Temas de historia argentina I

Contenidos: - La contratación de aprendices en la actividad artesanal en la ciudad .de Buenos Aires durante el Virreinato. Su análisis a través de los Registros Notariales | Susana Aguirre y Marta Petti - Consideraciones acerca de los matrimonios entre católicos y protestantes en Buenos Aires (1826-1851) | Claudio Panella - Argentina-Brasil: Dinámica de relación en la coyuntura 1914-1918 | Marcelo J. RimoldiFacultad de Humanidades y Ciencias de la Educació

Temas de historia argentina I

Contenidos: - La contratación de aprendices en la actividad artesanal en la ciudad .de Buenos Aires durante el Virreinato. Su análisis a través de los Registros Notariales | Susana Aguirre y Marta Petti - Consideraciones acerca de los matrimonios entre católicos y protestantes en Buenos Aires (1826-1851) | Claudio Panella - Argentina-Brasil: Dinámica de relación en la coyuntura 1914-1918 | Marcelo J. RimoldiFacultad de Humanidades y Ciencias de la Educació

Reestruturação produtiva, qualidade e trabalho : impacto sobre o mercado de trabalho industrial do Estado de São Paulo

Orientador: Maria Carolina de Azevedo F. de SouzaObservações: Falta a página 56.Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Matematica, Estatistica e Computação CientificaResumo: O resumo poderá ser visualizado no texto completo da tese digitalAbstract: The abstract is available with the full electronic digital documentMestradoMestre em Qualidad

Optimization of the bio-functionalized area of magnetic biosensors

In this work, a new method to functionalize a gold surface by dip coating with a functional copolymer is presented. The coating procedure is simple, robust and can be accomplished in less than one hour. Atomic force microscopy (AFM) scratch tests reveal the presence of a homogeneous polymer coating with a thickness of 2.5nm. X-ray photoemission spectroscopy spectra from C1s, N1s and O1s levels present the typical fingerprints of the polymeric overlayer, i.e. the characteristic peaks from CNC   O and NC   O groups. Surface plasmon resonance (SPR) binding assays were used to check the coating functional properties. Immobilization of heparin to SPR gold surfaces functionalized with copoly(DMA-NAS-MAPS)- followed by binding analysis with the well known heparin binding protein fibroblast growth factor 2 yield binding kinetic parameters comparable to those obtained with commercially available carboxymethyl dextran- functionalized sensorchips, thus confirming the great potential of the proposed technique

Identification of DNA Rearrangements at the Retinoic Acid Receptor-a (RAR-a) Locus in all Patients With Acute Promyelocytic Leukemia (APL) and mapping of APL Breakpoints within the RAR-a second intron

Seventy patients with acute promyelocytic leukemia (APL) were characterized at the DNA level using genomic retinoic acid receptor-alpha (RAR-alpha) probes on Southern blot experiments. Sixty-two cases were defined as M3 according to the French-American-British (FAB) criteria, and eight had a diagnosis of microgranular or variant (M3v) APL. The use of two restriction enzymes and three probes exploring the second intron of the RAR-alpha gene allowed us to detect specific abnormal DNA fragments in every case, with clustering of rearrangements within the 20-kb intronic region between RAR-alpha exons II and III. A more detailed mapping of APL breakpoints was performed in 52 cases in which three EcoRI subregions of the RAR-alpha second intron were analyzed with corresponding probes. Comparison of clinical and hematological features in the three subgroups of patients with distinct RAR-alpha breakpoints did not show significant differences regarding age, peripheral blood (PB) counts, presence of coagulopathy, or FAB classification (M3 v M3v). Interestingly, a significant difference was observed in the M/F ratio of the three subgroups, with a higher incidence of rearrangements at the 5' end of the RAR-alpha second intron in female patients, and more frequent 3' breakpoints in males. The results of this study indicate that a unique genomic alteration consistently occurs on the 17q- derivative of the APL specific t(15;17) aberration. Moreover, the clinical relevance of RAR-alpha gene analysis both at diagnosis and in follow-up studies is further emphasized

Human Papillomaviruses and the Interferon Response

Human papillomaviruses (HPV) are small DNA viruses that target stratified keratinocytes for infection. A subset of HPV types infect epithelia in the genital tract and are the causative agents of cervical as well as other anogenital cancers. Interferon treatment of existing genital HPV lesions has had mixed results. While HPV proteins down-regulate the expression of interferon-inducible genes, interferon treatment ultimately induces their high-level transcription after a delay. Cells containing complete HPV genomes that are able to undergo productive replication upon differentiation are sensitive to interferon-induced growth arrest, while cells from high-grade cancers that only express E6 and E7 are resistant. Recent studies indicate this sensitivity is dependent upon the binding of the interferon-inducible factor, p56, to the E1 replication protein. The response to interferon by HPV proteins is complex and results from the action of multiple viral proteins