Direction of transcription of two ribosomal protein genes in Escherichia coli: J.Mol.Biol.

NRC publication: Ye

Genetic position and amino acid replacements of several mutations in ribosomal protein S5 from Escherichia coli

NRC publication: Ye

Stabilitaet und Verbleib von rekombinanten Organismen bzw. Nucleinsaeuren in Forschungslabors und unter industriellen Produktionsbedingungen Abschlussbericht

As a contribution to unanswered safety questions when employing genetic modified organisms the following developments are presented: (i) specific oligonucleotide probes and PCR samples for the detection of genetically engineered streptomyces, (ii) isolation and characterization of dimethyl sulfoxide degradating oxidase/reductase from Hyphomicrobium sp. and specific probes/PCR primers for Hyphomicrobium, (iii) new shuttle vectors for E. coli and Streptomyces containing polylinkers and lacZ casettes for blue/white selection on x-gal plates in E. coli, (iv) cloning and sequence analysis of the mel operons for melanin biosynthesis from Streptomyces. Research is continued on the expression of human kallikrein protein as a model system for expression and spreading in actinomycetes and on the development of gene probes for dimethyl sulfoxide degradating genes. (WEN)SIGLEAvailable from TIB Hannover: F96B688+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Kloniersysteme fuer Streptomyceten Proteinsekretionssysteme und Entwicklung proteasearmer Wirtsstaemme. Schlussbericht

Gentechnology became recently available for Streptomycetes, especially for excreted proteins. The long-lasting experience in Streptomyces fermentation asks for efforts to use these bacteria as hosts for overproduction of excretable enzymes. Protease-poor strains should be screened. Genetic engineering tools and methods for use with S. griseus N2-3-11 and S. galbus DSM 40480 have been developed. Genes for protease A and B and of the StrK phosphatase were cloned from S. griseus N2-3-11 and expressed in S. lividans 66 and S. galbus DSM 40480. Signalpeptide sequences were used for construction of expression-cassettes in streptomycetes. Heterologous gene expression in streptomycetes will be useful in future and the project therefore will be continued. (orig.)Gentechnische Methoden sind seit kurzem fuer Streptomyceten, besonders fuer exkretierte Proteine, verfuegbar. Die lange Erfahrung in der Fermentationstechnologie der Streptomyceten macht Anstrengungen zur Entwicklung dieser Bakteriengruppe als Wirtssysteme fuer die heterologe Proteinexkretion erforderlich. Die Gentechnik fuer die Staemme S. griseus N2-3-11 und S. galbus DSM 40480 wurde entwickelt. Die Gene fuer Protease A und B sowie der StrK Phosphatase (extrazellulaere Enzyme) wurden aus S. griseus kloniert und in S. lividans 66 und S. galbus DSM 40480 exprimiert. Signalpeptidsequenzen wurden fuer die Konstruktion von Expressionskassetten in Streptomyceten verwendet. Die heterologe Genexpression wird in Streptomyceten moeglich sein und sollte in dem fortgefuehrten Vorhaben weiterentwickelt werden. (orig.)Available from TIB Hannover: D.Dt.F. AC1000(44,10) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman