Evaluation of time difference of arrival (Tdoa) networks performance for launcher vehicles and spacecraft tracking

Time Difference of Arrival (TDOA) networks could support spacecraft orbit determination or near-space (launcher and suborbital) vehicle tracking for an increased number of satellite launches and space missions in the near future. The evaluation of the geometry of TDOA networks could involve the dilution of precision (DOP), but this parameter is related to a single position of the target, while the positioning accuracy of the network with targets in the whole celestial vault should be evaluated. The paper presents the derivation of the MDOP (minimum dilution of precision), a parameter that can be used for evaluating the performance of TDOA networks for spacecraft tracking and orbit determination. The MDOP trend with respect to distance, number of stations and target altitude is reported in the paper, as well as examples of applications for network performance evaluation or time precision requirement definitions. The results show how an increase in the baseline enables the inclusion of more impactive improvements on the MDOP and the mean error than an increase in the number of stations. The target altitude is demonstrated as noninfluential for the MDOP trend, making the networks uniformly applicable to lower altitude (launchers and suborbital vehicles) and higher altitude (Low and Medium Earth Orbits satellites) spacecraft

LEO object’s light-curve acquisition system and their inversion for attitude reconstruction

In recent years, the increase in space activities has brought the space debris issue to the top of the list of all space agencies. The fact of there being uncontrolled objects is a problem both for the operational satellites in orbit (avoiding collisions) and for the safety of people on the ground (re-entry objects). Optical systems provide valuable assistance in identifying and monitoring such objects. The Sapienza Space System and Space Surveillance (S5Lab) has been working in this field for years, being able to take advantage of a network of telescopes spread over different continents. This article is focused on the re-entry phase of the object; indeed, the knowledge of the state of the object, in terms of position, velocity, and attitude during the descent, is crucial in order to predict as accurately as possible the impact point on the ground. A procedure to retrieve the light curves of orbiting objects by means of optical data will be shown and a method to obtain the attitude determination from their inversion based on a stochastic optimization (genetic algorithm) will be proposed

Optical observations for energetic characterization of in-orbit explosion: the FREGAT-SB case

Over the past years, the constant increase of space debris and inactive satellites is the root cause of catastrophic events, such as collision between a debris and active satellites. One of the events that might generate a large number of debris is the in-orbit explosion. Within this complex framework, it is of paramount importance to use a monitoring and surveillance system in order to understand the number and the distribution of fragments, in an area around the Earth extremely populated by man-made object. This entails a growing international interest in Space Surveillance and Tracking (SST), where optical observation reaches an interesting method to obtain information of orbital objects. In this paper, the Sapienza Space Systems and Space Surveillance Laboratory (S5Lab) presents the results of an observative campaign focused on the energetical characterization of the explosion and the monitoring of the fragments, which have been generated by a low orbit explosion of the third Russian stage rocket FREGAT-SB (ID 37756). The event occurred on 08.05.2020 between 04:00 and 06:00 UTC time. Through the observatory system Sapienza Coupled University Debris Observatory (SCUDO) located in Collepardo (FR, Italy), a certain number of images have been collected. The observation strategy was focused on the orbital plane to try to estimate the number of these fragments and their distribution. Once the astrometry phase to retrieve the measures in terms of right ascension and declination was performed, a first analysis is carried on to understand whether or not the fragments are already present in the North American Aerospace Defense Command (NORAD) catalogue. The ones that are not catalogued could be FREGAT’s fragments. The next energetic characterization method is based on a tangential impulse assignment in agreement with isotropic explosion and the evolution of fragments’ cloud, where the important variations, to a first approximation, are on semiaxes and eccentricity. As a result of this procedure, an association between the impulse and the measure takes place. The analysis of the angular distance between original body and the fragments over the time is carried out, in order to validate this method. Moreover, a magnitude estimation procedure is shown. All these results are compared with those obtained with the NORAD assignment

