Early metabolic defects in dexamethasone-exposed and undernourished intrauterine growth restricted rats.

Poor fetal growth, also known as intrauterine growth restriction (IUGR), is a worldwide health concern. IUGR is commonly associated with both an increased risk in perinatal mortality and a higher prevalence of developing chronic metabolic diseases later in life. Obesity, type 2 diabetes or metabolic syndrome could result from noxious "metabolic programming." In order to better understand early alterations involved in metabolic programming, we modeled IUGR rat pups through either prenatal exposure to synthetic glucocorticoid (dams infused with dexamethasone 100 µg/kg/day, DEX) or prenatal undernutrition (dams feeding restricted to 30% of ad libitum intake, UN). Physiological (glucose and insulin tolerance), morphometric (automated tissue image analysis) and transcriptomic (quantitative PCR) approaches were combined during early life of these IUGR pups with a special focus on their endocrine pancreas and adipose tissue development. In the absence of catch-up growth before weaning, DEX and UN IUGR pups both presented basal hyperglycaemia, decreased glucose tolerance, and pancreatic islet atrophy. Other early metabolic defects were model-specific: DEX pups presented decreased insulin sensitivity whereas UN pups exhibited lowered glucose-induced insulin secretion and more marked alterations in gene expression of pancreatic islet and adipose tissue development regulators. In conclusion, these results show that before any catch-up growth, IUGR rats present early physiologic, morphologic and transcriptomic defects, which can be considered as initial mechanistic basis of metabolic programming

PCR primers for quantitative real-time PCR.

<p>PCR primers for quantitative real-time PCR.</p

Glucose homeostasis in IUGR pups.

<p>Phosphoenolpyruvate carboxykinase (Pck1) and glucose-6-phosphatase (G6pc) mRNA (A) and protein (B and C) levels in liver at PND7, circulating glucose (D), insulin (E) and C-peptide (F) levels during a GTT at PND21, relative glycaemia during an ITT at PND28 (G) of male control pups (CON), prenatally dexamethasone-exposed pups (DEX) and prenatally undernourrished pups (UN). N = 6 male rats per group for panel A, N = 4 male rats per group for panel B and C, N = 12–15 male rats per group for panel D and G, N = 10–12 male rats per group for panel E and F. For all panel, * p≤0.05 or indicated value for DEX vs. CON group, # p≤0.05 for UN vs. CON group and § p≤0.05 for DEX vs. UN group.</p

Adipose tissue deposition and transcriptional changes in IUGR pups at weaning.

<p>Weight of epididymal white adipose tissue (eWAT) (A), mRNA levels of transcription factors in eWAT (B), mRNA levels of adipokines in eWAT (C), weight of interscapular brown adipose tissue (iBAT) (D), mRNA levels of markers in iBAT (E), circulating leptin levels (F) in male control pups (CON), prenatally dexamethasone-exposed pups (DEX) and prenatally undernourrished pups (UN) at PND21. N = 10 (panel A–E), N = 5 (panel F) male rats per group, * p≤0.05 for DEX vs. CON group, # p≤0.05 for UN vs. CON group for all panels.</p

Morphological analysis of endocrine pancreas in IUGR pups.

<p>Islet area reported to whole pancreas area (A), number of islets per section (B), mean islet size expressed in µm² (C) and number of small (300–5000µm²), medium (5000–10000µm²) and large (>10000µm²) islets per section (D) of male control pups (CON), prenatally dexamethasone-exposed pups (DEX) and prenatally undernourrished pups (UN). For all panel, N = 5–6 male rats per group, 3 pancreatic sections per rat, * p≤0.02 for DEX vs. CON group, # p≤0.005 for UN vs. CON group (A), * p≤0.005 for DEX vs. CON group, # p≤0.001 for UN vs. CON group (B), * p≤0.0001 for DEX vs. CON group, # p≤0.01 for UN vs. CON group (C), * p≤0.05 for DEX vs. CON group, # p≤0.05 for UN vs. CON group (D).</p

The effects of dexamethasone exposure and undernutrition on prenatal and postnatal maternal weight.

<p>Body weight curve during gestation (A) and post-delivery during lactation (B), circulating leptin levels (C) and circulating corticosterone levels (D) of control dams (CON), dexamethasone-exposed dams (DEX) and undernourrished dams (UN). Circulating leptin and corticosterone levels were measured at gestational day 19 (G19). N = 12–15 gestating rats per group, * p≤0.05 for DEX vs. CON group and # p≤0.05 for UN vs. CON group for panel A. N = 5 gestating rats per group, * p≤0.05 for DEX vs. CON group and # p≤0.05 for UN vs. CON group for panel B. N = 5 gestating rats per group, * p≤0.01 for DEX vs. CON group and # p≤0.002 for UN vs. CON group for panel C. N = 7–9 gestating rats per group, * p≤0.001 for DEX vs. CON group and # p≤0.05 for UN vs. CON group for panel D.</p