229 research outputs found
Supplementary data for article: DodoÅ”, T.; RajÄeviÄ, N.; TeÅ”eviÄ, V.; Marin, P. D. Chemodiversity of Epicuticular N-Alkanes and Morphological Traits of Natural Populations of Satureja Subspicata Bartl. Ex Vis. along Dinaric Alps ā Ecological and Taxonomic Aspects. Chemistry and Biodiversity 2017, 14 (2). https://doi.org/10.1002/cbdv.201600201
Supplementary material for: [https://doi.org/10.1002/cbdv.201600201]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2416
Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data
Pinus nigra J.F. Arnold, European black pine, is a typical component of
Mediterranean and sub-Mediterranean coniferous forests with highly
fragmentary distribution. Western Mediterranean populations of this
species have been studied genetically to date, while eastern populations
from the central Balkans, which are larger and more abundant, are still
genetically understudied. We analyzed seven populations of P. nigra
representing all infraspecific taxa recognized within the central
Balkans (subspecies nigra with varieties nigra and gocensis Dordevic;
and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and
banatica (Endl.) Georgescu et Ionescu), with three chloroplast
microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus.
Although our molecular data failed to support circumscription of studied
infraspecific taxa, we found that genetic patterns at both genomes are
in accordance with those found previously in westward populations of
this species, that is - exceptionally high levels of genetic diversity
(H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the
cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and
genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on
genealogical relations of mtDNA types currently present in Balkans' and
Iberian/African populations, we inferred that the ancestral gene pool of
P. nigra already harbored polymorphism at position 328 prior to the
divergence to two lineages currently present in westward and eastward
parts of the species range distribution. Subsequent occurrence of three
mutations, which distinguish these two lineages, suggests their
long-term isolation.Ministry of Education, Science, and Technology of the Republic of Serbia
{[}173029, 173030
Supplementary data for the article: NikoliÄ, B.; MatoviÄ, M.; TodosijeviÄ, M.; StankoviÄ, J.; CvetkoviÄ, M.; Marin, P. D.; TeÅ”eviÄ, V. Volatiles of Tanacetum Macrophyllum Obtained by Different Extraction Methods. Natural Product Communications 2018, 13 (7), 891ā893. https://doi.org/10.1177/1934578x1801300727
Supplementary material: [https://doi.org/10.1177/1934578X1801300727]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2169
n-alkanes in the needle waxes of Pinus heldreichii var. panÄiÄi
This is the first report of n-alkanes in needle epicuticular waxes of the variety Bosnian pine, Pinus heldreichii var. panÄiÄi. n-Hexane extracts of needle samples, originating from seven isolated localities in Serbia, were analysed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The results evidenced n-alkanes ranging from C18 to C33 in epicuticular waxes. The most abundant alkanes were C27, C23, C25 and C29 (12.53 %, 12.46 %, 12.00 % and 10.38 % on average, respectively). The carbon preference index (CPItotal) of Pinus heldreichii var. panÄiÄi ranged from 1.1 to 2.1 (1.6 on average), while the average chain length (ACLtotal) ranged from 25.0 to 25.8 (25.3 on average). A high level of individual quantitative variation in all of these hydrocarbon parameters was also found. The obtained results were compared with the bibliographic references for Pinus heldreichii var. leucodermis and other species of the Pinus genus.Ovo je prvo saopÅ”tenje o n-alkanima u voskovima iglica varijeteta munike, Pinus heldreichii var. panÄiÄi. n-Heksanski ekstrakti uzoraka iglica koji potiÄu sa sedam izolovanih lokaliteta u Srbiji analizirani su gasnom hromatografijom (GC) i gasnom hromatografijom-masenom spektrometrijom (GC-MS). Rezultati su pokazali da se n-alkani nalaze u opsegu od C18 do C33. MeÄu njima su najobilniji C27, C23, C25 i C29 (12,53, 12,46, 12,00 i 10,38 % u proseku, redom). UgljeniÄni preferencijalni indeks (CPItotal) Pinus heldreichii var. panÄiÄi je bio u opsegu od 1,1 do 2,1 (proseÄno 1,6), a dužina niza ugljenikovih atoma (ACLtotal) u opsegu od 25,0 do 25,8 (proseÄno 25,3). TakoÄe je utvrÄen visok nivo individualne kvantitativne varijabilnosti u svim analiziranim parametrima ovih ugljovodonika. Dobijeni rezultati su uporeÄeni sa literaturnim podacima koji se odnose na Pinus heldreichii var. leucodermis i druge vrste roda Pinus.Projekat ministarstv