Non-coding RNAs as prognostic markers for endometrial cancer

Endometrial cancer (EC) has been classified over the years, for prognostic and therapeutic purposes. In recent years, classification systems have been emerging not only based on EC clinical and pathological characteristics but also on its genetic and epigenetic features. Noncoding RNAs (ncRNAs) are emerging as promising markers in several cancer types, including EC, for which their prognostic value is currently under investigation and will likely integrate the present prognostic tools based on protein coding genes. This review aims to underline the importance of the genetic and epigenetic events in the EC tumorigenesis, by expounding upon the prognostic role of ncRNAs

Towards personalized medicine: Non-coding rnas and endometrial cancer

Endometrial cancer (EC) is the most frequent female cancer associated with excellent prognosis if diagnosed at an early stage. The risk factors on which clinical staging is based are constantly updated and genetic and epigenetic characteristics have recently been emerging as prognostic markers. The evidence shows that non-coding RNAs (ncRNAs) play a fundamental role in various biological processes associated with the pathogenesis of EC and many of them also have a prognosis prediction function, of remarkable importance in defining the therapeutic and surveillance path of EC patients. Personalized medicine focuses on the continuous updating of risk factors that are identifiable early during the EC staging to tailor treatments to patients. This review aims to show a summary of the current classification systems and to encourage the integration of various risk factors, introducing the prognostic role of non-coding RNAs, to avoid aggressive therapies where not necessary and to treat and strictly monitor subjects at greater risk of relapse

Synergic stimulation of serotonin 5-HT1A receptor and α2-adrenoceptors for neuropathic pain relief: Preclinical effects of 2-substituted imidazoline derivatives

Neuropathic pain affects millions of people causing disability and impairing quality of life. Commonly used analgesics are generally characterized by limited therapeutic outcomes. The serotonin 5-HT1A receptor and the α2 adrenergic receptors are involved in central nociceptive mechanisms with a pivotal role in the inhibitory descending pain pathway. Since their stimulation may modulate the nervous signaling altered by neuropathies, the purpose of the present research is the study of the combined activation of 5-HT1A and α2 receptors by rationally designed imidazoline ligands ((S)-(-)-1 and 2-5) in a rat model of neuropathic pain (chronic constriction injury - CCI). On day 14 after nerve damage, the acute administration per os (p.o.) of low doses of (S)-(-)-1 (0.1-1mg/kg) was able to significantly increase the pain threshold to mechanical noxious stimuli for more than 1h. (S)-(-)-1 efficacy was confirmed by the decrease of spontaneous pain evaluated as hind limb weight bearing alterations. The clinically-used compound gabapentin (100mg/kg p.o.) induced a pain relieving effect similar to (S)-(-)-1 administered at 100 fold lower dose. In the same model, the selected analogues, compounds 2-5 (1mg/kg p.o.) were effective 30min after administration. In particular, 5 fully reverted the CCI-induced hypersensitivity. The pain relieving activity of 5 was significantly prevented by the selective 5-HT1A receptor antagonist WAY 100635 (1mg/kg intraperitoneally, i.p.) and, at a lesser extent, by the α2 antagonist yohimbine (3mg/kg i.p.). A novel pharmacodynamic approach to the treatment of neuropathic pain is presented

