Intestinal Tritrichomonas foetus infection in cats in Switzerland detected by in vitro cultivation and PCR

Tritrichomonas foetus, a parasite well known for its significance as venereally transmitted pathogen in cattle, has recently been identified as a cause of chronic large-bowel diarrhea in domestic cats in the US, UK, and, more recently, also in Norway. In a period of 3months (October to December 2007), 45 cats of Switzerland suffering from chronic diarrhea were investigated for intestinal infections, including a search for trichomonads. A commercially available in vitro culture system was used to screen for infection, complemented with a PCR and subsequent amplicon sequencing to support speciation. The PCR is based upon amplification of a sequence derived from the internal transcribed spacer region 1 (ITS1) on the ribosomal RNA gene (rRNA) using primers designed to detect a broad range of genera and species belonging to the family of Trichomonadidae. The method was furthermore adapted to the uracil DNA glycosylase (UDG) system in order to prevent carry-over contamination and it included a recombinant internal control to track for inhibitory reactions. Eleven out of the 45 cats were culture-positive, as revealed by microscopic identification of trichomonadid organisms. One of the isolates was subjected to scanning electron microscopy and findings revealed the presence of three flagella, thus placing the isolate into the gender Tritrichomonas sp. PCR and subsequent amplicon sequencing were carried out with ten of the 11 isolates. A total homology with published T. foetus sequences was confirmed in all of the cases. T. foetus therefore appears to range among those organisms that can cause chronic diarrhea in cats in Switzerlan

Humanitarian protection as a european public good: the strategic role of states and refugees

The surge of refugees arriving in Europe has accentuated the malfunctioning of the common European asylum system: the lack of coordination between nation states and failure in the common protection of refugees were the main outcomes of the so‐called refugee crisis. This article builds on the literature on public goods and policy compliance in order to explain the failure of European countries to provide humanitarian protection to refugees. A sequential game‐theoretical model serves to demonstrate the strategic interaction between states and refugees in European asylum policy. The analysis demonstrates that although both groups of actors benefit from a functioning European asylum system, they also have few incentives to contribute to the public good. States aim to reduce their individual refugee burden and refugees seek protection in their preferred destination country. The findings suggest that an effective provision of refugee protection requires both member states and refugees to contribute mutually to the public good

Responsibility-sharing in refugee protection: lessons from climate governance

The international governance of asylum requires states to cooperate to provide the public good of humanitarian protection. The need to establish responsibility-sharing resembles the collective action problem in climate change mitigation. While there is a general consensus on the differentiation of state responsibilities in most environmental agreements, states continuously fail to progress on responsibility-sharing in asylum governance. In this article, we compare the collective action challenges in asylum to those in climate governance and identify the similarities and differences in their characteristics as public goods. We then discuss the principle of “common but differentiated responsibilities” that guides global climate change mitigation and demonstrate how equity principles can be applied to differentiate state responsibilities in the context of humanitarian protection. The subsequent analysis of recent efforts to establish effective responsibility-sharing reveals the trade-offs involved in the design of a responsibility allocation mechanism for refugee protection. Our findings provide important lessons for the prospects and limits of responsibility-sharing in asylum governance

Intestinal Tritrichomonas foetus infection in cats in Switzerland detected by in vitro cultivation and PCR

Tritrichomonas foetus, a parasite well known for its significance as venereally transmitted pathogen in cattle, has recently been identified as a cause of chronic large-bowel diarrhea in domestic cats in the US, UK, and, more recently, also in Norway. In a period of 3 months (October to December 2007), 45 cats of Switzerland suffering from chronic diarrhea were investigated for intestinal infections, including a search for trichomonads. A commercially available in vitro culture system was used to screen for infection, complemented with a PCR and subsequent amplicon sequencing to support speciation. The PCR is based upon amplification of a sequence derived from the internal transcribed spacer region 1 (ITS1) on the ribosomal RNA gene (rRNA) using primers designed to detect a broad range of genera and species belonging to the family of Trichomonadidae. The method was furthermore adapted to the uracil DNA glycosylase (UDG) system in order to prevent carry-over contamination and it included a recombinant internal control to track for inhibitory reactions. Eleven out of the 45 cats were culture-positive, as revealed by microscopic identification of trichomonadid organisms. One of the isolates was subjected to scanning electron microscopy and findings revealed the presence of three flagella, thus placing the isolate into the gender Tritrichomonas sp. PCR and subsequent amplicon sequencing were carried out with ten of the 11 isolates. A total homology with published T. foetus sequences was confirmed in all of the cases. T. foetus therefore appears to range among those organisms that can cause chronic diarrhea in cats in Switzerland