Development of a pathogenicity testing system for Dothistroma pini infection of Pinus radiata : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology at Massey University

Dothistroma pini is a fungal pathogen of pine species around the world and can be found in most parts of New Zealand. Infection by D. pini causes a disease commonly known as Dothistroma needle blight. Dothistroma needle blight has a significant financial impact on New Zealand's forestry industry. Although control of infection by D. pini is currently very successful there is a possibility that a new strain introduced from another country could be a lot more damaging and overcome current control measures. In recent years both the incidence and severity of the disease have increased in the northern hemisphere and other parts of the world. A distinctive characteristic of Dothistroma needle blight is the production in the infected needle of a toxic red pigment called dothistromin. Dothistromin is produced as a secondary metabolite by D. pini and has known phytotoxic properties as well as clastogenic and mutagenic properties towards human cells. Purified dothistromin toxin injected into pine needles has been shown to reproduce symptoms similar to those observed during D. pini infection. Because of this production, dothistromin is thought to play an important role in the infection process. Mutants of D. pini that are deficient in dothistromin production have been made recently that will allow this role to be investigated. The aim of this study was to develop a pathogenicity testing system under PC2 containment (required for dothistromin deficient mutant) and to develop microscopy methods required to monitor both epiphytic and endophytic growth of the fungus on the needle D. pini requires high light intensity, continuous leaf moisture and a specific temperature range in order to infect pine needles. Progress was made towards developing a robust pathogenicity testing system. This study has also developed several microscopy techniques for the visualisation of epiphytic growth including a fluorescent microscopy technique. Other bright field and fluorescent staining techniques were investigated with some success. Staining techniques were not successful for the visualisation of endophytic D. pini growth but a green fluorescent protein (sgfp) reporter construct was obtained and two gfp plasmid contracts were developed for the transformation of D. pini for use as biomarkers. Successful introduction of the gfp constructs into D. pini will allow in situ visualisation of endophytic and epiphytic D. pini growth. The work done in this study will be useful for the further investigation into the role of dothistromin toxin, which may lead to new or more efficient methods of controlling D. pini as well as possibly providing information about other polyketide molecules of economic or medical significance

Creating a functional musician: a performance workshop model

This paper examines the innovative re-alignment of one Australian tertiary music program in response to economic imperatives and a rapidly evolving marketplace. A 'functional musician' is technically sound, versatile, adaptive, collaborative, empathetic, creative, and capable of adapting to most professional situations. Conservatoire training models designed to produce classical musicians specialising in performance or education do not necessarily meet industry needs in twenty-first century Australia. Following changes to the secondary school music curriculum, undergraduate students are arriving at University with different musical skills. Responding to these changes, the University of Southern Queensland has adapted its tertiary classical music degree programs to create a new 'workshop model' for Music Practice courses to produce employable music graduates with adaptable skills suitable for the diverse Australian musical sector. The new model had its initial implementation in semester 1 (February-June) 2012. Data was collected from two student surveys and from student reflective journals; it is intended that these form the beginning of a longitudinal survey. Analysis of the initial data indicates the workshop model is successful in many of its aims, but shows some areas needing refinement

Transition, agriculture and the vanishing market

No abstract is available for this item

A non-technical introduction to the ANEMMarket model of the Australian National Electricity Market (NEM)

In this paper, we provide an accessible introduction to our agent-based ANEMMarket simulation model of the Australian National Electricity Market. This model has been purpose built to assess the impacts of emissions trading schemes, carbon taxes and the introduction of significant new suppliers of electricity generated from low or zero carbon emitting generators. We provide an illustrative example that involves the simulation of the impacts of a range of carbon prices on the dispatch of power from different types of generators in different regional locations. From these we compute the resultant carbon reduction effects. However, these remain only illustrative simulations because they do not include a range of operative constraints that exist in reality.

Modelling the effects of subsistence on Bulgarian agricultural performance

No abstract is available for this item

Regime-switching Vector Error Correction Model (VECM) analysis of UK meat consumption

The asymptotic distributions of cointegration tests are approximated using the Gamma distribution. The tests considered are for the I(1), the conditional I(1), as well as the I(2) model. Formulae for the parameters of the Gamma distributions are derived from response surfaces. The resulting approximation is flexible, easy to implement and more accurate than the standard tables previously published