Non-coding RNAs and endometrial cancer

Non-coding RNAs (ncRNAs) are involved in the regulation of cell metabolism and neoplastic transformation. Recent studies have tried to clarify the significance of these information carriers in the genesis and progression of various cancers and their use as biomarkers for the disease; possible targets for the inhibition of growth and invasion by the neoplastic cells have been suggested. The significance of ncRNAs in lung cancer, bladder cancer, kidney cancer, and melanoma has been amply investigated with important results. Recently, the role of long non-coding RNAs (lncRNAs) has also been included in cancer studies. Studies on the relation between endometrial cancer (EC) and ncRNAs, such as small ncRNAs or micro RNAs (miRNAs), transfer RNAs (tRNAs), ribosomal RNAs (rRNAs), antisense RNAs (asRNAs), small nuclear RNAs (snRNAs), Piwi-interacting RNAs (piRNAs), small nucleolar RNAs (snoRNAs), competing endogenous RNAs (ceRNAs), lncRNAs, and long intergenic ncRNAs (lincRNAs) have been published. The recent literature produced in the last three years was extracted from PubMed by two independent readers, which was then selected for the possible relation between ncRNAs, oncogenesis in general, and EC in particular

Y RNA: an overview of their role as potential biomarkers and molecular targets in human cancers

Y RNA are a class of small non-coding RNA that are largely conserved. Although their discovery was almost 40 years ago, their function is still under investigation. This is evident in cancer biology, where their role was first studied just a dozen years ago. Since then, only a few contributions were published, mostly scattered across different tumor types and, in some cases, also suffering from methodological limitations. Nonetheless, these sparse data may be used to make some estimations and suggest routes to better understand the role of Y RNA in cancer formation and characterization. Here we summarize the current knowledge about Y RNA in multiple types of cancer, also including a paragraph about tumors that might be included in this list in the future, if more evidence becomes available. The picture arising indicates that Y RNA might be useful in tumor characterization, also relying on non-invasive methods, such as the analysis of the content of extracellular vesicles (EV) that are retrieved from blood plasma and other bodily fluids. Due to the established role of Y RNA in DNA replication, it is possible to hypothesize their therapeutic targeting to inhibit cell proliferation in oncological patients

Novel Potent Muscarinic Receptor Antagonists: Investigation on the Nature of Lipophilic Substituents in the 5- and/or 6-Positions of the 1,4-Dioxane Nucleus

A series of novel 1,4-dioxane analogues of the muscarinic acetylcholine receptor (mAChR) antagonist 2 was synthesized and studied for their affinity at M1-M5 mAChRs. The 6-cyclohexyl-6-phenyl derivative 3b, with a cis configuration between the CH2N+(CH3)3 chain in the 2-position and the cyclohexyl moiety in the 6-position, showed pKi values for mAChRs higher than those of 2 and a selectivity profile analogous to that of the clinically approved drug oxybutynin. The study of the enantiomers of 3b and the corresponding tertiary amine 33b revealed that the eutomers are (2S,6S)-(-)-3b and (2S,6S)-(-)-33b, respectively. Docking simulations on the M3 mAChR-resolved structure rationalized the experimental observations. The quaternary ammonium function, which should prevent the crossing of the blood-brain barrier, and the high M3/M2 selectivity, which might limit cardiovascular side effects, make 3b a valuable starting point for the design of novel antagonists potentially useful in peripheral diseases in which M3 receptors are involved

Usage of Light Emitting Diodes (LEDs) for improved satellite tracking

With the increasing number of satellite launches, especially in Low Earth Orbit (LEO), optical tracking can offer a convenient enhancement of tracking precision and availability. Spaceborne active illumination devices, such as LED payloads, can offer a significant improvement to optical observations, extending the observability interval to the whole eclipse time and performing optimized flash sequences for identification, orbit determination, attitude reconstruction or low data rate communication. The main features of LED panels for optical tracking mounted on small satellites platforms (and with particular regards to nano-satellite platforms) are outlined in this paper, along with the description of the design drivers. The analysis of the performance is referred to Sun-Synchronous (at 700 km of altitude) and International Space Station (400 km) orbits, while the ground segment and the optical link budget reference design relies on a standard university space debris observation station architecture. The paper also outlines the advantages of using different observation techniques and the variety of flashing patterns. The LEDSAT 1U CubeSat, aiming at demonstrating the effectiveness of an LED-based payload for observation and tracking, is used as a study case for examples of the LED payloads and related operations that are reported and described in this paper