Clinical outcome of arthroscopic management of isolated posterior cruciate ligament injuries

Background: Isolated posterior cruciate ligament (PCL) injuries are considered as benign and treated conservatively. But studies have shown the long term outcomes of these patients are poor. Aim of this study is to determine the functional outcomes, efficacy, and complication of isolated PCL injuries treated by arthroscopic PCL reconstruction or fixation.Methods: 24 patients, each with an isolated PCL injury, (17 with complete PCL tear and 7 with PCL avulsion fracture) were enrolled in this prospective study. Patients with complete tear underwent PCL reconstruction with hamstring tendon autograft and patients with displaced avulsion fractures underwent arthroscopic fixation with suture bridge technique. Average age at time of surgery was 33 years. Average time from injury to surgery was 1 month. Average follow-up period was 18 months. Patients underwent regular follow-up postoperatively with clinical and radiographic evaluation. Follow-up examinations comprised the Lysholm knee score, the International Knee Documentation Committee (IKDC) score.Results: Mean preoperative Lysholm score for 24 knees was 41; mean postoperative Lysholm score was 90. Eighteen of 24 patients had excellent results, and 4 patients had good results and 2 patients have fare result at final assessment. IKDC ratings showed significant improvements on subsequent follow ups. In final IKDC ratings, 21 patients were assessed as normal or near normal (grade A or B).Conclusions: The short-term follow-up, analytical results showed good function after arthroscopic management in isolated PCL injuries. Hence we recommend surgical intervention in isolated PCL injuries

Clinical results of arthroscopic all-inside meniscal repair

Background: The meniscus plays a key role in normal knee function. Recently, meniscal repair has become an important mode treatment for meniscal tears. Compared to open surgery, arthroscopic meniscal repair has become popular because of shorter duration of the surgery, the smaller incision and better accessibility to the torn portion, which is particularly difficult during open Surgery. Inside-out, outside-in, and all-inside arthroscopic techniques are widely used. Arthroscopy by all inside meniscal repair has the lowest neurovascular injury rate. In this study we have evaluated clinical outcomes of arthroscopic all inside menisceal repair technique.Methods: This study prospectively evaluated 24 consecutively treated patients to determine the effectiveness of arthroscopic meniscal repair using the Fast Fix repair system. Average age of patients at the time of surgery was 24.The inclusion criteria for this study were: vertical full thickness tear >10 mm in length; location of the meniscal tear < 6 mm from the menisco-capsular junction, no former meniscus surgery; and no evidence of arthritis during arthroscopy, absence of complex menisceal tear. Anterior cruciate ligament (ACL) deficient knees were reconstructed using a hamstring autograft or BTB graft at the time of the meniscal repair. Follow-up examinations consisted of IKDC score, Lysholm knee score, Tegner activity score.Results: After an average of 2 years follow-up, no symptoms of meniscal tears were found in 22/24 of the cases. For patients with isolated meniscal repair or concurrent ACL reconstruction, IKDC score, Lysholm and Tegner activity scores had significantly improved postoperatively. One patient had retear for which partial medial meniscectomy was done No neurovascular or other major complications were directly associated with the use of this system.Conclusions: Arthroscopic all-inside repair using the all inside device appears to be a safe and effective surgery to preserve meniscus

Effect of impact shock on extremophilic Halomonas gomseoemensis EP-3 isolated from hypersaline sulphated lake Laguna de Peña Hueca, Spain

The geologic histories of planetary surfaces reveal that Earth and Mars have been pummeled by cataclysmic impact events. The surface of Mars has been perused to have an impact origin for its hemispheric dichotomy. The spallation during impact events causes the interplanetary transfer of material from Mars to Earth or Mars to Phobos/Deimos. Assessing the survival of micro-organisms in impact conditions is critical for the development of planetary protection strategies for future missions. Shock waves are generated during such major impact events. The objective of the present investigation was to explore the microbial diversity of the hypersaline sulphated Laguna de Peña Hueca, Spain and to study the effect of shock waves on extremophilic bacteria isolated from the lake. Peña Hueca is a hypersaline sulphated lagoon rich in Mg–Na–SO4–Cl, epsomite and hexahydrate and it potentially serves as Planetary field analogue site for Martian chloride deposits and salt-rich subsurface brines of Ocean worlds like Enceladus and Europa. The microbial community structure of the lagoon was studied by 16S rRNA metagenomic sequencing. The phylogenetic studies indicated the presence of phyla Euryarchaeota, Proteobacteria, and Bacteroides in the hypersaline brines of the lagoon. The anoxic sediments of Peña Hueca showed the presence of Haloanaerobiaeta and Hadesarchaeota including the anoxic genus of Haloanaerobium, Desulfosalsimonas and Desulfovermiculum. The effect of impact shock on the halophilic bacterium Halomonas gomseomensis EP-3 isolated from Laguna de Peña Hueca was studied in a Reddy shock tube. The halophilic bacterium was exposed to shock waves at a peak shock pressure of 300 ​kPa and a temperature of 400 ​K. The results of shock recovery experiments of halophilic bacteria reveal 97% killing at 300 ​kPa and Mach number of 1.47 in comparison with Bacillus sp. This study indicates that gram-positive spore-forming Bacillus sp. are better adapted to survival in impact shock waves in comparison to non-sporulating halophiles. In the current study, we present the first report on response of halophiles in impact shock. Furthermore, we demonstrate a novel application of the simple handheld Reddy shock tube in astrobiology. The survival studies of halophiles isolated from terrestrial analogue sites in impact shock can provide valuable insights in astrobiology and microbial physiology in impact shock stress

