Conservation Reserve Program research and demonstration project

This project, the first of its kind in the nation, used land enrolled in the Conservation Reserve Program to demonstrate intensive rotational grazing as an economically and environmentally viable alternative use for highly erodible land due to come out of CRP contracts beginning in 1996. A local committee secured funds and expertise from various agencies to establish three grazing systems. Forage improvement, innovative watering and fencing systems, contour lanes, and other aspects of grazing were implemented and evaluated. The project also included a field survey inventory of land in 300 CRP contracts in a three-county area. Another important segment of this five-year project involved education activities such as field days, grazing clinics, tours, and presentations both on and off the site

Education-demonstration for intensive grazing and forage management on highly erodible land

The wise use of highly erodible marginal land is an important economic and environmental issue. Rotational grazing for livestock is one method of land use improvement suitable for HEL or other cropland that needs to be converted to pasture. Using an existing demonstration farm in Adams County, Iowa, various paddock arrangements and forage varieties were tested to help farmers devise appropriate sustainable grazing systems

Allosteric Activation of the Par-6 PDZ Via a Partial Unfolding Transition

Proteins exist in a delicate balance between the native and unfolded states, where thermodynamic stability may be sacrificed to attain the flexibility required for efficient catalysis, binding, or allosteric control. Partition-defective 6 (Par-6) regulates the Par polarity complex by transmitting a GTPase signal through the Cdc42/Rac interaction binding PSD-95/Dlg/ZO-1 (CRIB-PDZ) module that alters PDZ ligand binding. Allosteric activation of the PDZ is achieved by local rearrangement of the L164 and K165 side chains to stabilize the interdomain CRIB:PDZ interface and reposition a conserved element of the ligand binding pocket. However, microsecond to millisecond dynamics measurements revealed that L164/K165 exchange requires a larger rearrangement than expected. The margin of thermodynamic stability for the PDZ domain is modest (∼3 kcal/mol) and further reduced by transient interactions with the disordered CRIB domain. Measurements of local structural stability revealed that tertiary contacts within the PDZ are disrupted by a partial unfolding transition that enables interconversion of the L/K switch. The unexpected participation of partial PDZ unfolding in the allosteric mechanism of Par-6 suggests that native-state unfolding may be essential for the function of other marginally stable proteins

Genetic protocols for DNA extraction from white-tailed deer cast antlers to confirm individuality

White-tailed deer (Odocoileus virginianus) are the most sought-after deer species in America. The antlers of mammals, such as deer, are one of the fastest regenerative tissues in the world and are grown and naturally cast every year. Research on cast antlers have been used for a variety of purposes including population comparisons and impacts of deer health due to climatic stressors. When investigating cast antlers, it is important to confirm individuality of match sets in addition to antlers of the same individual between years. Therefore, individuality must be confirmed genetically, and protocols must be developed and established to do so. Our objectives were to 1) establish a genetic protocol to harvest DNA from cast antlers using connective tissue, and 2) determine individuality from subsequent years and match sets. When fresh antlers are cast, they leave behind a viable connective tissue from which DNA can be extracted. The DNA was successfully extracted from the skin rings harvested from naturally cast antlers. This study developed viable methods to confirm individuality, which aid researchers and wildlife biologists in a better understanding of the white-tailed deer herd as they set management goals and harvest regulations

A Systems Engineering Approach to Aircraft Kinetic Kill Countermeasures Technology: Development of an Active Aircraft Defense System for the C/KC-135 Aircraft

Modern Surface to Air Missiles (SAMs) present a significant threat to today\u27s military and civilian aircraft. Current countermeasure systems such as flares and chaff rely on decoying the missile threat and do not provide adequate protection against advanced computerized missiles (Schaffer, 1993:1). An aircraft defense system that actively seeks out and defeats an incoming missile by placing a physical barrier in the missile\u27s path offers a promising alternative to current countermeasures technology. This thesis reports the preliminary design of an active aircraft defense system for the protection of the C/KC-135 aircraft from SAMs. The developed system utilizes a kinetic kill mechanism to protect the aircraft from shoulder launched missiles while the aircraft is in the takeoff and climb-out configurations. Both smart anti-missile expendables and dumb projectile expendables are evaluated. The iterative Systems Engineering approach is used to narrow the solution set to the optimal design. The final outcome is the refined design of two candidate aircraft defense system employing a kinetic kill mechanism. Both systems utilize a modified ultra-violet tracker and employ one of two types of nets, one made out of Detonation Cord and the other made out of Spectra

Policy Brief #1: Child Care Assets: What are 14 Key Assets of Child Care Providers that Support Quality?

In 2000, university researchers at the University of Nebraska-Lincoln, Iowa State University, University of Kansas and the University of Missouri and state child care and early education program partners in four states (Missouri, Iowa, Kansas, and Nebraska) initiated the Midwest Child Care Research Consortium (MCCRC). The focus of the Consortium’s work is to conduct a multiyear study on a range of issues associated with child care quality and conditions. Across the four states, a stratified random selection of 2,022 child care providers participated in a telephone survey conducted by the Gallup Organization, representing licensed child care centers, licensed family child care homes, registered child care homes, and subsidized care license exempt family and (in one state) license exempt center care. Providers responded to questions about background and practices often associated with quality. Of the providers responding to the phone survey, 365 were randomly selected for in-depth observations to assess quality, using conventional measures of child care quality (see back of this brief). This report shows the relation between observed quality and many provider characteristics and professional improvement efforts

Nebraska Child Care Workforce and Quality: Summary Policy Brief #7

The study showed the average child care provider in Nebraska is female, married and a parent. This provider had some training or education beyond high school but not an advanced degree, was active in child care training, had a First Aid/CPR certificate, considered child care her profession or calling, had been in the child care field for over 5 years and planned to remain a provider. The average provider was observed to provide minimal quality child care. In Nebraska, using well-established observational measures of quality, center-based preschool care averaged 4.16 on the Early Childhood Environment Rating Scale (ECERS-R); 4.49 on the Infant Toddler Environment Rating Scale (ITERS); and family child care averaged 4.46 on the Family Day Care Rating Scale (FDCRS). A “5” is considered “good” quality. There was great variability across all types of care. · Family child care quality was higher in Nebraska and Missouri than in Iowa and Kansas. · In center-based care, there were no differences between providers who cared for children receiving government child care subsidies and those who did not but in family child care there were differences. Quality, training, education and professionally-oriented attitudes were lower among subsidy-receiving family child care providers than for non-subsidy receiving counterparts. · Providers in Early Head Start/Head Start partnerships offered higher quality care and received more training than other child care providers. Nebraska like two other states invested training funds to enable Early Head Start/Head Start programs to partner with programs to follow the Head Start Performance Standards and these partnerships did appear to result in higher quality than average