Population diversity of n-alkanes in the needle cuticular wax of relicts pinus heldreichii and P. peuce from the scardo-pindic mountains
The variability of n-alkanes in the needle cuticular wax of Pinus heldreichii and P. peuce in two natural populations from the Scardo-Pindic mountains was investigated for the first time. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS) analyses of two-year-old needles were performed us-ing an Agilent 7890A GC equipped with an inert 5975C XL EI/CI mass spectrometer detector (MSD) and a flame ionization detector (FID) connected by a capillary flow technology 2-way splitter with make-up. An HP-5MS capillary column was used. n-Alkanes ranged from C19 to C33. In P. heldreichii the most abundant were C23, C25 and C27 (16.3, 15.6 and 12.8 % on average, respectively), while in P. peuce they were C29, C25, and C27 (16.5, 15.3 and 13.5 % on average, resp.). Mid-chain and long-chain n-alkanes prevailed in both species. Principle component analysis (PCA) and Cluster analyses of five and six n-alkanes, respectively, showed divergence of the Scardo-Pindic populations from the Dinaric ones
Supplementary data for article: DodoÅ”, T.; RajÄeviÄ, N.; TeÅ”eviÄ, V.; Marin, P. D. Chemodiversity of Epicuticular N-Alkanes and Morphological Traits of Natural Populations of Satureja Subspicata Bartl. Ex Vis. along Dinaric Alps ā Ecological and Taxonomic Aspects. Chemistry and Biodiversity 2017, 14 (2). https://doi.org/10.1002/cbdv.201600201
Supplementary material for: [https://doi.org/10.1002/cbdv.201600201]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2416
n-alkani u voskovima iglica Pinus heldreichii var. panÄiÄi
This is the first report of n-alkanes in needle epicuticular waxes of the variety Bosnian pine, Pinus heldreichii var. pancici. n-Hexane extracts of needle samples, originating from seven isolated localities in Serbia, were analysed by gas chromatography (GC) and gas chromatography mass spectrometry (GC MS). The results evidenced n-alkanes ranging from C(18) to C(33) in epicuticular waxes. The most abundant alkanes were C(27), C(23), C(25) and C(29) (12.53 %, 12.46 %, 12.00 % and 10.38 % on average, respectively). The carbon preference index (CPI(total)) of Pinus heldreichii var. pancici ranged from 1.1 to 2.1 (1.6 on average), while the average chain length (ACL(total)) ranged from 25.0 to 25.8 (25.3 on average). A high level of individual quantitative variation An all of these hydrocarbon parameters was also found. The obtained results were compared with the bibliographic references for Pinus heldreichii var. leucodermis and other species of the Pinus genus.Ovo je prvo saopÅ”tenje o n-alkanima u voskovima iglica varijeteta munike, Pinus heldreichii var. panÄiÄi. n-Heksanski ekstrakti uzoraka iglica koji potiÄu sa sedam izolovanih lokaliteta u Srbiji analizirani su gasnom hromatografijom (GC) i gasnom hromatografijom-masenom spektrometrijom (GC-MS). Rezultati su pokazali da se n-alkani nalaze u opsegu od C18 do C33. MeÄu njima su najobilniji C27, C23, C25 i C29 (12,53, 12,46, 12,00 i 10,38 % u proseku, redom). UgljeniÄni preferencijalni indeks (CPItotal) Pinus heldreichii var. panÄiÄi je bio u opsegu od 1,1 do 2,1 (proseÄno 1,6), a dužina niza ugljenikovih atoma (ACLtotal) u opsegu od 25,0 do 25,8 (proseÄno 25,3). TakoÄe je utvrÄen visok nivo individualne kvantitativne varijabilnosti u svim analiziranim parametrima ovih ugljovodonika. Dobijeni rezultati su uporeÄeni sa literaturnim podacima koji se odnose na Pinus heldreichii var. leucodermis i druge vrste roda Pinus
