The Role of the Body in Pandemic Geographies of Encounter: Anti-Restriction Protesters Between Collective Action and Political Violence

This article looks at public anti-restriction protests by framing public space as a vital component of urban life. It argues that the body is rarely introduced as a scale of spatial analysis and usually plays a more prominent role in the subfields of social movement or public space studies, which often tend to focus on the transformative and emancipatory side of urban encounters. By integrating a corporeal perspective, the article aims at understanding how the body transforms political passions into individual agency and collective action. Focusing on the Covid-19-crisis-related protests, particularly the anti-restriction protests, the study examines from different angles how a socially heterogeneous group consisting of both radicals and sceptics joined together, in anger, in an atypical coalition concerning state interventions in their very personal spaces. Based on a literature review of secondary sources on anti-restriction protests and an empirical analysis of media coverage of a key event in Vienna, the study identifies a gap in the theorisation of ambivalent geographies of encounter whose impacts range between collective action and political violence. To frame our key hypothesis, considering the body as a scale in spatial analysis is needed for future socio-spatial research to grasp new and pressing urban phenomena of social change. By bridging empirical observation, methodological considerations and conceptual reflection, this article contributes to an understanding of social change through less romanticised modes of analysis of geographies of encounter with a particular take on embodied space

ЛЕВ ПАДАЛКА ЯК ДОСЛІДНИК ЗАПОРОЖЖЯ

Нові публікації останнього часу про формування ідейних та теоретичних засад української історичної науки дають підстави для переконливих висновків про тяглість і цілеспрямованість історіографічного процесу в Україні, а також про роль у ньому як окремих, справді видатних особистостей, так і істориків „другого плану”. Важливе значення для їх становлення мало навчання і залучення до наукової діяльності під егідою і за уважним сприянням фундаторів вітчизняної історичної науки. Переважну більшість серед них склали регіональні школи наукових досліджень, які ввели до наукового обігу величезний комплекс архівних джерел, даних топографії, археології, статистики, етнографії

A Genome-wide screen identifies frequently methylated genes in haematological and epithelial cancers

<p>Abstract</p> <p>Background</p> <p>Genetic as well as epigenetic alterations are a hallmark of both epithelial and haematological malignancies. High throughput screens are required to identify epigenetic markers that can be useful for diagnostic and prognostic purposes across malignancies.</p> <p>Results</p> <p>Here we report for the first time the use of the MIRA assay (methylated CpG island recovery assay) in combination with genome-wide CpG island arrays to identify epigenetic molecular markers in childhood acute lymphoblastic leukemia (ALL) on a genome-wide scale. We identified 30 genes demonstrating methylation frequencies of ≥25% in childhood ALL, nine genes showed significantly different methylation frequencies in B vs T-ALL. For majority of the genes expression could be restored in methylated leukemia lines after treatment with 5-azaDC. Forty-four percent of the genes represent targets of the polycomb complex. In chronic myeloid leukemia (CML) two of the genes, (<it>TFAP2A </it>and <it>EBF2)</it>, demonstrated increased methylation in blast crisis compared to chronic phase (P < 0.05). Furthermore hypermethylation of an autophagy related gene <it>ATG16L2 </it>was associated with poorer prognosis in terms of molecular response to Imatinib treatment. Lastly we demonstrated that ten of these genes were also frequently methylated in common epithelial cancers.</p> <p>Conclusion</p> <p>In summary we have identified a large number of genes showing frequent methylation in childhood ALL, methylation status of two of these genes is associated with advanced disease in CML and methylation status of another gene is associated with prognosis. In addition a subset of these genes may act as epigenetic markers across hematological malignancies as well as common epithelial cancers.</p

Assessing agonistic potential of a candidate therapeutic anti-IL21R antibody

<p>Abstract</p> <p>Background</p> <p>Selective neutralization of the IL21/IL21R signaling pathway is a promising approach for the treatment of a variety of autoimmune diseases. Ab-01 is a human neutralizing anti-IL21R antibody. In order to ensure that the activities of Ab-01 are restricted to neutralization even under <it>in vitro </it>cross-linking and <it>in vivo </it>conditions, a comprehensive assessment of agonistic potential of Ab-01 was undertaken.</p> <p>Methods</p> <p><it>In vitro </it>antibody cross-linking and cell culture protocols reported for studies with a human agonistic antibody, TGN1412, were followed for Ab-01. rhIL21, the agonist ligand of the targeted receptor, and cross-linked anti-CD28 were used as positive controls for signal transduction. <it>In vivo </it>agonistic potential of Ab-01 was assessed by measuring expression levels of cytokine storm-associated and IL21 pathway genes in blood of cynomolgus monkeys before and after IV administration of Ab-01.</p> <p>Results</p> <p>Using a comprehensive set of assays that detected multiple activation signals in the presence of the positive control agonists, <it>in vitro </it>Ab-01-dependent activation was not detected in either PBMCs or the rhIL21-responsive cell line Daudi. Furthermore, no difference in gene expression levels was detected in blood before and after <it>in vivo </it>Ab-01 dosing of cynomolgus monkeys.</p> <p>Conclusions</p> <p>Despite efforts to intentionally force an agonistic signal from Ab-01, none could be detected.</p

Анализ особенностей повышения достоверности кадастровой стоимости

Основной научный результат работы заключается в разработке рекомендаций по повышению достоверности кадастровой стоимости объектов жилой недвижимости, необходимо провести анализ существующей информации данных кадастрового учета, содержащихся в Едином государственном реестре объектов недвижимости.Relevance of the research topic. In connection with the change in the taxation system of residential real estate, municipal authorities and individual owners take an interest in the objectivity and reliability of the results in calculating the cadastral value of real estate objects (further in the text - EO) on thе basis of their actual characteristics. For owners, this is a guarantee of the validity of the base for calculating property tax, or the base for calculating rent for the use of real estate. For local governments, it is regarded as the predictability and financial support in the revenue part of the municipality budget in order to ensure the future socio-economic development of territories. The topic is relevant in terms of designing the development directions and improving the s

Non-Invasive Detection of a Small Number of Bioluminescent Cancer Cells In Vivo

Early detection of tumors can significantly improve the outcome of tumor treatment. One of the most frequently asked questions in cancer imaging is how many cells can be detected non-invasively in a live animal. Although many factors limit such detection, increasing the light emission from cells is one of the most effective ways of overcoming these limitations. Here, we describe development and utilization of a lentiviral vector containing enhanced firefly luciferase (luc2) gene. The resulting single cell clones of the mouse mammary gland tumor (4T1-luc2) showed stable light emission in the range of 10,000 photons/sec/cell. In some cases individual 4T1-luc2 cells inserted under the skin of a nu/nu mouse could be detected non-invasively using a cooled CCD camera in some cases. In addition, we showed that only few cells are needed to develop tumors in these mice and tumor progression can be monitored right after the cells are implanted. Significantly higher luciferase activity in these cells allowed us to detect micrometastases in both, syngeneic Balb/c and nu/nu mice