7,994 research outputs found
Preliminary Study on Treatment of Contaminated Groundwater from the Taylorville Gasifier Site
Groundwater and soil at the site of an abandoned coal gasification plant in Taylorville, Illinois have been contaminated with compounds associated with coal conversion process waters. A preliminary study to assess the feasibility of using ethanol as a means of increasing the solubility of compounds adsorbed within the soil matrix followed by treatment of the ethanol/groundwater extract in an expanded-bed anaerobic granular activated carbon (GAC) reactor was conducted. Results of the study indicate that compounds in the groundwater are highly adsorb able on GAC, and do not interfere with the anaerobic degradation of ethanol in the reactor. Soil extractions with varying ethanol/water ratios were able to remove many additional low water solubility compounds from the soil.ENR Contract Number HWR87035published or submitted for publicationis peer reviewe
Identification of a New Family of Enzymes with Potential \u3cem\u3eO\u3c/em\u3e-acetylpeptidoglycan esterase activity in both Gram-positive and Gram-negative bacteria
Background: The metabolism of the rigid bacterial cell wall heteropolymer peptidoglycan is a dynamic process requiring continuous biosynthesis and maintenance involving the coordination of both lytic and synthetic enzymes. The O-acetylation of peptidoglycan has been proposed to provide one level of control on these activities as this modification inhibits the action of the major endogenous lytic enzymes, the lytic transglycosylases. The O-acetylation of peptidoglycan also inhibits the activity of the lysozymes which serve as the first line of defense of host cells against the invasion of bacterial pathogens. Despite this central importance, there is a dearth of information regarding peptidoglycan O-acetylation and nothing has previously been reported on its de-acetylation.
Results: Homology searches of the genome databases have permitted this first report on the identification of a potential family of O-Acetylpeptidoglycan esterases (Ape). These proteins encoded in the genomes of a variety of both Gram-negative and Gram-positive bacteria, including a number of important human pathogens such as species of Neisseria, Helicobacter, Campylobacter, and Bacillus anthracis, have been organized into three families based on amino acid sequence similarities with family 1 being further divided into three sub-families. The genes encoding these proteins are shown to be clustered with Peptidoglycan O-acetyltransferases (Pat) and in some cases, together with other genes involved in cell wall metabolism. Representative bacteria that encode the Ape proteins were experimentally shown to produce O-acetylated peptidoglycan.
Conclusion: The hypothetical proteins encoded by the pat and ape genes have been organized into families based on sequence similarities. The Pat proteins have sequence similarity to Pseudomonas aeruginosa AlgI, an integral membrane protein known to participate in the O-acetylation of the exopolysaccaride, alginate. As none of the bacteria that harbor the pat genes produce alginate, we propose that the Pat proteins serve to O-acetylate peptidoglycan which is known to be a maturation event occurring in the periplasm. The Ape sequences have amino acid sequence similarity to the CAZy CE 3 carbohydrate esterases, a family previously known to be composed of only O-acetylxylan esterases. They are predicted to contain the α/β hydrolase fold associated with the GDSL and TesA hydrolases and they possess the signature motifs associated with the catalytic residues of the CE3 esterases. Specific signature sequence motifs were identified for the Ape proteins which led to their organization into distinct families. We propose that by expressing both Pat and Ape enzymes, bacteria would be able to obtain a high level of localized control over the degradation of peptidoglycan through the attachment and removal of O-linked acetate. This would facilitate the efficient insertion of pores and flagella, localize spore formation, and control the level of general peptidoglycan turnover
Use of iron salts for control of activated sludge bulking caused by sphaerotilus
A continuing operational problem encountered in the activated sludge system is sludge bulking. While there are several causative agents for this bulking, the filamentous bacterium Sphaerotilus is one of the more common. Control of the growth of this organism will help to eliminate sludge bulking as an operating problem in many activated sludge systems. Iron has been identified in the literature as a possible inhibitor to the growth of this bacterium. However, little is known about the mechanism of this inhibition. This study has shown that the adsorption of iron on Sphaerotilus is the major inhibitory mechanism. The layer of iron on the organism appears to block the transport of nutrients through the sheath and cell wall and hence inhibit the growth of this organism. The effectiveness of the iron compounds in this inhibition corresponds to the physical characteristics of the absorbed iron. Soluble iron complexes form a uniform layer so that the inhibitory effect is proportional to the iron adsorbed. Among the soluble complexes, the ferrous forms are more effective. These forms can penetrate the sheath and deposit on or near the cell wall resulting in greater inhibition. On the other hand, the ferric complexes are deposited on or in the sheath of the organism.U.S. Department of the InteriorU.S. Geological SurveyOpe
