1,921 research outputs found

    Preparation of a chelating sorbent based on pyridylethylated polyethylenimine for recovering transition metal ions

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    A method was developed for preparing a chelating amino polymer, pyridylethylpolyethylenimine with maximal degree of substitution, by polymer-analogous transformations of branched polyethylenimine in reaction with 2-vinylpyridine. The ability of cross-linked pyridylethylpolyethylenimine with the degree of substitution of 0.32 to sorb Cu2+, Ni2+, Co2+, Zn2+, Cd2+, Mn2+, and Pb 2+ ions present simultaneously in solution was evaluated. In an ammonium acetate sorption system (pH 3.5-4.0), the sorbent selectively interacts with Cu(II) ions. © 2013 Pleiades Publishing, Ltd

    Evolutionary diversification of the BetaM interactome acquired through co-option of the ATP1B4 gene in placental mammals

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    ATP1B4 genes represent a rare instance of orthologous vertebrate gene co-option that radically changed properties of the encoded BetaM proteins, which function as Na, K-ATPase subunits in lower vertebrates and birds. Eutherian BetaM has lost its ancestral function and became a muscle-specific resident of the inner nuclear membrane. Our earlier work implicated BetaM in regulation of gene expression through direct interaction with the transcriptional co-regulator SKIP. To gain insight into evolution of BetaM interactome we performed expanded screening of eutherian and avian cDNA libraries using yeast-two-hybrid and split-ubiquitin systems. The inventory of identified BetaM interactors includes lamina-associated protein LAP-1, myocyte nuclear envelope protein Syne1, BetaM itself, heme oxidases HMOX1 and HMOX2; transcription factor LZIP/CREB3, ERGIC3, PHF3, reticulocalbin-3, and beta-sarcoglycan. No new interactions were found for chicken BetaM and human Na, K-ATPase beta 1, beta 2 and beta 3 isoforms, indicating the uniqueness of eutherian BetaM interactome. Analysis of truncated forms of BetaM indicates that residues 72-98 adjacent to the membrane in nucleoplasmic domain are important for the interaction with SKIP. These findings demonstrate that evolutionary alterations in structural and functional properties of eutherian BetaM proteins are associated with the increase in its interactome complexity

    Measurement of the e+eK+Kπ+πe^+e^- \to K^+K^-\pi^+\pi^- cross section with the CMD-3 detector at the VEPP-2000 collider

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    The process e+eK+Kπ+πe^+e^- \to K^+K^-\pi^+\pi^- has been studied in the center-of-mass energy range from 1500 to 2000\,MeV using a data sample of 23 pb1^{-1} collected with the CMD-3 detector at the VEPP-2000 e+ee^+e^- collider. Using about 24000 selected events, the e+eK+Kπ+πe^+e^- \to K^+K^-\pi^+\pi^- cross section has been measured with a systematic uncertainty decreasing from 11.7\% at 1500-1600\,MeV to 6.1\% above 1800\,MeV. A preliminary study of K+Kπ+πK^+K^-\pi^+\pi^- production dynamics has been performed

    Study of the process e+eppˉe^+e^-\to p\bar{p} in the c.m. energy range from threshold to 2 GeV with the CMD-3 detector

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    Using a data sample of 6.8 pb1^{-1} collected with the CMD-3 detector at the VEPP-2000 e+ee^+e^- collider we select about 2700 events of the e+eppˉe^+e^- \to p\bar{p} process and measure its cross section at 12 energy ponts with about 6\% systematic uncertainty. From the angular distribution of produced nucleons we obtain the ratio GE/GM=1.49±0.23±0.30|G_{E}/G_{M}| = 1.49 \pm 0.23 \pm 0.30

    Application of AFM for the investigation the roughness of imaging optics elements

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    Обсуждается специфика применения атомно-силовой микроскопии (АСМ) для измерения шероховатости изображающих оптических элементов. Описывается стенд на основе АСМ для измерения образцов сложной формы с большими латеральными размерами и методика измерений.The features of the application of atomic force microscopy for measuring the roughness of imaging optical elements are discussed. A stand on the basis of AFM for measuring samples of complex shape with large lateral dimensions and a measurement method is described

    Non-isothermal model for the direct isotropic/smectic-A liquid crystalline transition

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    An extension to a high-order model for the direct isotropic/smectic-A liquid crystalline phase transition was derived to take into account thermal effects including anisotropic thermal diffusion and latent heat of phase-ordering. Multi-scale multi-transport simulations of the non-isothermal model were compared to isothermal simulation, showing that the presented model extension corrects the standard Landau-de Gennes prediction from constant growth to diffusion-limited growth, under shallow quench/undercooling conditions. Non-isothermal simulations, where meta-stable nematic pre-ordering precedes smectic-A growth, were also conducted and novel non-monotonic phase-transformation kinetics observed.Comment: First revision: 20 pages, 7 figure
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