Emodin Suppresses Migration and Invasion through the Modulation of CXCR4 Expression in an Orthotopic Model of Human Hepatocellular Carcinoma

10.1371/journal.pone.0057015PLoS ONE83

Clinical results of arthroscopic all-inside meniscal repair

Background: The meniscus plays a key role in normal knee function. Recently, meniscal repair has become an important mode treatment for meniscal tears. Compared to open surgery, arthroscopic meniscal repair has become popular because of shorter duration of the surgery, the smaller incision and better accessibility to the torn portion, which is particularly difficult during open Surgery. Inside-out, outside-in, and all-inside arthroscopic techniques are widely used. Arthroscopy by all inside meniscal repair has the lowest neurovascular injury rate. In this study we have evaluated clinical outcomes of arthroscopic all inside menisceal repair technique.Methods: This study prospectively evaluated 24 consecutively treated patients to determine the effectiveness of arthroscopic meniscal repair using the Fast Fix repair system. Average age of patients at the time of surgery was 24.The inclusion criteria for this study were: vertical full thickness tear >10 mm in length; location of the meniscal tear < 6 mm from the menisco-capsular junction, no former meniscus surgery; and no evidence of arthritis during arthroscopy, absence of complex menisceal tear. Anterior cruciate ligament (ACL) deficient knees were reconstructed using a hamstring autograft or BTB graft at the time of the meniscal repair. Follow-up examinations consisted of IKDC score, Lysholm knee score, Tegner activity score.Results: After an average of 2 years follow-up, no symptoms of meniscal tears were found in 22/24 of the cases. For patients with isolated meniscal repair or concurrent ACL reconstruction, IKDC score, Lysholm and Tegner activity scores had significantly improved postoperatively. One patient had retear for which partial medial meniscectomy was done No neurovascular or other major complications were directly associated with the use of this system.Conclusions: Arthroscopic all-inside repair using the all inside device appears to be a safe and effective surgery to preserve meniscus

Assessment of dual inhibitory activity of epifriedelanol isolated from Cayratia trifolia against ovarian cancer

Cayratia trifolia is used as diuretic, in tumors, neuralgia and splenopathy. However, compounds depicting anti-ovarian cancer activities from this plant source have not yet been identified and structurally characterized till date. In the present study, X-ray structure of epifriedelanol, a bioactive compound, isolated from the ethanolic extract of the C. trifolia and its binding affinities against a few proteins (HER2, EGFR and CXCR4) that are reported to get overexpressed under ovarian cancer had been thoroughly studied by using molecular docking means. Binding affinities of the compound vis-à-vis that of carboplatin, a FDA approved drug to the ovarian cancer, to interact with the protein targets are quite impressive. The drug-likeness properties of the epifriedelanol and scope to develop the compound as a potent anti-ovarian cancer drug are discussed in this paper

Advancements in the Synthesis and Functionalization of Zinc Oxide-Based Nanomaterials for Enhanced Oral Cancer Therapy

The fabrication of zinc oxide-based nanomaterials (including natural and synthetic polymers like sulfated polysaccharide, chitosan, and polymethyl methacrylate) has potential to improve oral cancer treatment strategies. This comprehensive review explores the diverse synthesis methods employed to fabricate zinc oxide nanomaterials tailored for oral cancer applications. Several synthesis processes, particularly sol–gel, hydrothermal, and chemical vapor deposition approaches, are thoroughly studied, highlighting their advantages and limitations. The review also examines how synthesis parameters, such as precursor selection, the reaction temperature, and growth conditions, influence both the physicochemical attributes and biological efficacy of the resulting nanomaterials. Furthermore, recent advancements in surface functionalization and modification strategies targeted at improving the targeting specificity and pharmaceutical effectiveness of zinc oxide-based nanomaterials in oral cancer therapy are elucidated. Additionally, the review provides insights into the existing issues and prospective views in the field, emphasizing the need for further research to optimize synthesis methodologies and elucidate the mechanisms underlying the efficacy of zinc oxide-based nanoparticles in oral cancer therapy

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Not AvailableCompared to the clinical sector, the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in the food sector is relatively low. However, their presence in seafood is a significant public health concern. In India, fish and fishery products are maximally manually handled compared to other food products. In this study, 498 fish samples were collected under various conditions (fresh, chilled or dressed) and representatives from their surroundings. These samples were screened for the prevalence of Staphylococcus aureus, determining its antimicrobial resistance, MRSA and genetic profile. It is observed that 15.0% and 3.0% of the total samples were screened positive for S. aureus and MRSA, respectively. The S. aureus strain MRSARF-10 showed higher resistance to linezolid, co-trimoxazole, cefoxitin, ofloxacin, gentamicin, rifampicin, ampicillin/sulbactam and Piperacillin–tazobactam. This MRSA, spa type t021 and SCCmec type V strain isolated from dried ribbon fish (Family Trachipteridae) carried virulence factors for exoenzymes such as aureolysin, serine, toxin genes and a novel MLST ST 243, as revealed from its draft-genome sequence. This highly pathogenic, multidrug-resistant and virulent S. aureus novel strain is circulating in the environment with chances of spreading among the seafood workers and the environment. It is further suggested that Good Hygienic Practices recommended by World Health Organization need to be followed during the different stages of seafood processing to provide pathogen-free fish and fishery products to the consumers.Not Availabl