Crossed-beam universal-detection reactive scattering of radical beams characterized by laser-induced-fluorescence: the case of C2 and CN

International audienceWe have generated continuous supersonic beams of dicarbon (C2) and cyano (CN) radicals by a high-pressure radio-frequency discharge beam source starting from dilute mixtures in rare gases of suitable precursor molecules. We have subsequently characterized their internal quantum state distributions by laser-induced-fluorescence (LIF) in a new crossed molecular beam-laser apparatus. We have used these supersonic beams to study the reactive scattering of C2 and CN radicals with unsaturated hydrocarbons. We report here on the C2 and CN radical beam characterization by LIF and on dynamics studies of the reactions CN + C2H2 (acetylene) and CN + CH3CCH (methylacetylene) by the crossed molecular beam scattering technique with universal mass spectrometric detection and time-of-flight analysis. The role of CN rovibrational excitation on the dynamics of the CN + C2H2 reaction is discussed with reference to previous dynamics and kinetics studies. These reactions are of interest in the chemistry of planetary atmospheres (Titan) and the interstellar medium as well as in combustion

Monitoring of people and workers exposure to the electric, magnetic and electromagnetic fields in an Italian national cancer Institute

<p>Abstract</p> <p>Background</p> <p>The paper reports the electric, magnetic and electromagnetic fields (<it>emf</it>) measurements carried out in the <it>Regina Elena National Cancer Institute (NCI)</it>. Several devices, used in diagnostics and in medical cures, can represent sources of <it>emf </it>for the workers and for the public subjected to the treatments. The aim is to evaluate their exposition, in order to assess the compliance with the law.</p> <p>Methods</p> <p>The investigations have been carried out in the departments of: intensive care, physiotherapy, MR presstherapy and in the surgical rooms. The measurements have been performed using broad band probes in the frequency ranges 5 Hz÷30 kHz and 100 kHz-3 GHz.</p> <p>Results</p> <p>The variability of the magnetic induction (B(μT)) levels is between 0,05 μT and 80 μT. The statistical distribution shows that most of the measurements are in the range 0,05<B = 0,5 μT and the 89% of the B(μT) levels are within the 3 μT.</p> <p>Conclusion</p> <p>The measurement of the <it>emf </it>levels in the <it>NCI </it>is recommended because of the presence of the oncological patients; their long stay near the equipments and their day-long exposure represent additional risk factors for which a prudent avoidance strategy have to de adopted.</p

Elotuzumab plus pomalidomide and dexamethasone in relapsed/refractory multiple myeloma: a multicenter, retrospective real-world experience with 200 cases outside of controlled clinical trials

In the ELOQUENT-3 trial, the combination of elotuzumab, pomalidomide and dexamethasone (EloPd) proved a superior clinical benefit over Pd with a manageable toxicity profile, leading to its approval in relapsed/refractory multiple myeloma (RRMM), who had received at least two prior therapies, including lenalidomide and a proteasome inhibitor (PI). We report here a real-world experience of 200 RRMMs treated with EloPd in 35 Italian centers outside of clinical trials. In our dataset, the median number of prior lines of therapy was 2, with 51% of cases undergoing autologous stem cell transplant (ASCT) and 73% exposed to daratumumab. After a median follow-up of 9 months, 126 patients stopped EloPd, most of them (88.9%) because of disease progression. The overall response rate (ORR) was 55.4%, in line with the pivotal trial results. Regarding adverse events, our cohort experienced a toxicity profile similar to the ELOQUENT-3 trial, with no significant differences between younger (&lt;70 years) and older patients. The median progression-free survival (PFS) was 7 months, shorter than that observed in the ELOQUENT-3, probably due to the different clinical characteristics of the two cohorts. Interestingly, the ISS stage III (HR:2.55) was associated with worse PFS. Finally, our series's median overall survival (OS) was shorter than that observed in the ELOQUENT-3 trial (17.5 versus 29.8 months). In conclusion, our real-world study confirms EloPd as a safe and possible therapeutic choice for RRMM who received at least two prior therapies, including lenalidomide and a PI

Association of Variants in the SPTLC1 Gene With Juvenile Amyotrophic Lateral Sclerosis