Nonacosan-10-ol and n-Alkanes in Leaves of Pinus pinaster
In leaf cuticular wax of Pinus pinaster, content of nonacosan-10-ol is high (77.1% on average). n-Alkanes ranged from C18 to C35 with the most dominant C29 (24.8%). The carbon preference index (CPItotal) ranged from 3.1 to 5.6 (4.0 on average), while the average chain length (ACLtotal) ranged from 14.0 to 17.0 (14.8 on average). Long-chain n-alkanes (n-C25-35) strongly dominated (80.1%) over middle-chain (n-C21-24= 18.9%) and short-chain (n-C18-20= 0.9%) n-alkanes
Composition and antimicrobial activity of essential oils of artemisia judaica, a. Herba-alba and a. Arborescens from Libya
The essential oils obtained by hydrodistillation from the aerial parts of Artemisia judaica L., Artemisia herbaalba Asso. and Artemisia arborescens L. (cultivated) from Libya, were analyzed by GC and GC-MS. The antimicrobial properties were determined using the broth microdilution method against eight bacterial species: Bacillus cereus (clinical isolate), Micrococcus flavus (ATCC10240), Listeria monocytogenes (NCTC7973), Staphylococcus aureus (ATCC6538), Escherichia coli (ATCC35210), Pseudomonas aeruginosa (ATCC27853), Salmonella typhimurium (ATCC13311), Enterobacter cloacae (human isolates) and eight fungal species: Aspergillus niger (ATCC6275), A. ochraceus (ATCC12066), A. versicolor (ATCC11730), A. fumigatus (ATCC1022), Penicillium ochrochloron (ATCC9112), P. funiculosum (ATCC10509), Trichoderma viride (IAM5061) and Candida albicans (human isolate). The major constituents of A. arborescens oil were sesquiterpene hydrocarbons (47.4%). Oxygenated monoterpenes were the dominant constituents in the A. judaica and A. herba-alba oils (54.2% and 77.3%, respectively). Camphor (24.7%) and chamazulene (20.9%) were the major components in the essential oil of A. arborescens, chrysanthenone (20.8%), cis-chrysanthenyl acetate (17.6%) and cis-thujone (13.6%) dominated in the A. herba-alba oil, and the major constituents in the A. judaica oil were piperitone (30.21%) and cis-chrysanthenol (9.1%). The best antimicrobial activity was obtained for A. judaica oil and the lowest effect was noticed in A. arborescens oil. The effect of the tested oils was higher against Gram (+) than Gram (-) bacteria. All three oils showed the best antibacterial activity against Listeria monocytogenes and the lowest against Pseudomonas aeruginosa, Escherichia coli, Enterobacter cloacae, compared to streptomycin and ampicillin. All three oils showed better antifungal activities than ketoconazole, except A. arborescens oil against Aspergillus niger
Composition and antimicrobial activity of the essential oil from Galatella linosyris (L.) Rchb. f. (Asteraceae)
An investigation of the chemical composition and antimicrobial activity of the essential oil of Galatella linosyris is presented. The chemical analysis (GC/MS, NMR) showed that sabinene (40 %), Ī²-pinene (35.5 %), Ī±-pinene (4.5 %), limonene (4 %), Ī³-muurolene (4 %), and (E)-caryophyllene (3.3 %) were dominant components in this oil. Microdilution assays were used to evaluate the minimum inhibitory concentration (MIC) and the minimum bactericidal/ fungicidal concentrations (MBC/MFC). G. linosyris essential oil exhibited better antibacterial activity against some of the tested bacteria than antifungal activity.U ovom radu je prikazano ispitivanje hemijskog sastava i antimikrobne aktivnosti etarskog ulja biljne vrste Galatella linosyris. Hemijskom analizom (GC-MS i NMR) je utvrÄeno da su glavni sastojci ovog etarskog ulja sabinen (40 %), Ī²-pinen (35,5 %) , Ī±-pinen (4,5 %), limonen (4 %), Ī³-muurolen (4 %) i (E)-kariofilen (3,3 %). Za procenu minimalne inhibitorne koncentracije (MIC) i minimalne baktericidne/fungicidne koncentracije (MBC/ /MFC) koriÅ”Äeni su testovi mikrorazblaženja. Etarsko ulje G. linosyris je pokazalo bolju antibakterijsku nego antifungalnu aktivnost.Projekat ministarstva br. 173032, br. 173029 and br. 17205
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