Springer Nature Switzerland AG 2019