Importance: Juvenile amyotrophic lateral sclerosis (ALS) is a rare form of ALS characterized by age of symptom onset less than 25 years and a variable presentation.Objective: To identify the genetic variants associated with juvenile ALS.Design, Setting, and Participants: In this multicenter family-based genetic study, trio whole-exome sequencing was performed to identify the disease-associated gene in a case series of unrelated patients diagnosed with juvenile ALS and severe growth retardation. The patients and their family members were enrolled at academic hospitals and a government research facility between March 1, 2016, and March 13, 2020, and were observed until October 1, 2020. Whole-exome sequencing was also performed in a series of patients with juvenile ALS. A total of 66 patients with juvenile ALS and 6258 adult patients with ALS participated in the study. Patients were selected for the study based on their diagnosis, and all eligible participants were enrolled in the study. None of the participants had a family history of neurological disorders, suggesting de novo variants as the underlying genetic mechanism.Main Outcomes and Measures: De novo variants present only in the index case and not in unaffected family members.Results: Trio whole-exome sequencing was performed in 3 patients diagnosed with juvenile ALS and their parents. An additional 63 patients with juvenile ALS and 6258 adult patients with ALS were subsequently screened for variants in the SPTLC1 gene. De novo variants in SPTLC1 (p.Ala20Ser in 2 patients and p.Ser331Tyr in 1 patient) were identified in 3 unrelated patients diagnosed with juvenile ALS and failure to thrive. A fourth variant (p.Leu39del) was identified in a patient with juvenile ALS where parental DNA was unavailable. Variants in this gene have been previously shown to be associated with autosomal-dominant hereditary sensory autonomic neuropathy, type 1A, by disrupting an essential enzyme complex in the sphingolipid synthesis pathway.Conclusions and Relevance: These data broaden the phenotype associated with SPTLC1 and suggest that patients presenting with juvenile ALS should be screened for variants in this gene.</p

The earliest Middle Pleistocene Crocuta crocuta (Erxleben, 1777) at Casal Selce (Rome, Italy)

Hyaenas are relevant members of the Plio-Pleistocene carnivore guilds in Africa and Eurasia. The spotted hyena Crocuta crocuta is an important element of the dispersal events from Africa and Asia to Europe occurred at the Early-Middle Pleistocene transition (about 0.8 Ma). The presence of Crocuta crocuta remains from the Ponte Galeria formation at Casal Selce (Roma, Italy) from sands of the Ponte Galeria Formation referable to the earliest Middle Pleistocene (MIS 18), close to the Brunhes/Matuyama boundary, is one of the earliest occurrence of the genus in Europe. (C) 2012 Elsevier Ltd and INQUA. All rights reserved

Ursus etruscus Cuvier, 1823 from the Early Pleistocene of Monte Argentario (Southern Tuscany, Central Italy).

Ursus etruscus fossils have been recovered in the Monte Argentario site (late Villafranchian, Early Pleistocene, Tuscany). Teeth and bones show the typical morphology of the Etruscan bear. The occurrence of this species in the Early Pleistocene Monte Argentario faunal assemblage and other coeval sites (i.e. Pirro Nord) is very important in the framework of the Early Pleistocene biochronology. The occurrence of Etruscan bear at Monte Argentario and Pirro Nord confirms that Ursus etruscus persists in the Early Pleistocene assemblages, representing therefore an European carnivore coexisting with new taxa dispersing from Africa and Asia

The Middle-Late Pleistocene Cuon Hodgson, 1838 (Carnivora, Canidae) from Italy

The aim of this work is to review the Middle-Late Pleistocene fossil remains of the genus Cuon Hodgson, 1838 from Italy. The geographical distribution of hypercarnivomus canids belonging to the genus Cuon is presently restricted to Southeastern Asia, whereas during the Pleistocene occurrences are documented in Eurasia and North America. Due to the fragmentary nature of the fossil record of this genus, resolution of many aspects of its origins, phyletic relationships, and evolutionary trends prove difficult. In the Italian Peninsula the occurrence of thy canid has been reported from several sites of Middle-Late Pleistocene age. However; the latter have rarely been accompanied by descriptions oldie material. This paper presents an update of the Italian record of the genus Cuon, description of the material from these different sites and includes unpublished data related to new localities. It represents a starting point for a broader review of the Mediterranean Plio-Pleistocene hypercarnivorous canid

The African species Megantereon whitei from the Early Pleistocene of Monte Argentario (South Tuscany, Central Italy)

A partial skull and articulated postcranial elements of Megantereon whitei have been recorded during the 1950s from a karst deposit in the Monte Argentario area (Grosseto). These fossils recently became available for study. The bones are quite well preserved, included in a hard reddish matrix with calcareous clasts. The fossil is part of a faunal assemblage referred to the Late Villafranchian (Early Pleistocene). M. whitei from Monte Argentario is characterised by elongated upper canines, stronger than those of the Upper Valdarno (Tuscany) specimens and similar to the Pirro Nord (Apulia) fossil. The manus is robust, the first phalanges are quite long in comparison to the metacarpals. The Early Pleistocene European M. whitei represents an African element that took part in the faunal dispersal from Africa to Europe that occurred at the Plio-Pleistocene transition

L'innovazione digitale attraverso il networking nel settore agroalimentare

Scopo del paper è di comprendere il ruolo dei Progetti Europei di Ricerca e Sviluppo (ERDP) nei processi di innovazione digitale delle piccole e medie imprese agro-alimentari