Stabilitaet und Verbleib von rekombinanten Organismen bzw. Nucleinsaeuren in Forschungslabors und unter industriellen Produktionsbedingungen Abschlussbericht

Abstract

As a contribution to unanswered safety questions when employing genetic modified organisms the following developments are presented: (i) specific oligonucleotide probes and PCR samples for the detection of genetically engineered streptomyces, (ii) isolation and characterization of dimethyl sulfoxide degradating oxidase/reductase from Hyphomicrobium sp. and specific probes/PCR primers for Hyphomicrobium, (iii) new shuttle vectors for E. coli and Streptomyces containing polylinkers and lacZ casettes for blue/white selection on x-gal plates in E. coli, (iv) cloning and sequence analysis of the mel operons for melanin biosynthesis from Streptomyces. Research is continued on the expression of human kallikrein protein as a model system for expression and spreading in actinomycetes and on the development of gene probes for dimethyl sulfoxide degradating genes. (WEN)SIGLEAvailable from TIB Hannover: F96B688+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman