Inflammatory and Angiogenic Protein Detection in the Human Vitreous: Cytometric Bead Assay

Introduction. To evaluate clinical feasibility and reproducibility of cytometric bead assay (CBA) in nondiluted vitreous samples of patients with age-related macular degeneration (ARMD), diabetic macular edema (DME), and central retinal vein occlusion (CRVO). Methods. Twelve patients from a single clinics day qualified for intravitreal injections (ARMD n = 6, DME n = 3, CRVO n = 3) and underwent a combination treatment including a single-site 23 gauge core vitrectomy which yielded a volume of 0.6 mL undiluted vitreous per patient. Interleukin-6 (IL-6), vascular endothelial growth factor isoform A (VEGF-A), and monocyte chemo-attractant protein-1 (MCP-1) were assessed directly from 0.3 mL at the same day (fresh samples). To assess the reproducibility 0.3 ml were frozen for 60 days at −80°, on which the CBA was repeated (frozen samples). Results. In the fresh samples IL-6 was highest in CRVO (median IL-6 55.8 pg/mL) > DME (50.6) > ARMD (3.1). Highest VEGF was measured in CRVO (447.4) > DME (3.9) > ARMD (2.0). MCP-1 was highest in CRVO (595.7) > AMD (530.8) > DME (178). The CBA reproducibility after frozen storage was examined to be most accurate for MCP1 (P = 0.91) > VEGF (P = 0.68) > IL-6 (P = 0.49). Conclusions. CBA is an innovative, fast determining, and reliable technology to analyze proteins in fluids, like the undiluted vitreous, which is important to better understand ocular pathophysiology and pharmacology. There is no influence of intermittent storage at −80° for the reproducibility of the CBA

Thermal detector model for cryogenic composite detectors for the dark matter experiments CRESST and EURECA

The CRESST (Cryogenic Rare Event Search with Superconducting Thermometers) and the EURECA (European Underground Rare Event Calorimeter Array) experiments are direct dark matter search experiments where cryogenic detectors are used to detect spin-independent, coherent WIMP (Weakly Interacting Massive Particle)-nucleon scattering events by means of the recoil energy. The cryogenic detectors use a massive single crystal as absorber which is equipped with a TES (transition edge sensor) for signal read-out. They are operated at mK-temperatures. In order to enable a mass production of these detectors, as needed for the EURECA experiment, a so-called composite detector design (CDD) that allows decoupling of the TES fabrication from the optimization procedure of the absorber single-crystal was developed and studied. To further investigate, understand and optimize the performance of composite detectors a detailed thermal detector model which takes into account the CDD has been developed.Comment: To appear in Journal of Physics: Conference Series; Proceedings of Neutrino 2008, Christchurch, New Zealan

Exploring B/Ca as a pH proxy in bivalves : relationships between Mytilus californianus B/Ca and environmental data from the northeast Pacific

© The Author(s), 2011. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Biogeosciences 8 (2011): 2567-2579, doi:10.5194/bg-8-2567-2011.A distinct gap in our ability to understand changes in coastal biology that may be associated with recent ocean acidification is the paucity of directly measured ocean environmental parameters at coastal sites in recent decades. Thus, many researchers have turned to sclerochronological reconstructions of water chemistry to document the historical seawater environment. In this study, we explore the relationships between B/Ca and pH to test the feasibility of B/Ca measured on the ion probe as a pH proxy in the California mussel, Mytilus californianus. Heterogeneity in a range of ion microprobe standards is assessed, leading to reproducible B/Ca ratios at the 5% level. The B/Ca data exhibit large excursions during winter months, which are particularly pronounced during the severe winters of 2004–2005 and 2005–2006. Furthermore, B/Ca ratios are offset in different parts of the skeleton that calcified at the same time. We compare the M. californianus B/Ca record to directly measured environmental data during mussel growth from the period of 1999–2009 to examine whether seawater chemistry or temperature plays a role in controlling shell B/Ca. A suite of growth rate models based on measured temperature are compared to the B/Ca data to optimise the potential fit of B/Ca to pH. Despite sampling conditions that were well-suited to testing a pH control on B/Ca, including a close proximity to an environmental record, a distinct change in pH at the sampling locale, and a growth model designed to optimise the correlations between seawater pH and shell B/Ca, we do not see a strong correlations between pH and shell B/Ca (maximum coefficient of determination, r2, of 0.207). Instead, our data indicate a strong biological control on B/Ca as observed in some other carbonate-forming organisms.Financial support was provided by USGSWHOI Co-operative agreement and NSF-ANT award number 0902957 to L. F. Robinson, a SeaDoc Society grant to C. A. Pfister and J. T. Wootton, and a NASA Planetary Biology Internship to S. J. McCoy with L. F. Robinson and D. M. Glover

Centrifugal force induced by relativistically rotating spheroids and cylinders

Starting from the gravitational potential of a Newtonian spheroidal shell we discuss electrically charged rotating prolate spheroidal shells in the Maxwell theory. In particular we consider two confocal charged shells which rotate oppositely in such a way that there is no magnetic field outside the outer shell. In the Einstein theory we solve the Ernst equations in the region where the long prolate spheroids are almost cylindrical; in equatorial regions the exact Lewis "rotating cylindrical" solution is so derived by a limiting procedure from a spatially bound system. In the second part we analyze two cylindrical shells rotating in opposite directions in such a way that the static Levi-Civita metric is produced outside and no angular momentum flux escapes to infinity. The rotation of the local inertial frames in flat space inside the inner cylinder is thus exhibited without any approximation or interpretational difficulties within this model. A test particle within the inner cylinder kept at rest with respect to axes that do not rotate as seen from infinity experiences a centrifugal force. Although the spacetime there is Minkowskian out to the inner cylinder nevertheless that space has been induced to rotate, so relative to the local inertial frame the particle is traversing a circular orbit.Comment: 12 pages, 2 figure

Hexagons become second if symmetry is broken

Pattern formation on the free surface of a magnetic fluid subjected to a magnetic field is investigated experimentally. By tilting the magnetic field the symmetry can be broken in a controllable manner. When increasing the amplitude of the tilted field, the flat surface gives way to liquid ridges. A further increase results in a hysteretic transition to a pattern of stretched hexagons. The instabilities are detected by means of a linear array of magnetic hall sensors and compared with theoretical predictions.Comment: accepted for publication by Physical Review E/Rapid Communicatio

Quantitative Analysis of Gentamicin Exposure in Neonates and Infants Calls into Question Its Current Dosing Recommendations.

Optimal dosing of gentamicin in neonates is still a matter of debate despite its common use. We identified gentamicin dosing regimens from eight international guidelines and seven Swiss neonatal intensive care units. The dose per administration, the dosing interval, the total daily dose, and the demographic characteristics between guidelines were compared. There was considerable variability with respect to dose (4 to 6 mg/kg), dosing interval (24 h to 48 h), total daily dose (2.5 to 6 mg/kg/day), and patient demographic characteristics that were used to calculate individualized dosing regimens. A model-based simulation study in 1071 neonates was performed to determine the achievement of efficacious peak gentamicin concentrations according to predefined MICs (Cmax/MIC ≥ 10) and safe trough concentrations (Cmin ≤ 2 mg/liter) with recommended dosing regimens. MIC targets of 0.5 and 1 mg/liter were used. Dosing optimization was performed giving priority to the first day of treatment and with the goal of simplifying dosing. Current gentamicin neonatal guidelines allow to achieve effective peak concentrations for MICs ≤ 0.5 mg/liter but not higher. Model-based simulations indicate that to attain peak gentamicin concentrations of ≥10 mg/liter, a dose of 7.5 mg/kg should be administered using an extended dosing interval regimen. Trough concentrations of ≤2 mg/liter can be maintained with a dosing interval of 36 to 48 h in neonates according to gestational and postnatal age. For treatment beyond 3 days, therapeutic drug monitoring is advised to maintain adequate serum concentrations

Rigorous Probabilistic Analysis of Equilibrium Crystal Shapes

The rigorous microscopic theory of equilibrium crystal shapes has made enormous progress during the last decade. We review here the main results which have been obtained, both in two and higher dimensions. In particular, we describe how the phenomenological Wulff and Winterbottom constructions can be derived from the microscopic description provided by the equilibrium statistical mechanics of lattice gases. We focus on the main conceptual issues and describe the central ideas of the existing approaches.Comment: To appear in the March 2000 special issue of Journal of Mathematical Physics on Probabilistic Methods in Statistical Physic