The wealth inequality of nations
Comparative research on income inequality has produced several frameworks to study the institutional determinants of income stratification. In contrast, no such framework and much less empirical evidence exist to explain cross-national differences in wealth inequality. This situation is particularly lamentable as cross-national patterns of inequality in wealth diverge sharply from those in income. We seek to pave the way for new explanations of cross-national differences in wealth inequality by tracing them to the influence of different wealth components. Drawing on the literatures on financialization and housing, we argue that housing equity should be the central building block of the comparative analysis of wealth inequality. Using harmonized data on 15 countries included in the Luxembourg Wealth Study (LWS), we demonstrate a lack of association between national levels of income and wealth inequality and concentration. Using decomposition approaches, we then estimate the degree to which national levels of wealth inequality and concentration relate to cross-national differences in wealth portfolios and the distribution of specific asset components. Considering the role of housing equity, financial assets, non-housing real assets, and non-housing debt, we show that cross-national variation in wealth inequality and concentration is centrally determined by the distribution of housing equity
The impact of stripped Nuclei on the Super-Massive Black Hole number density in the local Universe
The recent discovery of super-massive black holes (SMBHs) in the centers of
high-mass ultra compact dwarf galaxies (UCDs) suggests that at least some UCDs
are the stripped nuclear star clusters of lower mass galaxies. Tracing these
former nuclei of stripped galaxies provides a unique way to track the assembly
history of a galaxy or galaxy cluster. In this paper we present a new method to
estimate how many UCDs host an SMBH in their center and thus are stripped
galaxy nuclei. We revisit the dynamical mass measurements that suggest many
UCDs have more mass than expected from stellar population estimates, which
recent observations have shown is due to the presence of an SMBH. We revise the
stellar population mass estimates using a new empirical relation between the
mass-to-light ratio (M/L) and metallicity, and use this to predict which UCDs
are most likely to host an SMBH. This enables us to calculate the fraction of
UCDs that host SMBHs across their entire luminosity range for the first time.
We then apply the SMBH occupation fraction to the observed luminosity function
of UCDs and estimate that in the Fornax and Virgo cluster alone there should be
stripped nuclei with SMBHs. This analysis shows that stripped
nuclei with SMBHs are almost as common in clusters as present-day galaxy
nuclei. We estimate the local SMBH number density in stripped nuclei to
, which represents a significant fraction (10-40\%)
of the SMBH density in the local Universe. These SMBHs hidden in stripped
nuclei will increase expected event rates for tidal disruption events and
SMBH-SMBH and SMBH-BH mergers. The existence of numerous stripped nuclei with
SMBHs are a direct consequence of hierarchical galaxy formation, but until now
their impact on the SMBH density had not been quantified.Comment: 15 pages, 8 Figures, accepted for publication in Ap
Two modes of accelerated glacier sliding related to water
We present the first glacier-wide detailed measurement of basal effective pressure and related observations including bed separation to elucidate the role of water in sliding. The hard bedded glacier instrumented in our study exhibited two phases of accelerated sliding motion apparently driven by separate mechanisms. The first acceleration phase (up to 5 fold increase in speed) was closely tied to an increase in bed separation. The faster second phase (up to 9 fold increase in speed) was related to an unusually high level of connectivity of subglacial waters. We infer the first mode was related to cavity opening and the second mode was related to reduced ice contact with the bed. Glacier sliding over a hard bed is typically represented by sliding laws that include the effective basal pressure, but neither sliding phase was accompanied by a simultaneous decrease in local or regional effective pressure
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