2 research outputs found
Vaping Induced Cannabidiol (CBD) Oxidation Product CBD Quinone Forms Protein Adducts with KEAP1 and Activates KEAP1-Nrf2 Genes
Cannabidiol (CBD) vaping products have become widely
available
in the U.S. since their legalization in 2018. However, little is known
about their respiratory health effects. Here we show that aerosolization
of commercial CBD vaping products generates a reactive CBD quinone
(CBDQ) which forms adducts with protein cysteine residues. Using click
chemistry and a novel in vitro vaping product exposure
system (VaPES), we further demonstrate that CBDQ forms adducts with
human bronchial epithelial cell proteins including Keap1 and activates
KEAP1-Nrf2 stress response pathway genes. These results suggest that
vaping CBD alters protein function and induces cellular stress pathways
in the lung
Gene Expression Profiling in Human Lung Cells Exposed to Isoprene-Derived Secondary Organic Aerosol
Secondary
organic aerosol (SOA) derived from the photochemical
oxidation of isoprene contributes a substantial mass fraction to atmospheric
fine particulate matter (PM<sub>2.5</sub>). The formation of isoprene
SOA is influenced largely by anthropogenic emissions through multiphase
chemistry of its multigenerational oxidation products. Considering
the abundance of isoprene SOA in the troposphere, understanding mechanisms
of adverse health effects through inhalation exposure is critical
to mitigating its potential impact on public health. In this study,
we assessed the effects of isoprene SOA on gene expression in human
airway epithelial cells (BEAS-2B) through an air–liquid interface
exposure. Gene expression profiling of 84 oxidative stress and 249
inflammation-associated human genes was performed. Our results show
that the expression levels of 29 genes were significantly altered
upon isoprene SOA exposure under noncytotoxic conditions (<i>p</i> < 0.05), with the majority (22/29) of genes passing
a false discovery rate threshold of 0.3. The most significantly affected
genes belong to the nuclear factor (erythroid-derived 2)-like 2 (Nrf2)
transcription factor network. The Nrf2 function is confirmed through
a reporter cell line. Together with detailed characterization of SOA
constituents, this study reveals the impact of isoprene SOA exposure
on lung responses and highlights the importance of further understanding
its potential health outcomes